Phloroglucinol derivatives from Hypericum species induce in vitro proliferation of cells involved in the wound healing process

Abstract

The genus Hypericum (Hypericaceae) is a recognized source of therapeutic agents, being some species widely used due to their wound healing properties. In a previous study, south Brazilian species H. caprifoliatum, H. carinatum, H. connatum, H. myrianthum and H. polyanthemum demonstrated potential to induce proliferation of keratinocytes. In the present study, the effect of phloroglucinol derivatives isolated from Hypericum on cell proliferation of human keratinocytes, fibroblasts and stem cells was investigated. The better results, determined by the MTT assay, were achieved with cariphenone B at concentrations of 0.01 and 0.1 µM (122.3% and 114%, respectively) in HaCaT cells. Uliginosin B was able to induce the proliferation of mesenchymal stem cells (129% at 10 µM) and MRC5 fibroblasts (152.5% at 5 µM). These findings confirm the capacity of phloroglucinol derivatives to induce the in vitro cellular proliferation and reinforce the importance of Hypericum species as potential sources of wound healing compounds.

Hypericum genus cosmeceutical application – A decade comprehensive review on its multifunctional biological properties

“…The results mentioned above have shown the interesting skin cancer treatment properties of these biomolecules, with no significant cytotoxic effect (Bridi et al, 2019(Bridi et al, , 2017.…”

Comparison of the effect of Triticum Vulgare and Hyperici Oleum on healing in traumatic membrane perforation: an animal study

“…TV and HO are frequently used in traditional medicine due to their tissue repair-accelerating effect [18,19]. TV and HO induce the active cells in wound healing process, especially fibroblast and endothelium, and thus, affect wound healing [18,20]. Thus, the fact that TM perforations can also have positive effects on the healing process should not be neglected.…”

<em>Hypericum perforatum</em> and <em>Azadirachta indica</em> (Neem) oil in the management of chronic leg ulcers: An uncontrolled retrospective observational case review

“…The extracts of Hypericum perforatum and Azadirachta indica show effects in different kinds of dermatological conditions. [10][11][12] Increasing knowledge about pharmacological activities of its characteristic ingredients, such as hypericin and hyperforin, has given new impetus to investigate the potential of topical preparations in dermatological problems of current interest such as skin diseases like atopic dermatitis, psoriasis, herpes infections, nonmelanoma skin cancer and last but not least in skin care. 10 Different types of skin wounds like surgical wounds, 3,9 burns, 4,13 diabetic foot ulcers, 7,8,14 vascular/venous ulcers, pressure sores 15 and calcinosis-related skin ulcers 16 also seem to respond to the pharmacological active ingredients of Hypericum perforatum and Ayadirachta indica oil.…”

“…16 Bridi et al re-cently showed the in vitro capacity of phloroglucinol derivatives from Hypericum species to induce proliferation of cells involved in the wound healing process, therefore reinforcing the importance of Hypericum species as potential source of wound healing compounds. 12 Being a simple wound dressing, no specific training or equipment is required. Hypericum perforatum and Neem oil therefore might be of use as an additional, simple and cost-effective local treatment option in non-healing wounds.…”