Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Jahn‐Schmid, Beatrice; Hauser, Michael; Wopfner, Nicole; Briza, Peter; Berger, Uwe; Asero, Riccardo; Ebner, Christof; Ferreira, Fátima; Bohle, Barbara

doi:10.4049/jimmunol.1102445

Cited by 49 publications

(45 citation statements)

References 28 publications

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“…Amb a 1 shares 65% amino acid sequence identity with the homologous allergen Art v 6 from mugwort pollen [10], also belonging to the PL allergen family. This results in cross-reactivity at the B and T cell level [11]. Amb a 1 appears to be the primary sensitizer for most patients; nevertheless, Art v 6 also has the potential to sensitize susceptible individuals in areas with high mugwort pollen exposure [11].…”

Section: Introductionmentioning

confidence: 99%

“…This results in cross-reactivity at the B and T cell level [11]. Amb a 1 appears to be the primary sensitizer for most patients; nevertheless, Art v 6 also has the potential to sensitize susceptible individuals in areas with high mugwort pollen exposure [11]. …”

Section: Introductionmentioning

confidence: 99%

“…More than 20 Amb a 1 T cell epitopes have been described, including 3 immunodominant epitopes corresponding to residues 178–189, 199–216, 343–357, which are located in the C-terminal alpha chain [13]. Five cross-reactive regions, including 2 immunodominant Amb a 1 epitopes, have been described for Art v 6 [11]. Nevertheless, it remains unclear which of the two Amb a 1 domains is responsible for the molecule´s allergenic properties.…”

Section: Introductionmentioning

confidence: 99%

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T Cell Epitope-Containing Domains of Ragweed Amb a 1 and Mugwort Art v 6 Modulate Immunologic Responses in Humans and Mice

Sancho¹,

Wallner²,

Hauser³

et al. 2017

PLoS ONE

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BackgroundRagweed (Ambrosia artemisiifolia) and mugwort (Artemisia vulgaris) are the major cause of pollen allergy in late summer. Allergen-specific lymphocytes are crucial for immune modulation during immunotherapy. We sought to generate and pre-clinically characterise highly immunogenic domains of the homologous pectate lyases in ragweed (Amb a 1) and mugwort pollen (Art v 6) for immunotherapy.MethodsDomains of Amb a 1 (Amb a 1α) and Art v 6 (Art v 6α) and a hybrid molecule, consisting of both domains, were designed, expressed in E. coli and purified. Human IgE reactivity and allergenicity were assessed by ELISA and mediator release experiments using ragweed and mugwort allergic patients. Moreover, T cell proliferation was determined. Blocking IgG antibodies and cytokine production in BALB/c mice were studied by ELISA and ELISPOT.ResultsThe IgE binding capacity and in vitro allergenic activity of the Amb a 1 and Art v 6 domains and the hybrid were either greatly reduced or abolished. The recombinant proteins induced T cell proliferative responses comparable to those of the natural allergens, indicative of retained allergen-specific T cell response. Mice immunisation with the hypoallergens induced IL-4, IL-5, IL-13 and IFN-γ production after antigen-specific in vitro re-stimulation of splenocytes. Moreover, murine IgG antibodies that inhibited specific IgE binding of ragweed and mugwort pollen allergic patients were detected.ConclusionAccumulation of T cell epitopes and deletion of IgE reactive areas of Amb a 1 and Art v 6, modulated the immunologic properties of the allergen immuno-domains, leading to promising novel candidates for therapeutic approach.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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T Cell Epitope-Containing Domains of Ragweed Amb a 1 and Mugwort Art v 6 Modulate Immunologic Responses in Humans and Mice

Sancho¹,

Wallner²,

Hauser³

et al. 2017

PLoS ONE

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“…10 Among those, the pectate lyase Amb a 1 is currently considered the most allergenic molecule, with greater than 90% of patients with ragweed allergy sensitized to it. [11][12][13] Ragweed pollen also contains minor allergens, such as Amb a 3 and Amb a 7 (plastocyanins), 14 Amb a 4 (homolog to the major mugwort allergen Art v 1), 15 Amb a 5 (an approximately 5-kDa allergen), 16 Amb a 6 (a nonspecific lipid transfer protein), Amb a 8 (profilin), and Amb a 9 and Amb a 10 (calcium-binding proteins) panallergens. 17 Importantly, however, there is clear evidence that some patients with ragweed allergy exhibit a strong IgE reactivity against various ragweed pollen proteins distinct from known allergens (unpublished results).…”

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confidence: 99%

Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed

Bouley¹,

Groeme²,

Mignon

et al. 2015

Journal of Allergy and Clinical Immunology

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“…A comparison between Amb a 1 and the closely related weed allergen Art v 6 revealed that both allergens are cross-reactive [47], but Amb a 1 possesses more IgE epitopes and is a better T-cell stimulant [48]. Another study investigating the cross-reactivity between Amb a 1 and homolog pectate lyase allergens from the botanically unrelated Cupressaceae family (Cry j 1, Jun a 1, and Cup a 1) revealed no significant cross-reactivity between these 2 families [18].…”

Section: Major Ragweed Pollen Allergensmentioning

confidence: 99%

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

Chen

Marusciac

Tamas

et al. 2018

Int Arch Allergy Immunol

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Ambrosia artemisiifolia, also known as common or short ragweed, is an invasive annual flowering herbaceous plant that has its origin in North America. Nowadays, ragweed can be found in many areas worldwide. Ragweed pollen is known for its high potential to cause type I allergic reactions in late summer and autumn and represents a major health problem in America and several countries in Europe. Climate change and urbanization, as well as long distance transport capacity, enhance the spread of ragweed pollen. Therefore ragweed is becoming domestic in non-invaded areas which in turn will increase the sensitization rate. So far 11 ragweed allergens have been described and, according to IgE reactivity, Amb a 1 and Amb a 11 seem to be major allergens. Sensitization rates of the other allergens vary between 10 and 50%. Most of the allergens have already been recombinantly produced, but most of them have not been characterized regarding their allergenic activity, therefore no conclusion on the clinical relevance of all the allergens can be made, which is important and necessary for an accurate diagnosis. Pharmacotherapy is the most common treatment for ragweed pollen allergy but fails to impact on the course of allergy. Allergen-specific immunotherapy (AIT) is the only causative and disease-modifying treatment of allergy with long-lasting effects, but currently it is based on the administration of ragweed pollen extract or Amb a 1 only. In order to improve ragweed pollen AIT, new strategies are required with higher efficacy and safety.

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Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Cited by 49 publications

References 28 publications

T Cell Epitope-Containing Domains of Ragweed Amb a 1 and Mugwort Art v 6 Modulate Immunologic Responses in Humans and Mice

T Cell Epitope-Containing Domains of Ragweed Amb a 1 and Mugwort Art v 6 Modulate Immunologic Responses in Humans and Mice

Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed

Ragweed Pollen Allergy: Burden, Characteristics, and Management of an Imported Allergen Source in Europe

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