Age influences the olfactory profiles of the migratory oriental armyworm mythimna separate at the molecular level

Feng, Bo; Guo, Qixin; Du, Yanjun

doi:10.1186/s12864-016-3427-2

Cited by 29 publications

(27 citation statements)

References 82 publications

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“…There are three previous studies antennal on transcriptome of M. separata. He et al (2017) identified 126 olfactory genes including 43 ORs, 13 GRs, 16 IR, 37 OBPs, 14 CSPs, and 3 SNMPs [ 58 ], Chang et al (2017) obtained 130 chemosensory genes encoding 71 ORs, 1 GR, 8 IRs, 32 OBPs, 16 CSPs, and 2 SNMPs [ 59 ] and Liu et al (2017) gained 60 ORs, 8 GRs, 24 IRs, 50 OBPs, 22 CSPs and 2 SNMPs [ 60 ]. Compared with Liu’s study which was also on the head transcriptome of M. separate , we identified some novel chemosensory genes and the lengths of the genes were significantly longer.…”

Section: Discussionmentioning

confidence: 99%

Identification of candidate chemosensory genes in Mythimna separata by transcriptomic analysis

Zhao

Liang

et al. 2018

BMC Genomics

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BackgroundThe oriental armyworm, Mythimna separata, is an economically important and common Lepidopteran pest of cereal crops. Chemoreception plays a key role in insect life, such as foraging, oviposition site selection, and mating partners. To better understand the chemosensory mechanisms in M. separata, transcriptomic analysis of antennae, labial palps, and proboscises were conducted using next-generation sequencing technology to identify members of the major chemosensory related genes.ResultsIn this study, 62 putative odorant receptors (OR), 20 ionotropic receptors (IR), 16 gustatory receptors (GR), 38 odorant binding proteins (OBP), 26 chemosensory proteins (CSP), and 2 sensory neuron membrane proteins (SNMP) were identified in M. separata by bioinformatics analysis. Phylogenetic analysis of these candidate proteins was performed. Differentially expressed genes (DEGs) analysis was used to determine the expressions of all candidate chemosensory genes and then the expression profiles of the three families of receptor genes were confirmed by real-time quantitative RT-PCR (qPCR).ConclusionsThe important genes for chemoreception have now been identified in M. separata. This study will provide valuable information for further functional studies of chemoreception mechanisms in this important agricultural pest.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4898-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Identification of candidate chemosensory genes in Mythimna separata by transcriptomic analysis

Zhao

Liang

et al. 2018

BMC Genomics

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“…Phylogenetic analysis of CSPs from different insect species shows that CSPs are highly conserved and possess a characteristic N-terminal signature motif ( Wanner et al, 2004 ; Forêt et al, 2007 ). CSPs perceive and bind external ligands, which result in action potentials that contribute to subsequent behaviors ( Tunstall and Warr, 2012 ; He Y. et al, 2017 ). Previous studies have shown that CSPs are exclusively expressed in the antennae of Sesamia inferens ( Zhang et al, 2014 ), Polistes dominulus ( Calvello et al, 2003 ), Linepithema humile ( Ishida et al, 2002 ), Cerapachys biroi ( Mckenzie et al, 2014 ) and other insect species.…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of the Chemosensory Protein MsepCSP8 From the Oriental Armyworm Mythimna separata

et al. 2018

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Chemosensory proteins (CSPs) play important roles in chemosensation in insects, but their exact physiological functions remain elusive. In order to investigate the functions of CSPs in the oriental armyworm Mythimna separata, in the present study we explored expression patterns and binding characteristics of the CSP, MsepCSP8. The distinctive functions of MsepCSP8 were also validated by RNAi. The results showed that MsepCSP8 shares high sequence similarity with CSPs of other insect family members, including the characteristic four-cysteine signature motif. MsepCSP8 mRNA was specifically expressed in antennae of females at levels well above those in other tissues. Competitive binding assays confirmed that 20 out of 56 ligands bound more strongly to MsepCSP8 at pH 7.4 than at pH 5.0. Protein structure modeling and molecular docking analyses identified amino acid residues involved in binding volatile compounds, and behavioral response experiments showed that M. separata elicited significant responses to five volatiles from compounds displaying high binding affinity to MsepCSP8. MsepCSP8 transcript abundance was decreased by dsMsepCSP8 injection, which affected the behavioral responses of M. separata to representative semiochemicals. Our findings demonstrate that MsepCSP8 likely contributes to mediating responses of M. separata adults to plant volatiles.

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“…Like other moth species, My. separata relies on olfaction for locating food and mates ( He et al, 2017 ). In addition, My.…”

Section: Introductionmentioning

confidence: 99%

Central Projections of Antennal and Labial Palp Sensory Neurons in the Migratory Armyworm Mythimna separata

Zhao

Berg

et al. 2017

Front. Cell. Neurosci.

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The oriental armyworm, Mythimna separata (Walker), is a polyphagous, migratory pest relying on olfactory cues to find mates, locate nectar, and guide long-distance flight behavior. In the present study, a combination of neuroanatomical techniques were utilized on this species, including backfills, confocal microscopy, and three-dimensional reconstructions, to trace the central projections of sensory neurons from the antenna and the labial pit organ, respectively. As previously shown, the axons of the labial sensory neurons project via the ipsilateral labial nerve and terminate in three main areas of the central nervous system: (1) the labial-palp pit organ glomerulus of each antennal lobe, (2) the gnathal ganglion, and (3) the prothoracic ganglion of the ventral nerve cord. Similarly, the antennal sensory axons project to multiple areas of the central nervous system. The ipsilateral antennal nerve targets mainly the antennal lobe, the antennal mechanosensory and motor center, and the prothoracic and mesothoracic ganglia. Specific staining experiments including dye application to each of the three antennal segments indicate that the antennal lobe receives input from flagellar olfactory neurons exclusively, while the antennal mechanosensory and motor center is innervated by mechanosensory neurons from the whole antenna, comprising the flagellum, pedicle, and scape. The terminals in the mechanosensory and motor center are organized in segregated zones relating to the origin of neurons. The flagellar mechanosensory axons target anterior zones, while the pedicular and scapal axons terminate in posterior zones. In the ventral nerve cord, the processes from the antennal sensory neurons terminate in the motor area of the thoracic ganglia, suggesting a close connection with motor neurons. Taken together, the numerous neuropils innervated by axons both from the antenna and labial palp indicate the multiple roles these sensory organs serve in insect behavior.

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Age influences the olfactory profiles of the migratory oriental armyworm mythimna separate at the molecular level

Cited by 29 publications

References 82 publications

Identification of candidate chemosensory genes in Mythimna separata by transcriptomic analysis

Identification of candidate chemosensory genes in Mythimna separata by transcriptomic analysis

Functional Analysis of the Chemosensory Protein MsepCSP8 From the Oriental Armyworm Mythimna separata

Central Projections of Antennal and Labial Palp Sensory Neurons in the Migratory Armyworm Mythimna separata

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