Identification of bioactive ingredients with immuno-enhancement and anti-oxidative effects from Fufang-Ejiao-Syrup by LC–MS n combined with bioassays

Shen, Lijuan; Chen, Houliu; Zhu, Qiu-Fang; Wang, Yong-Yi; Wang, Saisai; Qian, Jing; Wang, Yi; Qu, Haibin

doi:10.1016/j.jpba.2015.09.024

Cited by 27 publications

(18 citation statements)

References 34 publications

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“…1 Collagen is believed to be the main active component in CCA, which is widely used to nourish the blood, optimize the immune response, improve the metabolic balance, delay senescence, and treat gynecologic diseases. [2][3][4][5][6] Because CCA is very effective in practice, in 2015, its yearly production and market sales increased to approximately 6,000 tons and 1.5 billion US dollars, respectively. However, because donkeys are not major livestock animals, the increasing demand for CCA will inevitably cause a shortage of materials.…”

Section: Introductionmentioning

confidence: 99%

Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach

Li¹,

Shi²,

Gong³

et al. 2017

IJN

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Colla corii asini (CCA) is a protein-based traditional Chinese medicine made from donkey skins. Because it has the ability to nourish blood, its demand is increasing rapidly. The shortage of donkey skins increases the risk of the adulteration of CCA products with other animal skins. To ensure the drug efficacy and safety of CCA products, a proteomics technique was applied to reveal proteins in the skins of donkey, horse, cattle, and pig. Species-specific peptides for each animal species were predicted using bioinformatics, and their presence in the skins and gelatin samples was examined by nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS). One unique marker peptide for each animal species was selected to develop an LC-MS/MS multiple reaction monitoring method. The capability of this method to identify donkey, horse, cattle, and pig materials was demonstrated by analyzing in-house-made donkey gelatins containing different amounts of other animal skins and commercial CCA products. The adulteration of non-donkey species could be sensitively detected at a low level of 0.5%. Hybrid animals, such as mules and hinnies, were also differentiated from donkeys. We provide a practical tool for the quality control of CCA products. The strategy can also be used to study other important traditional Chinese medicines which contain animal proteins.

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Section: Introductionmentioning

confidence: 99%

Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach

Li¹,

Shi²,

Gong³

et al. 2017

IJN

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“…Therefore, herbal medicines which can nourish qi and blood, as well as invigorate the spleen and kidney are often used in the clinic to treat blood deficiency and anemia (Hijikata et al, 2009;Wu et al, 2013;Sun et al, 2016). For example, FEJ, the positive control in the study, is a widely used immune-boosting traditional Chinese formula which has been clearly confirmed to promote the recovery of bone marrow hemopoietic activity and enhance the immune function (Liu et al, 2014;Shen et al, 2016). While for SPN, another tonic and hemostatic drug used for more than 400 years, the possible mechanism of the "blood-tonifying" effect is unclear yet.…”

Section: Discussionmentioning

confidence: 94%

Steamed Panax notoginseng Attenuates Anemia in Mice With Blood Deficiency Syndrome via Regulating Hematopoietic Factors and JAK-STAT Pathway

Zhang

Gao

et al. 2020

Front. Pharmacol.

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“…In modern medicine, FEJ is widely used as a supportive reagent for chemotherapy-induced myelosuppression in East Asian countries, including China, Japan, and Korea. We previously reported that FEJ stimulates the hematopoiesis of bone marrow (BM) cells in mice with 5-fluorouracil (5FU)-induced myelosuppression ( Shen et al, 2016 ). Unfortunately, as with other traditional Chinese medicines, there is only a limited amount of information on the beneficial effects and mechanism of FEJ.…”

Section: Introductionmentioning

confidence: 99%

“…FEJ is a multi-component formula that contains Asini Corii Colla, Ginseng Radix et Rhizoma Rubra, Rehmanniae Radix Praeparata, Codonopsis Radix, and Crataegi fructus ( Liu et al, 2014 ). We recently utilized high-performance liquid chromatography–mass spectrometry (HPLC–MS), together with high-resolution mass spectrometry (HR-MS), to further characterize FEJ ( Shen et al, 2016 ). In this previous analysis, 72 phytochemical constituents were identified, including the novel bioactive compounds martynoside, R-notoginsenoside R2 (designated R2), and 20S-ginsenoside Rg2 (designated Rg2).…”

Section: Introductionmentioning

confidence: 99%

“…These three compounds have been shown to have numerous biological effects. Martynoside, identified as a component of Rehmanniae Radix Praeparata ( Shen et al, 2016 ), is a plant phenylpropanoid glycoside ( Figure 1A ). It has several known pharmacological properties, including anti-sports anemia ( Zhu et al, 2010 ) and retardation of skeletal muscle fatigue ( Liao et al, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Profiling Analysis Reveals the Potential Mechanisms of Three Bioactive Ingredients of Fufang E’jiao Jiang During Chemotherapy-Induced Myelosuppression in Mice

Zhang

Hong

et al. 2018

Front. Pharmacol.

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Although multiple bioactive components have been identified in Fufang E’jiao Jiang (FEJ), their hematopoietic effects and molecular mode of action in vivo are still not fully understood. In the current study, we analyzed the effects of martynoside, R-notoginsenoside R2 (R2), and 20S-ginsenoside Rg2 (Rg2) in a 5-fluorouracil-induced myelosuppression mouse model. Bone marrow nucleated cells (BMNCs) counts, hematopoietic progenitor cell colony-forming unit (CFU) assay, as well as flow cytometry analysis of Lin-/c-kit+/Sca-1+ hematopoietic stem cell (HSC) population were conducted, and bone marrow cells were subjected to RNA sequencing. The transcriptome data were processed based on the differentially expressed genes. The results of the analysis show that each of the three compounds stimulates BMNCs and HSC growth, as well as burst-forming unit-erythroid and colony-forming unit granulocyte-monocyte colony expansion. The most relevant transcriptional changes appeared to be involved in regulation of hematopoietic cell lineage, NF-κB and TNF-α signaling, inhibition of inflammation, and acceleration of hematopoietic cell recovery. Notably, the individual compounds shared similar but specified transcriptome profiles. Taken together, the hematopoietic effects for the three tested compounds of FEJ are confirmed in this myelosuppression mouse model. The transcriptome maps of these effects provide valuable information concerning their underlying mechanisms and provide a framework for the continued study of the complex mode of action of FEJ.

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Identification of bioactive ingredients with immuno-enhancement and anti-oxidative effects from Fufang-Ejiao-Syrup by LC–MS n combined with bioassays

Cited by 27 publications

References 34 publications

Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach

Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach

Steamed Panax notoginseng Attenuates Anemia in Mice With Blood Deficiency Syndrome via Regulating Hematopoietic Factors and JAK-STAT Pathway

Transcriptome Profiling Analysis Reveals the Potential Mechanisms of Three Bioactive Ingredients of Fufang E’jiao Jiang During Chemotherapy-Induced Myelosuppression in Mice

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