A Comparison of Bacteroides ureolyticus isolates from different clinical sources

Abstract

Summary. Clinical isolates of corroding, gram-negative, anaerobic bacilli (provisionally identified as Bacteroides ureolyticus) from superficial ulcers and soft tissue infections (1 9, non-gonococcal, non-chlamydia1 urethritis (1 2) and adult periodontal disease (14) were compared with reference strains of B. ureolyticus, B. gracilis and Wolinella recta in a series of conventional tests of morphology, biochemical activity, tolerance of dyes and bile salts, and antibiotic sensitivity, gas-liquid chromatographic analysis of metabolic products, and in whole-cell analysis by pyrolysis-mass spectrometry (Py-MS). A numerical taxonomic approach was used with the results of conventional tests and the grouping obtained was compared with that obtained by Py-MS. All the ulcer and soft-tissue isolates and the urethritis isolates were oxidaseand urease-positive and formed a homogeneous set consistent with the reference strain of B. ureolyticus. The dental isolates differed from B. ureolyticus strains and were heterogeneous amongst themselves. None corresponded with the reference strains of B. gracilis or W . recta. The conventional and Py-MS approaches to c haracterisation produced similar groupings and each distinguished between a single cluster of ulcer-urethritis strains and several clusters of dental strains, although the dendrograms derived from the two approaches differed in the order of the clusters; in the Py-MS dendrogram one subcluster of four dental strains came within the main ulcer-urethritis cluster and a cluster of five ulcer strains was separated as a distinct group.

Detection of Campylobacter concisus and Other Campylobacter Species in Colonic Biopsies from Adults with Ulcerative Colitis

“…In its previous nomenclature as Bacteroides ureolyticus it was known to cause soft tissue infections and urethritis and it has only recently been implicated as a cause of diarrhoea [22], [35], [36]. Its significant association with UC in our study suggests that these patients may be susceptible to colonisation with Campylobacter and perhaps this is genus-specific rather than to any particular Campylobacter species.…”

Routine identification of Campylobacter jejuni and Campylobacter coli from human stool samples

“…from human samples is required for epidemiological purposes. The integration of PCR methods in routine protocols of diagnostic laboratories as well as the availability of advanced phenotypic tests like the analysis of cellular fatty acid pro¢les [7] or pyrolysis mass spectrometry [8] therefore are promising attempts. This study was performed to develop a rapid, easy to perform, and reliable protocol for the correct identi¢cation of C. jejuni and C. coli isolates from human stool samples.…”

Pathogenic Potential of Campylobacter ureolyticus

“…The bacteria has also been reported to be present in amniotic fluid and the genital tract of humans (5)(6)(7)(8). In addition, C. ureolyticus has commonly been isolated from patients suffering from periodontal disease, a disease which involves inflammatory and destructive conditions of the tissues surrounding the teeth (6).…”