Identification of common allergenic structures in mugwort and ragweed pollen☆☆☆★★★

Hirschwehr, Reinhold; Heppner, Claudia; Spitzauer, Susanne; Sperr, Wolfgang R.; Valent, Peter; Berger, Uwe; Horak, Friedrich; Jäger, Siegfried; Kraft, Dietrich; Valenta, Rudolf

doi:10.1016/s0091-6749(98)70384-7

Cited by 89 publications

(52 citation statements)

References 44 publications

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“…Although the molecular weight of Art v 1 is 13-16 kDa, it is migrating between 24 -28 kDa in SDS-PAGE, which results from this rigid and glycosylated C-terminal domain (11). SDS-PAGE and IgE immunoblot experiments using ragweed pollen extract revealed the presence of a glycoprotein similar in size and migrating with the same diffuse double band pattern as Art v 1 (1,2). Nevertheless, no homologue to Art v 1 in ragweed pollen has been described until now.…”

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confidence: 99%

“…These include Ambrosia (ragweed), Artemisia (mugwort), Helianthus (sunflower), and Parthenium (feverfew). It was further demonstrated that sera of mugwort allergic patients show considerable cross-reactivity with ragweed pollen extracts (1,2). IgE-binding to mugwort allergens in immunoblots was inhibited effectively by ragweed pollen extract (1), which indicates close homology of the essential allergens in ragweed and mugwort pollen.…”

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A New Allergen from Ragweed (Ambrosia artemisiifolia) with Homology to Art v 1 from Mugwort

Léonard

Wopfner

Pabst

et al. 2010

Journal of Biological Chemistry

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Art v 1, the major pollen allergen of the composite plant mugwort (Artemisia vulgaris) has been identified recently as a thionin-like protein with a bulky arabinogalactan-protein moiety. A close relative of mugwort, ragweed (Ambrosia artemisiifolia) is an important allergen source in North America, and, since 1990, ragweed has become a growing health concern in Europe as well. Weed pollen-sensitized patients demonstrated IgE reactivity to a ragweed pollen protein of apparently 29 -31 kDa. This reaction could be inhibited by the mugwort allergen Art v 1. The purified ragweed pollen protein consisted of a 57-amino acidlong defensin-like domain with high homology to Art v 1 and a C-terminal proline-rich domain. This part contained hydroxyproline-linked arabinogalactan chains with one galactose and 5 to 20 and more ␣-arabinofuranosyl residues with some ␤-arabinoses in terminal positions as revealed by high field NMR. The ragweed protein contained only small amounts of the single hydroxyproline-linked ␤-arabinosyl residues, which form an important IgE binding determinant in Art v 1. cDNA clones for this protein were obtained from ragweed flowers. Immunological characterization revealed that the recombinant ragweed protein reacted with >30% of the weed pollen allergic patients. Therefore, this protein from ragweed pollen constitutes a novel important ragweed allergen and has been designated Amb a 4.The pollen of common ragweed (Ambrosia artemisiifolia) is a major cause of hay fever and associated asthma in Northern America. During the past few decades, ragweed has started to spread in many parts of central Europe, where it has become a serious health problem in the sensitized population. Several initiatives have formed to prevent further spread in e.g. France, Austria or southern Germany. The Compositae (or Asteraceae) family is one of the largest families of flowering plants, but only a few are important allergenic sources. These include Ambrosia (ragweed), Artemisia (mugwort), Helianthus (sunflower), and Parthenium (feverfew). It was further demonstrated that sera of mugwort allergic patients show considerable cross-reactivity with ragweed pollen extracts (1, 2). IgE-binding to mugwort allergens in immunoblots was inhibited effectively by ragweed pollen extract (1), which indicates close homology of the essential allergens in ragweed and mugwort pollen. So far, six groups of allergens have been identified in ragweed pollen. Most patients were classified as ragweed allergic if they reacted with the pectate lyases of the Amb a 1/2 group (3, 4). The homologous pectate lyase Art v 6 in mugwort has been reported to play only a minor role in allergic disease. Amb a 6 (lipid transfer protein), Amb a 8 (profilin), Amb a 9 and Amb a 10 (both calcium-binding proteins) are small proteins belonging to the group of well known cross-reactive pan-allergens (1, 4 -8). Amb a 7 and the fragment Amb a 3 are plastocyanins and are described only as minor ragweed allergens (9).In mugwort pollen, the major allergen is Art v 1, a protein w...

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A New Allergen from Ragweed (Ambrosia artemisiifolia) with Homology to Art v 1 from Mugwort

Léonard

Wopfner

Pabst

et al. 2010

Journal of Biological Chemistry

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“…Ragweed and mugwort are botanically closely related as both belong to the subfamily Asteroideae of the Asteraceae plant family. In areas with coexposure to both pollen species, concomitant sensitization and clinical reactivity to either weed pollen has been observed (10). The pollen seasons of mugwort and ragweed overlap, and it is nearly impossible to diagnose the primary sensitizing allergen source by using ragweed and mugwort pollen extracts for in vitro or in vivo testing.…”

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“…However, for specific immunotherapy, it is of relevance whether these patients are actually sensitized to both weeds (cosensitized) or whether they are sensitized to one weed and cross-react to the other weed (crosssensitized) (11). Panallergens present in ragweed and mugwort pollen only to a minor extent account for the cross-reactivity of IgE Abs (10,(12)(13)(14). Amb a 1 is another conceivable candidate molecule for causing cross-reactivity, because mugwort pollen contains a homologous allergen, Art v 6, with 65% amino acid sequence identity and 85% amino acid similarity (12,13,15).…”

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Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Jahn‐Schmid

Hauser

Wopfner

et al. 2012

The Journal of Immunology

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Ragweed and mugwort are closely related weeds that represent the major cause of pollen allergy in late summer. Concomitant sensitization and clinical cross-reactivity frequently occur in subjects who are coexposed to both pollen species, and have implications for diagnosis and specific immunotherapy. Molecules involved in this cross-reactivity might be Amb a 1, the major ragweed pollen allergen, and Art v 6, a highly homologous allergen from mugwort. Therefore, we investigated the IgE and T cell response to Art v 6 of 60 weed pollen-allergic patients and assessed its immunological cross-reactivity with Amb a 1. Results of ELISA inhibition experiments suggested that both allergens are largely cross-reactive, but Amb a 1 possesses more IgE epitopes than Art v 6. In patients with IgE to both allergens, Amb a 1-induced T cell lines and clones responded weakly to Art v 6. Moreover, Art v 6-induced T cell lines responded stronger to Amb a 1. T cell epitope mapping of Art v 6 revealed that it contains only a few cross-reactive epitopes, which is opposed to the multiple T cell-activating regions present in Amb a 1. In summary, Amb a 1 can elicit more diverse allergen-specific IgE and T cell responses than Art v 6 and dominates the cross-reactivity with its homolog. Nevertheless, Art v 6 can act as a primary sensitizing allergen in areas with high mugwort pollen exposure, and consequently may facilitate sensitization to Amb a 1 by epitope cross-recognition of T and B cells.

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“…), and nettle (Urtica sp.). This is a mixed group with several crossreacting allergens (Asero et al 2000;Hirschwehr et al 1998). Apple (Mal d 1) (Fritsch et al 1998) Celery (Api g 1) (Bohle et al 2003) Hazelnut (Cor a 1) (Lüttkopf et al 2002) Cherry (Pru av 1) (Scheurer et al 1999 Pear (Pyr c 5) (Karamloo et al 2001) Each group has a different flowering period.…”

Section: Introductionmentioning

confidence: 99%

Strategies for prevention and mitigation of hay fever

Schenk¹,

Vliet²,

Smulders³

et al. 2006

Allergy Matters

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Seasonal allergic rhinitis is a widespread disorder in westernized countries. It has a strong negative impact on the patient's health-related quality of life and is accompanied by considerable medical and economic costs. Currently, the socioeconomic interest in prevention is growing. Reducing or avoiding allergen exposure may result in a significant reduction of sensitization and mitigation of allergic complaints, and this reduction is in addition to existing medical prevention strategies. It will require good communication to (potential) patients on the importance and possibilities for reducing the contact with allergenic pollen. Longterm pollen monitoring and forecasts of the flowering period of allergenic plants are important tools in this respect. Reducing the allergenic load may prove an alternative prevention strategy. Pollen sources are heterogeneously distributed throughout the landscape and may be avoided or selectively removed. The use of selection, conventional breeding strategies or genetic modification may reduce the allergenicity of the pollen sources. Strategies that involve genetic modification are, however, likely to raise some public concern and acceptability of these strategies has to be considered.

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Identification of common allergenic structures in mugwort and ragweed pollen☆☆☆★★★

Cited by 89 publications

References 44 publications

A New Allergen from Ragweed (Ambrosia artemisiifolia) with Homology to Art v 1 from Mugwort

A New Allergen from Ragweed (Ambrosia artemisiifolia) with Homology to Art v 1 from Mugwort

Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Strategies for prevention and mitigation of hay fever

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