Identification of an Antagonistic Probiotic Combination Protecting Ornate Spiny Lobster (Panulirus ornatus) Larvae against Vibrio owensii Infection

Goulden, Evan F.; Hall, Michael R.; Pereg, Lily; Høj, Lone

doi:10.1371/journal.pone.0039667

Cited by 32 publications

(25 citation statements)

References 52 publications

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“…While addition of probionts to Artemia cultures has previously shown to reduce pathogenic Vibrio spp. populations (Verschuere et al ., ; Villamil et al ., ), the present study showed that ecological niche specialization by two probionts likely contributed to their additive protective effect against V. owensii DY05 that was observed in an earlier study (Goulden et al ., ). The Pseudoalteromonas probiont PP107 was localized only on external surfaces of the vector organism ( Artemia ) and phyllosomas, while the Vibrio probiont PP05 was primarily localized in their respective digestive systems.…”

Section: Discussionmentioning

confidence: 97%

“…Ambient planktonic pathogen cells in the Artemia cultures were reduced in the presence of probionts compared with pathogen‐only controls, confirming that the probionts can inhibit planktonic growth of V. owensii DY05 (Goulden et al ., ). Our previous study showed that planktonic growth of V. owensii DY05 could be inhibited by either of the probiotic strains; however, PP107 was found to be more efficient at low densities (Goulden et al ., ). In the Artemia gut, the pathogen population was significantly reduced from high‐density bioaccumulations in pathogen‐only controls to isolated cells in Artemia enriched with pathogen and probionts, and spread plating confirmed a reduced pathogen load.…”

Section: Discussionmentioning

confidence: 99%

“…While Pseudoalteromonas sp. PP107 was isolated from arrow worms ( Chaetognatha ), a natural prey item of phyllosomas, we have isolated closely related Pseudoalteromonas strains from both wild and aquaculture‐reared phyllosomas (Goulden et al ., ). It is uncertain whether pseudoalteromonads associated with phyllosomas simply use a hitchhiking strategy to facilitate their dispersal (Grossart et al ., ) or whether they are true ectosymbionts.…”

Section: Discussionmentioning

confidence: 99%

“…To confirm the virulence of V. owensii DY05[RFP] and the probiotic protective benefits of PP05[GFP], PP05[RFP] and PP107[GFP], stage 1 (2 day old) P. ornatus phyllosomas were experimentally infected using vectored challenge via instar II Artemia nauplii (Goulden et al ., ,b). Strain combinations were selected to correspond with the subsequent studies described below.…”

Section: Methodsmentioning

confidence: 99%

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Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae

Goulden

Hall

Pereg

et al. 2012

Environ Microbiol Rep

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The development of efficient probiotic application protocols for use in marine larviculture relies on comprehensive understanding of pathogen-probiont-host interactions. The probiont combination of Pseudoalteromonas sp. PP107 and Vibrio sp. PP05 provides additive protection against vectored Vibrio owensii DY05 infection in larvae (phyllosomas) of ornate spiny lobster, Panulirus ornatus. Here, fluorescently tagged strains were used to demonstrate niche specialization of these probionts in both the live feed vector organism Artemia and in phyllosomas. The pathogen was vulnerable to direct interaction with PP05 in the bacterioplankton as well as in the Artemia gut and the phyllosoma foregut and midgut gland. In contrast, PP107 was localized on external surfaces of Artemia and phyllosomas, and direct interaction with the pathogen was limited to the bacterioplankton. While PP107 was the overall dominant ectobiont on the phyllosoma cephalothorax and inner leg segments, PP05 was the primary colonizer of outer leg segments, nutrient-rich locales that may promote ingestion during feeding. This study shows that niche specialization can contribute to the additive probiotic effect of a probiotic mixture and highlights that probiotic enrichment of Artemia cultures can intercept the infection cycle of V. owensii DY05 in early-stage P. ornatus phyllosomas.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae

Goulden

Hall

Pereg

et al. 2012

Environ Microbiol Rep

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“…This study revealed that L. plantarum has the capacity to inhibit S. parasitica and that it would be possible to design new biocontrol of this pathogen in catfish. Moreover, Goulden et al (2012) evaluated the potential of multi-species probiotic treatment and demonstrated that natural microbial communities associated with wild phyllosomas and zooplankton prey support antagonistic bacteria capable of in vivo suppression of a pathogen causing epizootics in phyllosoma culture systems. Ali et al (2013) isolated probiotic strains from different fermented cheese products, which have been reported to show antifungal activity against the plant pathogens Rhizoctonia solani and Fusarium oxysporum.…”

Section: Antifungalmentioning

confidence: 99%

Application of Probiotics in Shrimp Aquaculture: Importance, Mechanisms of Action, and Methods of Administration

Kumar

Roy

Meena

et al. 2016

Reviews in Fisheries Science & Aquaculture

139

105

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Shrimp aquaculture is the fastest growing animal food producing sectors in the world due to its high demand in developed countries; however, they are affected by diseases mainly caused by opportunistic pathogens results in huge economic losses. Today preventive and management measures are central concern to overcome such outbreak of diseases. The adverse effect of chemotherapeutics in shrimp farming has drawn attention for the development of non-antibiotic and environment friendly agents, which is one of the key factors for health management in shrimp aquaculture. In recent years, application of probiotics against virus and bacteria in shrimp farming had been a novel and safe approach since they promote the innate immune response. Probiotics for bacterial diseases like vibriosis is well reported, but for viral diseases, the authentic strains still need research. Apart from the discovery of new or better formulations, improvement in the probiotic benefits may be helpful. Thus, better and economic production methods, administration ways or combination with other preventive/therapeutic measures are welcomed. Further studies are still necessary to increase the knowledge about use of probiotics to control bacterial infections in shrimp but much more efforts are needed in the case of viral diseases. In this review, potential application of probiotics in shrimp aquaculture: importance, mechanisms of action and methods of administration are being focused. The article also explains the limitations and prospects of probiotics in sustainable and ecofriendly shrimp culture to augment the total shrimp aquaculture production.

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Probiotics in fish and shellfish culture: immunomodulatory and ecophysiological responses

Meena

Behera

et al. 2014

Fish Physiol Biochem

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Aquaculture is emerging as one of the most viable and promising enterprises for keeping pace with the surging need for animal protein, providing nutritional and food security to humans, particularly those residing in regions where livestock is relatively scarce. With every step toward intensification of aquaculture practices, there is an increase in the stress level in the animal as well as the environment. Hence, disease outbreak is being increasingly recognized as one of the most important constraints to aquaculture production in many countries, including India. Conventionally, the disease control in aquaculture has relied on the use of chemical compounds and antibiotics. The development of non-antibiotic and environmentally friendly agents is one of the key factors for health management in aquaculture. Consequently, with the emerging need for environmentally friendly aquaculture, the use of alternatives to antibiotic growth promoters in fish nutrition is now widely accepted. In recent years, probiotics have taken center stage and are being used as an unconventional approach that has numerous beneficial effects in fish and shellfish culture: improved activity of gastrointestinal microbiota and enhanced immune status, disease resistance, survival, feed utilization and growth performance. As natural products, probiotics have much potential to increase the efficiency and sustainability of aquaculture production. Therefore, comprehensive research to fully characterize the intestinal microbiota of prominent fish species, mechanisms of action of probiotics and their effects on the intestinal ecosystem, immunity, fish health and performance is reasonable. This review highlights the classifications and applications of probiotics in aquaculture. The review also summarizes the advancement and research highlights of the probiotic status and mode of action, which are of great significance from an ecofriendly, sustainable, intensive aquaculture point of view.

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Identification of an Antagonistic Probiotic Combination Protecting Ornate Spiny Lobster (Panulirus ornatus) Larvae against Vibrio owensii Infection

Cited by 32 publications

References 52 publications

Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae

Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae

Application of Probiotics in Shrimp Aquaculture: Importance, Mechanisms of Action, and Methods of Administration

Probiotics in fish and shellfish culture: immunomodulatory and ecophysiological responses

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