Serological Properties and Immunobiological Activities of Lipopolysaccharides from Black-pigmented and Related Oral Bacteroides Species

Abstract

Lipopolysaccharides (LPS) from five species of oral Bacteroides, B. gingivalis strains 38 1 and ATCC 33277, B. oralis ATCC 33269, B. 1oescheiiATCC 15930, B. intermedius ATCC 2561 1 and B. corporis ATCC 33547, were extracted from whole cells by the phenol/water procedure, and subsequently purified by treatment with nuclease and ultracentrifugation. The LPS were composed of hexoses, glucosamine, fatty acids and phosphorus. Heptose and 2-keto-3-deoxyoctonate were not detected. The LPS preparations from B. gingivalis strains 381 and ATCC 33277 presented very similar SDS-polyacrylamide gel electrophoresis patterns when stained with ammoniacal silver. They produced a fused precipitin band against an antiserum to B. gingivalis 381 LPS in immunodiffusion tests. Antisera raised against the LPS from B. loescheii and B. intermedius reacted with the LPS prepared from all the oral Bacteroides strains except those of B. gingivalis. All the LPS preparations were mitogenic for spleen cells of BALB/c (nulnu) mice, but not for thymus cells from C3H/HeN mice. The LPS induced marked mitogenic responses and polyclonal B cell activation for spleen cells of not only C3H/HeN (LPS responder) mice, but also C3H/HeJ (LPS nonresponder) mice. The mitogenic responses were not suppressed significantly upon addition of polymyxin B to the reaction mixture. These LPS also enhanced interleukin-1 production by murine peritoneal macrophages and mouse cell line 5744

The role of the cell‐mediated immune response to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in periodontitis

“…gingiuulis 381 promotes macrophage secretion of interleukin-1 in lipopolysaccharide-responder macrophages (isolated from lipopolysaccharide-responder C3H/HeN mice). The secretory response from lipopolysaccharide-responder macrophages was seven-fold higher than that observed for low-responder macrophages isolated from C3H/HeJ mice (54). Consistent with most reports described above, interleukin-1 synthesis induced by l?…”

“…Consistent with most reports described above, interleukin-1 synthesis induced by l? gingiuulis lipopolysaccharide in C3H/HeN macrophages was four times lower than that produced with an equivalent level of E. coli lipopolysaccharide (54). Bramanti et al examined the effect of lipopolysaccharide isolates from selected strains of l?…”

Bone resorption and local interleukin‐1α and interleukin‐1β synthesis induced by Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis lipopolysaccharide

“…The production of (1), ®broblasts (2), macrophages (8), bone marrow cells (26) and human whole blood (27) is stimulated by A. actinomycetemcomitans LPS. P. gingivalis LPS also induces IL-1 release from macrophages (4,7,28,29) and ®broblasts (30). Some of these results indicated that the levels of IL-1 induced by A. actinomycetemcomitans and E. coli LPS are higher than those induced by P. gingivalis LPS in vitro (1,2,4,5).…”

Destructive Periodontitis Lesions are Determined by the Nature of the Lymphocytic Response

“…Early studies demonstrated that LPS from P. gingivalis strains induced marked mitogenic responses and polyclonal B-cell activation in spleen cells, activated both neutrophils and macrophages, and enhanced IL-1 production by murine peritoneal macrophages (Fujiwara et al ., 1988;Isogai et al ., 1988). However, Kesavalu et al .…”