Identification of compounds from non-polar fractions of Blechnum spp and a multitarget approach involving enzymatic modulation and oxidative stress

Abstract

These findings encourage the search for multibinding natural products, mainly from B. brasiliense DCM fraction.

Phytochemical Characterization and In Vitro and In Silico Biological Studies from Ferns of Genus Blechnum (Blechnaceae, Polypodiales)

“…In addition, the results of B. penna-marina are moderately comparable with the species Aristotelia chilensis “maqui”, which represents a raw material endemic to Chile and is known as a standard among the rich sources of antioxidants and registers an ORAC value of 3900 to 29,600 µmol Trolox/g [ 47 ]. The antioxidant properties of the four study species are potent and comparable to the significant effects reported for B. binervatum, B. brasiliense , B. occidentale and B. orientale [ 33 , 35 , 48 ].…”

“…Most of the secondary metabolites reported in B. chilense, B. hastatum, B. magellanicum and B. penna-marina species are grouped in phenolic compounds and organic acids that have also been reported in species such as B. orientale, B. novae-zelandiae, B. occidentale and B. binervatum [ 32 , 33 , 34 , 35 , 36 ]. In the four study species, the compounds neophytadiene, phytol (3,7,11,15-tetramethyl-2-hexadecen-1-ol), isophytol (3,7,11,15-tetramethyl-1-hexadecen-3-ol), ecdysone, 2-deoxyecdysone (2-deoxycrusteecdysone), ponasterone, shidasterone and β-sitosterol (stigmast-5-en-3-ol) have also been reported [ 35 , 37 , 38 , 39 , 40 ]. Flavonoid-like compounds present in species of the genus Blechnum are also shared by species of the genera Pteris and Pteridium of the family Pteridaceae [ 41 ].…”

Terpenic Compounds in Chilean Species of the Genus Blechnum (Pteridophyta: Blechnaceae) with Neuroprotective Potential

“…In fern species of the genus Blechnum phytoecdysteroids have been isolated as neophytadiene (1), isophytol (2), phytol (3), ecdysone (4), ponasterone (5), shidasterone (6), β-sitosterol ( 7), and 2deoxycrusteecdysone (8) [3,4], however, compounds 1, 2 and 3 (Figure 1) report higher abundance and biological activity [5][6][7] in comparison with other compounds (Figure 2). Gas chromatography techniques coupled to a mass spectrometry detector (GC-MS) or a tandem ionization electrospray network detector UHPLC-DAD-ESI-MS/MS, constitute the robust techniques for the identification and quantification of secondary metabolites present in complex extracts from ferns and related plants, made from different polar and non-polar organic solvents [3,8].…”

“…As a result of these findings, it has been possible to identify several terpenic compounds in species of the genus Blechnum, being able to name among those that were expressed in greater measure the neofitadiene, isofitol, and phytol. The applications for medicine that extracts containing these types of terpenic compounds may have are wide, given the range of biological activity that these phytochemicals present [3].…”

Phloroglucinol derivatives from Hypericum species induce in vitro proliferation of cells involved in the wound healing process

“…After 24 h of incubation, the treatment was withdrawn, 100 µL of MTT solution (0.25 mg/mL) were added to each well and plates were incubated for 3 h at 37 °C. Formazan crystals formed by tetrazolium cleavage were dissolved with DMSO (100 µL) and the absorbance was measured using a microplate reader at 570 with background subtraction at 630 nm (Andrade et al, 2016, Andrade et al, 2017, Siqueira et al, 2017. Cell viability was calculated as a percentage in relation to control.…”