Transcriptome Profiling Analysis Reveals the Potential Mechanisms of Three Bioactive Ingredients of Fufang E’jiao Jiang During Chemotherapy-Induced Myelosuppression in Mice

Abstract

Although multiple bioactive components have been identified in Fufang E’jiao Jiang (FEJ), their hematopoietic effects and molecular mode of action in vivo are still not fully understood. In the current study, we analyzed the effects of martynoside, R-notoginsenoside R2 (R2), and 20S-ginsenoside Rg2 (Rg2) in a 5-fluorouracil-induced myelosuppression mouse model. Bone marrow nucleated cells (BMNCs) counts, hematopoietic progenitor cell colony-forming unit (CFU) assay, as well as flow cytometry analysis of Lin-/c-kit+/Sca-1+ hematopoietic stem cell (HSC) population were conducted, and bone marrow cells were subjected to RNA sequencing. The transcriptome data were processed based on the differentially expressed genes. The results of the analysis show that each of the three compounds stimulates BMNCs and HSC growth, as well as burst-forming unit-erythroid and colony-forming unit granulocyte-monocyte colony expansion. The most relevant transcriptional changes appeared to be involved in regulation of hematopoietic cell lineage, NF-κB and TNF-α signaling, inhibition of inflammation, and acceleration of hematopoietic cell recovery. Notably, the individual compounds shared similar but specified transcriptome profiles. Taken together, the hematopoietic effects for the three tested compounds of FEJ are confirmed in this myelosuppression mouse model. The transcriptome maps of these effects provide valuable information concerning their underlying mechanisms and provide a framework for the continued study of the complex mode of action of FEJ.

Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway

“…Notoginsenoside R2 (NGR2) is an active saponin molecule extracted from the root of PN. Previous studies showed that it reduced A25-35-induced neuronal apoptosis and inflammation (8), and stimulated the growth of bone marrow nucleated cells and hematopoietic stem cells, as well as burst-forming unit-erythroid and colony-forming unit granulocyte-monocyte colony expansion (9). However, the role of NGR2 in angiogenesis remains unexplored, which is precisely the purpose of this study.…”

Bioinformatics Analysis of miRNAs and mRNAs Network-Xuefu Zhuyu Decoction Exerts Neuroprotection of Traumatic Brain Injury Mice in the Subacute Phase

“…Thereby, discerning miRNAs, related targets genes, and their regulatory signaling pathways are crucial in knowing the common biological development of miRNAs and their actions in the disease process ( Croce, 2009 ; Liu et al, 2014 ). Nowadays, scientists have explored the traditional Chinese Medicine (TCM) pharmacological mechanism and potential therapeutic targets via transcriptomics technologies ( Zhang et al, 2017b ; Xu, 2017 ; Li et al, 2018 ). miRNA-based therapeutics approaches have been assessed at the preclinical and clinical stages.…”

Comparison of blood tonic efficacy and chemical constituents of Kadsura interior A.C. Smith and its closely related species

“…Hence, the body weights, the levels of blood routine indicators like HCT, HGB, and RBC, the thymus and spleen indexes, and the serum levels of hemopoietic growth factors, including IL-3, GM-CSF, and M-CSF, were adopted to evaluate the hematopoietic function [ 37 , 38 ]. FEJ is a well-known and clinically effective proprietary Chinese medicine for the treatment of BD and therefore its clinical dose was used in the positive control of this study [ 39 ]. The observation and body weights of mice were only general indicators, the efficacies of blood tonic should be assessed primarily by reference to the data of blood routines.…”