Identification of Candidate Odorant Receptors in Asian Corn Borer Ostrinia furnacalis

Abstract

In lepidopteran insects, odorant receptors are involved in the perception of sex pheromones and general odorants. In the Asian corn borer, Ostrinia furnacalis, although several pheromone receptors have been identified, no general odorant receptor has been reported. In this study, an RNA sequencing analysis was carried out to identify the whole repertoire of the odorant receptors expressed in the antennae of O. furnacalis. Among 12 million reads obtained from the antennae of male and female moths, 52 candidate odorant receptors were identified, including 45 novel ones. Expression levels of candidate odorant receptors were estimated by read mapping and quantitative reverse transcription PCR. These analyses confirmed that the expression of the previously identified pheromone receptors was highly male biased. In contrast, none of the newly identified odorant receptors showed male-biased expression. Three of the newly identified odorant receptors showed female-biased expression. Two of them were the most highly expressed odorant receptors in the female antennae, suggesting that they may be involved in the detection of odorants important for the induction of female-specific behaviors such as oviposition site selection. In addition, candidate genes of 21 ionotropic receptors, 5 gustatory receptors, 2 sensory neuron membrane proteins, and 26 odorant degrading enzymes were identified. Our results provide a basis for further analysis of the chemosensory system in the Ostrinia species.

Age influences the olfactory profiles of the migratory oriental armyworm mythimna separate at the molecular level

“…The number of ORs, IRs, OBPs, and CSPs in M. separate is smaller than that in B. mori [19, 45], which was conducted using the whole genome, but was the same as that of other moths that were studied using the same protocol (antennal transcriptome), which included Agrotis ipsilon [46], C. suppressalis [37], H. armigera [47], O. furnacalis [35, 36]. However, the number of GRs and SNMPs was larger than those of most moths studied.…”

“…Amino acid sequence alignment was performed using CLUSTALX [30]. For the phylogenetic analysis, the amino acid sequences of Drosophila melanogaster [31, 32], H. armigera [33], M. sexta [34], Ostrinia furnacalis [35, 36], Chilo suppressalis [37], B. mori [19, 38], S. litura [23], and Heliothis virescens [39] were used. Phylogenetic analyses were conducted using the maximum likelihood method of MEGA 6.0, which was based on the Jones-Taylor-Thornton substitution model, partial deletion gaps with 95% site coverage cutoff, and nearest neighbor interchanges heuristic search [40].…”

Transcriptome sequencing reveals abundant olfactory genes in the antennae of the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

“…; Tanaka et al ), C. suppressalis (Cao et al . ), H. armigera (Zhang et al ) and O. furnacalis (Yang et al ). This suggests there may be other olfactory genes that have not yet been identified in C. medinalis .…”

A Conserved Odorant Receptor Detects the Same 1-Indanone Analogs in a Tortricid and a Noctuid Moth

“…A number of lepidopteran OR gene repertoires have been described, following genome and transcriptome sequencing (Jordan et al, 2009;Grosse-Wilde et al, 2011;Montagné et al, 2012Montagné et al, , 2014Cao et al, 2014;Gu et al, 2014;Liu et al, 2014;Corcoran et al, 2015;Yang et al, 2015;Zhang et al, 2015a,b). In our own transcriptome sequence analyses of the antennae of the codling moth (Cydia pomonella: Tortricidae; Bengtsson et al, 2012) and the cotton leafworm (Spodoptera littoralis: Noctuidae; Legeai et al, 2011;Jacquin-Joly et al, 2012;Poivet et al, 2013) we have identified one OR (OR19) with relatively high sequence similarity in both species.…”