Identification of Bacteroides species from adult periodontal disease

Duerden, B. I.; Goodwin, Linda; O'Neil, T. C. A.

doi:10.1099/00222615-24-2-133

Cited by 31 publications

(18 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These periodontopathogens are frequently isolated from periodontal pockets of patients with periodontitis, but up to now it has been unclear which species play the main role in pathogenesis. In this study, B. fragilis was used as an example of an opportunistic periodontopathogen, as many reports have documented the isolation of B. fragilis from dental plaque or periodontal pockets of patients with periodontitis (23)(24)(25).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria

2012

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria

2012

View full text Add to dashboard Cite

“…In a series of investigations of anaerobic infections, we have isolated B. ureolyticus from a range of clinical conditions in which it seemed to have a pathogenic role (Duerden et al, 1982(Duerden et al, ,1987Masfari eta]., 1983,1985). In the present study, we examined three groups of isolates-from dental sources, from ulcers and soft-tissue infections (including genital ulcers), and from urethritis-in morphological, biochemical, tolerance and antibiotic susceptibility tests, by GLC analysis of volatile fatty-acid products of metabolism, and by pyrolysis-mass spectrometry (Py-MS), and compared these with reference strains of B. ureolyticus and B. gracilis.…”

Section: Introductionmentioning

confidence: 99%

“…These include : buccal abscesses (Eiken, 1958) ; periodontal disease (Khairat, 1967 ;Duerden et al, 1987) and other oral infections (Schroter and Stawru, 1970;Duerden et al, 1982); soft tissue infection and ulcers, especially peripheral (e.g., varicose) ulcers and gangrene, and ulcerative conditions of the perineum and external genitalia (Labbt: et al, 1977;Duerden et al, 1982;Masfari et al, 1983Masfari et al, , 1985. Fontaine et al (1982,1984) used refined anaerobic transport and culture methods to isolate strains, subsequently confirmed as B. ureolyticus (Fontaine et al, 1986;Taylor et al, 1986) from urethral samples from men with non-gonococcal, non-chlamydia1 urethritis ; successful treatment with metronidazole further supported a pathogenic role for B. ureolyticus in these patients (Hawkins et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

A Comparison of Bacteroides ureolyticus isolates from different clinical sources

Duerden

Eley

Goodwin

et al. 1989

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Summary. Clinical isolates of corroding, gram-negative, anaerobic bacilli (provisionally identified as Bacteroides ureolyticus) from superficial ulcers and soft tissue infections (1 9, non-gonococcal, non-chlamydia1 urethritis (1 2) and adult periodontal disease (14) were compared with reference strains of B. ureolyticus, B. gracilis and Wolinella recta in a series of conventional tests of morphology, biochemical activity, tolerance of dyes and bile salts, and antibiotic sensitivity, gas-liquid chromatographic analysis of metabolic products, and in whole-cell analysis by pyrolysis-mass spectrometry (Py-MS). A numerical taxonomic approach was used with the results of conventional tests and the grouping obtained was compared with that obtained by Py-MS. All the ulcer and soft-tissue isolates and the urethritis isolates were oxidaseand urease-positive and formed a homogeneous set consistent with the reference strain of B. ureolyticus. The dental isolates differed from B. ureolyticus strains and were heterogeneous amongst themselves. None corresponded with the reference strains of B. gracilis or W . recta. The conventional and Py-MS approaches to c haracterisation produced similar groupings and each distinguished between a single cluster of ulcer-urethritis strains and several clusters of dental strains, although the dendrograms derived from the two approaches differed in the order of the clusters; in the Py-MS dendrogram one subcluster of four dental strains came within the main ulcer-urethritis cluster and a cluster of five ulcer strains was separated as a distinct group.

show abstract

“…Certain species of black-pigmented oral Bacteroides are suspected to be major causative agents of adult-type periodontitis, the most prevalent type of periodontal disease in the elderly. Among these, Bacteroides gingivalis and B. intermedius have been considered highly important in the pathogenesis of advancing periodontitis (Duerden et al, 1987;Mayrand & Holt, 1988;Slots & Listgarten, 1988). The major habitat for these bacterial species in the oral cavity is the subgingival crevice area.…”

Section: Introductionmentioning

confidence: 99%

Serological Properties and Immunobiological Activities of Lipopolysaccharides from Black-pigmented and Related Oral Bacteroides Species

Fujiwara

Nishihara²,

Kinoshita³

et al. 1988

Microbiology

View full text Add to dashboard Cite

Lipopolysaccharides (LPS) from five species of oral Bacteroides, B. gingivalis strains 38 1 and ATCC 33277, B. oralis ATCC 33269, B. 1oescheiiATCC 15930, B. intermedius ATCC 2561 1 and B. corporis ATCC 33547, were extracted from whole cells by the phenol/water procedure, and subsequently purified by treatment with nuclease and ultracentrifugation. The LPS were composed of hexoses, glucosamine, fatty acids and phosphorus. Heptose and 2-keto-3-deoxyoctonate were not detected. The LPS preparations from B. gingivalis strains 381 and ATCC 33277 presented very similar SDS-polyacrylamide gel electrophoresis patterns when stained with ammoniacal silver. They produced a fused precipitin band against an antiserum to B. gingivalis 381 LPS in immunodiffusion tests. Antisera raised against the LPS from B. loescheii and B. intermedius reacted with the LPS prepared from all the oral Bacteroides strains except those of B. gingivalis. All the LPS preparations were mitogenic for spleen cells of BALB/c (nulnu) mice, but not for thymus cells from C3H/HeN mice. The LPS induced marked mitogenic responses and polyclonal B cell activation for spleen cells of not only C3H/HeN (LPS responder) mice, but also C3H/HeJ (LPS nonresponder) mice. The mitogenic responses were not suppressed significantly upon addition of polymyxin B to the reaction mixture. These LPS also enhanced interleukin-1 production by murine peritoneal macrophages and mouse cell line 5744

show abstract

Identification of Bacteroides species from adult periodontal disease

Cited by 31 publications

References 25 publications

Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria

Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria

A Comparison of Bacteroides ureolyticus isolates from different clinical sources

Serological Properties and Immunobiological Activities of Lipopolysaccharides from Black-pigmented and Related Oral Bacteroides Species

Contact Info

Product

Resources

About