Identification of a cellulose synthase-associated protein required for cellulose biosynthesis

Gu, Ying; Kaplinsky, Nicholas J.; Bringmann, Martin; Cobb, Ale×ander R.; Carroll, Andrew; Sampathkumar, Arun; Baskin, Tobias I.; Persson, Staffan; Somerville, Chris

doi:10.1073/pnas.1007092107

Cited by 228 publications

(252 citation statements)

References 32 publications

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“…Rather, the movement of CESA particles was proposed to be a function of a polymerization force generated by the translocating glucan chain (Diotallevi and Mulder, 2007). The PM movement of CESA particles in untreated cells was bidirectional with an average velocity of 336 6 167 nm min 21 , which is consistent with numerous prior studies (Paredez et al, 2006;Bischoff et al, 2009;Crowell et al, 2009;Gutierrez et al, 2009;Gu et al, 2010;Li et al, 2012). After treatment with indaziflam, YFP:CESA6 velocity was reduced to 119 6 95 nm min 21 (Fig.…”

Section: Isoxaben-and Quinoxyphen-resistant Plants Are Not Cross Resisupporting

confidence: 79%

“…Reduced CESA Velocity after Indaziflam Treatment Is CSI1 Independent CSI1 was identified as a primary linker protein between MTs and CSCs (Gu et al, 2010;Bringmann et al, 2012;Lei et al, 2012;Li et al, 2012). In csi1 mutants, CESA particles in the PM were found to display reduced velocity and their association with MTs was completely disrupted (Gu et al, 2010;Li et al, 2012).…”

Section: Isoxaben-and Quinoxyphen-resistant Plants Are Not Cross Resimentioning

confidence: 99%

“…Resistance screens to CBIs have uncovered key genes in cellulose biosynthesis (Scheible et al, 2001;Desprez et al, 2002). Furthermore, CBIs such as isoxaben have also been effective in linking accessory proteins with CESAs in the CSC (Robert et al, 2005;Gu et al, 2010). Therefore, it is important to extend our range of CBI compounds.…”

mentioning

confidence: 99%

“…In plants, the CSC, visualized with freeze fracture microscopy, is a solitary, hexagonal rosette-shaped complex (Herth and Weber, 1984;Delmer, 1999) and at least three of the catalytic CELLULOSE SYNTHASE A (CESA) proteins are required in each CSC for the production of cellulose (Desprez et al, 2007;Persson et al, 2007). In addition to CESAs, several accessory proteins have been discovered to be necessary for the production and deposition of cellulose, such as KORRIGAN (Lane et al, 2001), COBRA (Roudier et al, 2005) and CELLULOSE SYNTHASE INTERACTING1 (CSI1; Gu et al, 2010), as well as several others that are yet to be identified. The loss of function in any of the aforementioned proteins causes complete or partial loss of anisotropic growth in cells undergoing expansion, resulting in radial swelling.…”

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confidence: 99%

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Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

Brabham¹,

Lei

et al. 2014

Plant Physiology

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Cellulose biosynthesis is a common feature of land plants. Therefore, cellulose biosynthesis inhibitors (CBIs) have a potentially broad-acting herbicidal mode of action and are also useful tools in decoding fundamental aspects of cellulose biosynthesis. Here, we characterize the herbicide indaziflam as a CBI and provide insight into its inhibitory mechanism. Indaziflam-treated seedlings exhibited the CBI-like symptomologies of radial swelling and ectopic lignification. Furthermore, indaziflam inhibited the production of cellulose within ,1 h of treatment and in a dose-dependent manner. Unlike the CBI isoxaben, indaziflam had strong CBI activity in both a monocotylonous plant (Poa annua) and a dicotyledonous plant (Arabidopsis [Arabidopsis thaliana]). Arabidopsis mutants resistant to known CBIs isoxaben or quinoxyphen were not cross resistant to indaziflam, suggesting a different molecular target for indaziflam. To explore this further, we monitored the distribution and mobility of fluorescently labeled CELLULOSE SYNTHASE A (CESA) proteins in living cells of Arabidopsis during indaziflam exposure. Indaziflam caused a reduction in the velocity of YELLOW FLUORESCENT PROTEIN:CESA6 particles at the plasma membrane focal plane compared with controls. Microtubule morphology and motility were not altered after indaziflam treatment. In the hypocotyl expansion zone, indaziflam caused an atypical increase in the density of plasma membrane-localized CESA particles. Interestingly, this was accompanied by a cellulose synthase interacting1-independent reduction in the normal coincidence rate between microtubules and CESA particles. As a CBI, for which there is little evidence of evolved weed resistance, indaziflam represents an important addition to the action mechanisms available for weed management.

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Section: Isoxaben-and Quinoxyphen-resistant Plants Are Not Cross Resisupporting

confidence: 79%

Section: Isoxaben-and Quinoxyphen-resistant Plants Are Not Cross Resimentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

Brabham¹,

Lei

et al. 2014

Plant Physiology

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“…Cellulose synthase coaligns with and moves bidirectionally along cortical microtubules (Paredez et al, 2006). A large cellulose synthase interacting protein (CSI1) links microtubule and CESA complexes (Gu et al, 2010), and two companion proteins of cellulose synthase (CC1 and CC2) bind to microtubules and promote microtubule dynamics (Endler et al, 2015).…”

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confidence: 99%

Anisotropic Cell Expansion Is Affected through the Bidirectional Mobility of Cellulose Synthase Complexes and Phosphorylation at Two Critical Residues on CESA3

Chen

Jia²,

Zhao³

et al. 2016

Plant Physiol.

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Here we report that phosphorylation status of S211 and T212 of the CESA3 component of Arabidopsis (Arabidopsis thaliana) cellulose synthase impacts the regulation of anisotropic cell expansion as well as cellulose synthesis and deposition and microtubule-dependent bidirectional mobility of CESA complexes. Mutation of S211 to Ala caused a significant decrease in the length of etiolated hypocotyls and primary roots, while root hairs were not significantly affected. By contrast, the S211E mutation stunted the growth of root hairs, but primary roots were not significantly affected. Similarly, T212E caused a decrease in the length of root hairs but not root length. However, T212E stunted the growth of etiolated hypocotyls. Live-cell imaging of fluorescently labeled CESA showed that the rate of movement of CESA particles was directionally asymmetric in etiolated hypocotyls of S211A and T212E mutants, while similar bidirectional velocities were observed with the wild-type control and S211E and T212A mutant lines. Analysis of cell wall composition and the innermost layer of cell wall suggests a role for phosphorylation of CESA3 S211 and T212 in cellulose aggregation into fibrillar bundles. These results suggest that microtubule-guided bidirectional mobility of CESA complexes is fine-tuned by phosphorylation of CESA3 S211 and T212, which may, in turn, modulate cellulose synthesis and organization, resulting in or contributing to the observed defects of anisotropic cell expansion.

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Cellulose

French

Pérez

Bulone

et al. 2018

Encyclopedia of Polymer Science and Technology

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This article covers nomenclature, sources, biosynthesis, preparation, uses, microcrystalline cellulose, structural chemistry, reactions, solvents, and liquid crystals. Cellulose for commercial purposes comes mostly from wood and cotton, whereas cellulose for research comes from bacteria, algae, and ramie (also a textile fiber). Preparation includes pulping and purification, with an alternative method of steam explosion. The pore structure of cellulose is mentioned. Emphasis is given to cellulose crystal structures. Cellulose solutions are important to the rayon and cellophane industries, and new solvents are of interest because they may lessen pollution and might permit commercial production of stronger cellulosic materials through the formation of liquid crystals. Common physical methods for assessment of cellulose structure are discussed. Figures include the chemical and physical structures of the molecule, sources of cellulose, biosynthesis charts, molecular weight distributions, crystallite sizes, X‐ray diffraction patterns, nuclear magnetic resonance spectra, conformational energy plots, and the unit cell structures of cellulose I–IV.

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Identification of a cellulose synthase-associated protein required for cellulose biosynthesis

Cited by 228 publications

References 32 publications

Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

Indaziflam Herbicidal Action: A Potent Cellulose Biosynthesis Inhibitor

Anisotropic Cell Expansion Is Affected through the Bidirectional Mobility of Cellulose Synthase Complexes and Phosphorylation at Two Critical Residues on CESA3

Cellulose

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