Identification of Cashew (Anacardium Occidentale L.) Seedlings With Rapd Markers

Neto, Silva; Maruta, Isao; Takaiwa, Fumio; Oono, K.; Matsumoto, K.

doi:10.17660/actahortic.1995.370.3

Cited by 8 publications

(4 citation statements)

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“…In the present study, the total number of bands ranged from 4-15 with an average of 7.66 bands per primer and the maximum number of bands being produced by primer OU-34. Neto Silva et al (1995) observed a total of 27 amplification products with 0-4 bands per primer using six RAPD primers. In the present investigation, RAPD produced as a whole 94 polymorphic amplicons out of total 107 scorable amplified products resulting 87.85% polymorphism.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, RAPD markers were potent enough to discriminate all test genotypes beyond 71% phenon level. Neto Silva et al (1995) used RAPD markers in cashew to distinguish four dwarf cashew seedlings in Brazil. Similarly, Thimmappaiah et al (2009) reported clear distinction of 67 accessions out of which NRC-142 and NRC-12 emerged as highly divergent while, NRC-231 and NRC-232 were genetically similar among 100 accessions of cashew.…”

Section: Resultsmentioning

confidence: 99%

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Molecular Assessment of Genetic Diversity and Identification of Elite Cashew Hybrids

Sethi

Tripathy

Lenka

et al. 2016

ijh

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Genetic diversity among 20 cashew hybrids and their eight parents were assessed using 15 RAPD primers which resulted 1742 alleles with an average of 62.21alleles per genotype. RAPD profiles revealed 94 polymorphic bands out of total 107 scorable bands resulting 87.85% polymorphism. On an average 7.66 bands were produced with a range of 4-15 amplicons per primer. The maximum number (14) of polymorphic bands (210 to 3250bp) was produced by primer OU-34 with high polymorphic information content (PIC) and resolving power (Rp) values. Cashew hybrids H 6 and D 19 maintained very high level of average genetic dissimilarity with rest of the test genotypes. The above hybrids comprising the most divergent genetic group (Cluster-I) had shown significantly above average productivity and these could enrich the cashew gene pool for further genetic improvement for nut quality and yield per se. Besides, the genotype-specific bands identified in the present study are useful for identification of cashew genotypes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Molecular Assessment of Genetic Diversity and Identification of Elite Cashew Hybrids

Sethi

Tripathy

Lenka

et al. 2016

ijh

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“…RAPD markers provide a quick and efficient screening of DNA as they require small amount of DNA, involve non-radioactive assay, need a simple experimental set-up, do not require species-specific probe libraries and blotting or hybridization step. 9 RAPD technique has been employed to asses genetic diversity within and among natural populations of a large number of fruit tree species like Mango 10,11 Pistachio 12 Cashew [13][14][15][16][17] and Apricot. 18 ISSR markers are PCR based like RAPD but are more reproducible than RAPD due to their better stringency (high annealing temperature), require no gene sequence information and targets microsatellite motif.…”

Section: International Journal Of Phytocosmetics and Natural Ingrediementioning

confidence: 99%

Molecular characterization of diversity in Annona squamosa L.: A high value tropical medicinal plant

Nagori¹,

Purohit²

2018

Int J Phytocos Nat Ingred

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Introduction: Annona squamosa L. commonly known as custard apple, is an important under-utilized fruit species from tropical and subtropical parts of India with high potential as commercial horticultural crop. The aim of this study was to determine the genetic diversity among populations spread across districts of Udaipur, Rajsamand and Chittaurgarh falling within Aravallis in Rajasthan. In all, 21 populations were identified and subjected to molecular evaluation using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. The cumulative analysis carried out on the basis of RAPD and ISSR data sets revealed 73.91% polymorphism. Methods: A total of 251 amplicons were produced using 19 RAPD and 15 ISSR primers. The pairwise distance matrix calculated by Dice's coefficient showed a distance range of 0.67 to 0.95 when computed using cumulative data set. Results: Characterization of genetic diversity within populations based on combined data set showed that percentage of polymorphism bands (PPB) ranged from 29.19 to 50.93. Nei's gene diversity (H E) values varied from 0.1002 to 0.1964 within a total genetic diversity (HT) of 0.2509. The average observed number of alleles per locus (Na) was 1.3602 and Ne was 1.2400. The Shanon's index was 0.2002 (Hpop) and 0.3800 (Hsp) at population and species level, respectively. Conclusion: The moderate gene flow value (Nm=0.59) and significant coefficient of genetic differentiation (G ST =0.458) revealed low level of genetic diversity within population and highly significant genetic differentiation among populations of A. squamosa. The UPGMA dendrogram resolved all the 21 populations representing 3 districts into various clusters according to their genetic distances. The principal component analysis (PCA) supported the UPGMA results and justified the relationship among populations.

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“…Transformation in cashew using Agrobacterium tumefasciens strain EHA-105 has been reported by Kiran et al (2007). Neto et al (1995) showed utility of RAPD markers in distinguishing dwarf seedlings in cashew. Bulk Segregant Analysis (BSA) in germplasm bulks at DCR, Puttur, could identify four RAPD markers linked to economic characters like nut weight and plant stature.…”

Section: Biotechnology In Cashewmentioning

confidence: 99%

Cashew research in India

Bhat,

Nagaraja,

Rupa

2010

J. Hortic. Sci.

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Cashew, after its introduction from Brazil during the 16th Century, has established very well in India. A total of 40 high-yielding varieties have been released so far by the Directorate of Cashew Research, Puttur, and various Agricultural Universities, for cultivation. Of these, 13 are hybrids and 27 are selections. Research achievements in the area of crop improvement, management, protection and post-harvest technology over the last six decades are reviewed and documented here. As India has been importing raw nuts to the tune of 6.5 lakh tons annually to cater the demand of established processing factories, research priorities have been identified to meet the challenges of enhancing production and productivity of cashew in the country.

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Identification of Cashew (Anacardium Occidentale L.) Seedlings With Rapd Markers

Cited by 8 publications

References 0 publications

Molecular Assessment of Genetic Diversity and Identification of Elite Cashew Hybrids

Molecular Assessment of Genetic Diversity and Identification of Elite Cashew Hybrids

Molecular characterization of diversity in Annona squamosa L.: A high value tropical medicinal plant

Cashew research in India

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