Identification of an Intestinal Immune Response Using Peripheral Blood Lymphocytes

Forrest, B. D.

doi:10.1016/s0140-6736(88)90284-x

Cited by 121 publications

(58 citation statements)

References 13 publications

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“…According to a recent report (1), interaction with indigenous intestinal microbes induces protective mucosal IgA responses in a murine model, which was interpreted to ensure tolerance to commensal bacteria. The ELISPOT method applied in this study reliably reflects mucosal immune responsiveness (15,18) and thus our results may extend this notion of the role of intestinal microbial stimulation to establishing tolerance to dietary antigens.…”

Section: Discussionsupporting

confidence: 63%

Specific Probiotics in Enhancing Maturation of IgA Responses in Formula-Fed Infants

Arvilommi²,

2006

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ABSTRACT:The first months of life represent a critical period for the maturation of the infant's immune system and, thus, a window of opportunity for measures to reduce the risk of disease. We hypothesized that specific probiotics might promote mucosal immunologic maturation in formula-fed infants. The numbers of cow's milkspecific and total IgA-secreting cells were measured at 3, 7, and 12 mo of age in a double-blind placebo-controlled study of 72 infants with early artificial feeding. The infants consumed infant formula supplemented with specific probiotics (Lactobacillus GG and Bifidobacterium lactis

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Section: Discussionsupporting

confidence: 63%

Specific Probiotics in Enhancing Maturation of IgA Responses in Formula-Fed Infants

Arvilommi²,

2006

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“…The total number of ISC (antigen-nonspecific immune response) and sASC (antigen-specific immune response) were determined by the ELlSPOT method, as described previously (8-10). We and others have shown that the number of these lymphocytes in peripheral blood reaches a maximum 6-8 dafter oral administration of antigen (8)(9)(10)(11)(12).…”

Section: Resultsmentioning

confidence: 99%

“…After initia l contact with the antigen in the gut, these lymphocytes cycle through the peripheral blood to localize back to the intestine. It has been shown not only in experimental animals but also in humans that the cells appear in blood a week after anti gen administration (10)(11)(12)15). In addition, it has been demon strated that th e lymphocyte response can be measured in blood more consistently than the antibody response in either duodenal fluid or seru m (10).…”

Section: Resultsmentioning

confidence: 99%

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Enhancement of the Circulating Antibody Secreting Cell Response in Human Diarrhea by a Human Lactobacillus Strain

et al. 1992

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MATER IALS AND METHODScourse of diarrh ea. Maln utrition has been report ed to increase the absorption of potentially harmful antigens (2) and to impai r imm une responses (3). A poor nutrition al state may thu s increase the risk of protracted diarrh ea as well as enhance susceptibility to other infectio ns and to gastroi ntestinal allergy. The nutritio nal state of even well-no urished infants deteriorat es rapidly during diarrhea, either because of loss of appetite, deliberate withholding of oral feeds, or partial malabsorption caused by viral invasio n ofenterocytes (4).Rapid reintrodu ction of oral feedings after rehydration has been advocated to coun terac t the pote nti al hazards related to fasting during diarrhea (5). We have previously shown that rapid refeeding results in earlier cessatio n of diarrhea in well-nourished children; also, cow milk produ cts are tolerated (6). A furt her shortening of diarrhea resulted from administratio n of hu man Lactobacillus stra in (Lactobacillus GG, Gefilac, Valio Finni sh Co-operative Dairies' Associatio n, Helsinki, Finland ) together with the rapid refeeding schedule (7). The mechanisms behind such a favorable outc ome remain poorly understood. Th e effect of nutritional therapy may be immunologically med iated and may prove important in eradicating enteric infections in the imm unocompromised host.The objective of the present study was to evaluate the effect of L actobacillus GG on the intestinal immune response triggered by rotavirus infection in well-nourished children. For th is pu rpose, we used the ELISPOT assay, which measures ISC and sASC amo ng circulating lymphocytes. T hese cells are arrested du ring their maturation cycle in peripheral blood, giving indirect evidence of gut local im m une respo nse (8-10).Patients. Forty-four well-no urished children (33.4% female), between 7 and 37 mo of age, were enro lled in the present study. Th ey were admitted for acute gastroen teritis of less than 7 d du ration at the Departm ent of Pediatrics, Ta mpere U niversity Hospital, during a rotavirus epidemic .Informed consent was obtaine d from the patients' pare nts, and the protocol was approved by the hospital's Committee on Ethical Practice.Ma nagement and samples. U pon ad mission, the children were weighed and clinically examin ed. The severity of dehydration (%) was estimated. Th e children were treated according to sta ndard practice: oral rehyd ration was accom plished in 6 h with a , solution containing Na" 60 mmo l/ L and glucose 144 mm ol/L (Osmosal Novum, Leiras, Turku , Finland) given at twice the estimated fluid loss with a minimum of 30 mL/kg body weight. Th e patients were weighed daily. Th e attending nurs es followed the quality (characterized as watery, loose, or solid) and number of stools and vomitus. The du ration of diarrh ea was counted 14 1

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“…It is a new immunoassay that is thought to give indirect evidence of the gut immunologic Clinical data. As shown in Figure 1, weight gain during the events (27)(28)(29). The method is based on the maturation cycle of experimental feeding period was comparable in the study groups.…”

Section: Controlsmentioning

confidence: 90%

Diet during Rotavirus Enteritis Affects Jejunal Permeability to Macromolecules in Suckling Rats

et al. 1993

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Identification of an Intestinal Immune Response Using Peripheral Blood Lymphocytes

Cited by 121 publications

References 13 publications

Specific Probiotics in Enhancing Maturation of IgA Responses in Formula-Fed Infants

Specific Probiotics in Enhancing Maturation of IgA Responses in Formula-Fed Infants

Enhancement of the Circulating Antibody Secreting Cell Response in Human Diarrhea by a Human Lactobacillus Strain

Diet during Rotavirus Enteritis Affects Jejunal Permeability to Macromolecules in Suckling Rats

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