Identification of Anticoagulant Components in Korean Red Ginseng

Abstract

In this study, the anticoagulant compounds in Korean red ginseng (KRG) were investigated. KRG powder was extracted using hot methanol, and the methanol extract was fractionated into n-hexane, ethylacetate, n-butanol, and aqueous fractions by solvent partitioning. The remains from the methanol extraction were further extracted with water and then dialyzed to obtain low and high molecular weight fractions. The anticoagulant activities of the seven fractions were evaluated in terms of thrombin time, prothrombin time, and activated partial thromboplastin time. Among these fractions, the ethylacetate fraction showed the most potent anticoagulant activity. The active components in the ethylacetate fraction were identified as the phenolic compounds vanillic, caffeic, ferulic, and p-coumaric acid via TLC and HPLC. These findings suggest that the anticoagulant activities of phenolic compounds contribute to the cardiovascular effects of KRG.

Composition and Biological Properties of Agaricus bisporus Fruiting Bodies- a Review

“…They are characterized by antioxidant, antibacterial, antiviral, antifungal, anti-infl ammatory, and gastric-secretion stimulatory actions, documented by in vitro and in vivo studies [Czapski, 2005;Labus et al, 2011;Liu et al, 2013]. In addition, protocatechuic acid has been shown to possess immunomodulating, spasmolytic, cardioprotectant, anticoagulant, and chemopreventive properties [Wee, 2010]. The contents of phenolic compounds in fruiting bodies of A. bisporus range from 2.31 mg/kg D.W. for p-coumaric acid to 2729 mg/kg D.W. for myricetin [Czapski, 2005;Labus et al, 2011;Liu et al, 2013].…”

Anti-inflammatory activity of ethanol extract derived from Phaseolus angularis beans

“…Phytochemical characteristics of Pa-EE and standard compound quercetin were identified by high performance liquid chromatography (HPLC) analysis (Wee et al, 2010); the system was equipped with KNAUER (Wellchrom HPLC-pump, K-1001, Wellchrom fast scanning spectrophotometer K-2600, and 4 channel degasser K-500). Elution solvents were buffer A (0.1% TFA in H 2 O) and buffer B (0.08% TFA in 95% ACN + 5% H 2 O).…”

Inhibitory Effects of Total Saponin Korean Red Ginseng on Thromboxane A2Production and P-Selectin ExpressionviaSuppressing Mitogen-Activated Protein Kinases

“…Recently, it is reported that KRG-TS has an effect on CVD through reduction of blood pressure (Chung, 2010), anti-coagulation effects (Wee et al, 2010), endothelium relaxation (Jung et al, 2011), and inhibition of hypercholesterolemia-induced platelet aggregation (Hwang et al, 2008). In our previous report, we demonstrated that KRG-TS was involved in increase of cAMP level and subsequent reduction of [Ca 2+ ] i mobilization in thrombin-induced rat platelet aggregation (Lee et al, 2013).…”