Identification of archaeal rDNA from subgingival dental plaque by PCR amplification and sequence analysis

Kulik, Eva M.

doi:10.1016/s0378-1097(01)00051-9

Cited by 42 publications

(88 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although Archaea have been identified or isolated among members of the human microbiota (Miller & Wolin, 1982;Belay et al, 1990;Kulik et al, 2001), it remains unclear if they actively cause any human or animal disease. A more recent study demonstrated an association between severity of human periodontal disease and the relative abundance of methanogenic Archaea (Lepp et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Microbial diversity in bovine papillomatous digital dermatitis in Holstein dairy cows from upstate New York

Santos

Pereira

Caixeta

et al. 2011

FEMS Microbiol Ecol

View full text Add to dashboard Cite

Papillomatous digital dermatitis (PDD) is one of the most prevalent diseases of cattle, adversely affecting the dairy industry by its negative effect on milk production and reproductive performance. Our objective was to use culture-independent methods to determine the microbial diversity in different strata of PDD lesions of three Holstein dairy cows, analyzing whether major differences exist compared to foot skin of three non-infected cows. Both group-specific 16S rRNA gene PCR-denaturing gradient gel electrophoresis and clone library sequencing of broad-range 16S rRNA gene showed differences between the microbial composition of healthy dairy cows and the different strata of the lesion. The predominant bacterial community in the lesion, regardless of the stratum, consisted of 166 specific phylotypes belonging to seven bacterial phyla. Spirochetes (particularly, treponemes) was the most prominent group detected in PDD deep biopsies and was only found in samples from the lesion. Additionally, one phylotype phylogenetically affiliated with uncultured Euryarchaeota was detected in two strata of the lesion. Sequences from healthy foot skin samples revealed 86 specific phylotypes that were affiliated with Firmicutes and Proteobacteria. Our study corroborates the theory that treponemes are involved in PDD disease etiology and suggests, for the first time, the presence of archaeal members in this particular bovine infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Microbial diversity in bovine papillomatous digital dermatitis in Holstein dairy cows from upstate New York

Santos

Pereira

Caixeta

et al. 2011

FEMS Microbiol Ecol

View full text Add to dashboard Cite

show abstract

“…In a more recent study, methanogens of the genus Methanobrevibacter were found in 37 of 48 cases of periodontal disease (Kulik et al, 2001). Methanogens were detected by means of a PCR assay for small subunit ribosomal DNA genes on pooled subgingival plaque samples.…”

Section: Article In Pressmentioning

confidence: 99%

Methanogenic archaea in health and disease: A novel paradigm of microbial pathogenesis

Macario

2009

International Journal of Medical Microbiology

120

View full text Add to dashboard Cite

“…Within the human body, however, only a few Euryarchaeota that belong to the methane-producing archaea have been described. Methanogens have been isolated from the human oral cavity, gut, and vagina Belay et al, 1990;Kulik et al, 2001). These archaea use H2 to reduce CO2 to CH4.…”

Section: (A) (B) (C)mentioning

confidence: 99%

Bacterial, archaeal, and eukaryal diversity in the intestines of Korean people

et al. 2008

View full text Add to dashboard Cite

The bacterial, archaeal, and eukaryal diversity in fecal samples from ten Koreans were analyzed and compared by using the PCR-fingerprinting method, denaturing gradient gel electrophoresis (DGGE). The bacteria all belonged to the Firmicutes and Bacteroidetes phyla, which were known to be the dominant bacterial species in the human intestine. Most of the archaeal sequences belonged to the methane-producing archaea but several halophilic archarea-related sequences were also detected unexpectedly. While a small number of eukaryal sequences were also detected upon DGGE analysis, these sequences were related to fungi and stramenopiles (Blastocystis hominis). With regard to the bacterial and archaeal DGGE analysis, all ten samples had one and two prominent bands, respectively, but many individual-specific bands were also observed. However, only five of the ten samples had small eukaryal DGGE bands and none of these bands was observed in all five samples. Unweighted pair group method and arithmetic averages clustering algorithm (UPGMA) clustering analysis revealed that the archaeal and bacterial communities in the ten samples had relatively higher relatedness (the average Dice coefficient values were 68.9 and 59.2% for archaea and bacteria, respectively) but the eukaryal community showed low relatedness (39.6%).

show abstract

Identification of archaeal rDNA from subgingival dental plaque by PCR amplification and sequence analysis

Cited by 42 publications

References 17 publications

Microbial diversity in bovine papillomatous digital dermatitis in Holstein dairy cows from upstate New York

Microbial diversity in bovine papillomatous digital dermatitis in Holstein dairy cows from upstate New York

Methanogenic archaea in health and disease: A novel paradigm of microbial pathogenesis

Bacterial, archaeal, and eukaryal diversity in the intestines of Korean people

Contact Info

Product

Resources

About