Transcriptome sequencing reveals abundant olfactory genes in the antennae of the rice leaffolder, <i>Cnaphalocrocis medinalis</i> (Lepidoptera: Pyralidae)

Liu, Su; Wang, Wenlong; Zhang, Yuxing; Zhang, Bang‐Xian; Rao, Xiang‐Jun; Liu, Xiaoming; Wang, Dong‐Ming; Li, Shi‐Guang

doi:10.1111/ens.12253

Cited by 20 publications

(23 citation statements)

References 59 publications

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“…There was high homology among the known Orco genes (Jones et al 2005;Xiao et al 2009). In comparison with the number of genes identified in this study, 43 ORs and Orco have been identified in Holotrichia oblita Faldermann , and 46 ORs and Orco have been identified in Cnaphalocrocis medinalis Guenee (Liu et al 2017). We believe that we have identified a sufficient number of genes from the transcriptome.…”

Section: Discussionsupporting

confidence: 48%

Identification and Expression Profiling of Odorant Receptor Protein Genes in Sitophilus zeamais (Coleoptera: Curculionoidea) Using RT-qPCR

et al. 2019

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This study aimed to identify ORs (odorant receptors) and Orco (odorant receptor coreceptor) genes in Sitophilus zeamais Motschulsky (Coleoptera: Curculionoidea), to explore the relative expression levels of these genes in different adult tissues and obtain information on highly expressed receptor proteins. Putative OR and Orco genes were identified from transcriptomic data previously obtained for S. zeamais using bioinformatics methods. Quantitative real-time PCR was used to compare the differences in expression in seven adult tissues (male antennae, female antennae, heads, thoraxes, abdomens, wings, and legs). The candidate OR and Orco gene sequences were analyzed, and the protein physicochemical properties were predicted. We identified 64 OR genes including the Orco gene. Forty-seven OR genes, including Orco, were over expressed in male or female antennae. Seventeen OR genes appeared to be expressed at elevated levels in male antennae. Twenty-nine genes were expressed at significantly elevated levels in female antennae. In total, 11 OR genes were selected for further sequence analysis. The selected proteins were structurally characterized, and bioinformatics analysis was performed. Overall, in this study, candidate ORs of S. zeamais have been identified for the first time, and these ORs could be molecular targets for interference in the insect olfactory system.

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Section: Discussionsupporting

confidence: 48%

Identification and Expression Profiling of Odorant Receptor Protein Genes in Sitophilus zeamais (Coleoptera: Curculionoidea) Using RT-qPCR

et al. 2019

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“…The other HrhoORco and HrhoOR11 with approximately equal expression levels between female and male antennae are likely to function in the detection of food source odors. In addition, HrhoOR37 was orthologous to CmedOR32 according to phylogenetic analysis, and the expression levels of these two OR genes were higher in the FA than in the MA (Liu et al, 2017b). Therefore, we speculate that these two orthologous OR genes may play similar roles in odor detection.…”

Section: Discussionmentioning

confidence: 83%

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Yang¹,

Dong²,

Sun³

et al. 2020

PeerJ

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Insect olfaction and vision play important roles in survival and reproduction. Diurnal butterflies mainly rely on visual cues whereas nocturnal moths rely on olfactory signals to locate external resources. Histia rhodope Cramer (Lepidoptera: Zygaenidae) is an important pest of the landscape tree Bischofia polycarpa in China and other Southeast Asian regions. As a diurnal moth, H. rhodope represents a suitable model for studying the evolutionary shift from olfactory to visual communication. However, only a few chemosensory soluble proteins have been characterized and information on H. rhodope chemoreceptor genes is currently lacking. In this study, we identified 45 odorant receptors (ORs), nine ionotropic receptors (IRs), eight gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) from our previously acquired H. rhodope antennal transcriptomic data. The number of chemoreceptors of H. rhodope was less compared with that found in many nocturnal moths. Some specific chemoreceptors such as OR co-receptor (ORco), ionotropic receptors co-receptor, CO2 receptors, sugar receptors and bitter receptors were predicted by phylogenetic analysis. Notably, two candidate pheromone receptors (PRs) were identified within a novel PR lineage. qRT-PCR results showed that almost all tested genes (22/24) were predominantly expressed in antennae, indicating that they may be important in olfactory function. Among these antennae-enriched genes, six ORs, five IRs and two GRs displayed female-biased expression, while two ORs displayed male-biased expression. Additionally, HrhoIR75q.2 and HrhoGR67 were more highly expressed in heads and legs. This study enriches the olfactory gene inventory of H. rhodope and provides the foundation for further research of the chemoreception mechanism in diurnal moths.

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“…The number of identified PintOBPs was equivalent to that from H . armigera (26) [ 11 ], Dendrolimus kikuchii (27) [ 17 ] and Agrotis ipsilon (33) [ 21 ], and it was significantly greater than that from Cnaphalocrocis medinalis (12) [ 12 ], C . punctiferalis (14) [ 13 ], Manduca sexta (18) [ 43 ] and S .…”

Section: Discussionmentioning

confidence: 99%

Identification of chemosensory genes from the antennal transcriptome of Indian meal moth Plodia interpunctella

et al. 2018

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Olfaction plays an indispensable role in mediating insect behavior, such as locating host plants, mating partners, and avoidance of toxins and predators. Olfactory-related proteins are required for olfactory perception of insects. However, very few olfactory-related genes have been reported in Plodia interpunctella up to now. In the present study, we sequenced the antennae transcriptome of P. interpunctella using the next-generation sequencing technology, and identified 117 candidate olfactory-related genes, including 29 odorant-binding proteins (OBPs), 15 chemosensory proteins (CSPs), three sensory neuron membrane proteins (SNMPs), 47 odorant receptors (ORs), 14 ionotropic receptors (IRs) and nine gustatory receptors (GRs). Further analysis of qRT-PCR revealed that nine OBPs, three CSPs, two SNMPs, nine ORs and two GRs were specifically expressed in the male antennae, whereas eight OBPs, six CSPs, one SNMP, 16 ORs, two GRs and seven IRs significantly expressed in the female antennae. Taken together, our results provided useful information for further functional studies on insect genes related to recognition of pheromone and odorant, which might be meaningful targets for pest management.

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Transcriptome sequencing reveals abundant olfactory genes in the antennae of the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Cited by 20 publications

References 59 publications

Identification and Expression Profiling of Odorant Receptor Protein Genes in Sitophilus zeamais (Coleoptera: Curculionoidea) Using RT-qPCR

Identification and Expression Profiling of Odorant Receptor Protein Genes in Sitophilus zeamais (Coleoptera: Curculionoidea) Using RT-qPCR

Identification and expression profiles of candidate chemosensory receptors in Histia rhodope (Lepidoptera: Zygaenidae)

Identification of chemosensory genes from the antennal transcriptome of Indian meal moth Plodia interpunctella

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