The CESA1 component of cellulose synthase is phosphorylated at sites clustered in two hypervariable regions of the protein. Mutations of the phosphorylated residues to Ala (A) or Glu (E) alter anisotropic cell expansion and cellulose synthesis in rapidly expanding roots and hypocotyls. Expression of T166E, S686E, or S688E mutants of CESA1 fully rescued the temperature sensitive cesA1-1 allele (rsw1) at a restrictive temperature whereas mutations to A at these positions caused defects in anisotropic cell expansion. However, mutations to E at residues surrounding T166 (i.e., S162, T165, and S167) caused opposite effects. Live-cell imaging of fluorescently labeled CESA showed close correlations between tissue or cell morphology and patterns of bidirectional motility of CESA complexes in the plasma membrane. In the WT, CESA complexes moved at similar velocities in both directions along microtubule tracks. By contrast, the rate of movement of CESA particles was directionally asymmetric in mutant lines that exhibited abnormal tissue or cell expansion, and the asymmetry was removed upon depolymerizing microtubules with oryzalin. This suggests that phosphorylation of CESA differentially affects a polar interaction with microtubules that may regulate the length or quantity of a subset of cellulose microfibrils and that this, in turn, alters microfibril structure in the primary cell wall resulting in or contributing to the observed defect in anisotropic cell expansion.Arabidopsis | cell wall | CESA protein | microtubule | regulation I n higher plants, cellulose is a composite polymer of long unbranched β-1,4-linked glucan chains that are hydrogen-bonded to form microfibrils of approximately 36 chains (1). The microfibrils are the primary load-bearing component of cell walls (2, 3), and defects in cellulose synthesis or organization (4) result in decreased anisotropy of cell wall expansion and an inability to achieve differentiated cell shape (2-4).Cellulose is synthesized by plasma-membrane complexes that have been visualized by freeze-fracture EM as hexagonal rosettes 25 to 30 nm in diameter (5). The complexes are thought to be composed of three functional types of structurally similar CESA proteins that are believed to be the catalytic subunits. For primary wall cellulose synthesis, CESA1 and CESA3 appear to be absolutely required whereas CESA2, CESA5, CESA6, and CESA9 are at least partially redundant (6). Dynamic visualization of CESA complexes in growing Arabidopsis cells showed that labeled (YFP:: CESA6) synthetic complexes appeared as discrete particles at the plasma membrane that move bidirectionally along linear paths coincident with underlying cortical microtubules (7). These observations, together with previous (8) and subsequent studies (9-11), suggest that cortical microtubule organization plays a role in guiding the orientation of cellulose microfibril deposition.All CESA proteins are predicted to have two transmembrane helices in the N-terminal region and six in the C-terminal region (Fig. 1). Between the tw...

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Suc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various environmental stimuli in the leaves of five dicots (Arabidopsis Extensive data on expression of SUTs in relation to changes of environmental conditions were obtained through a global analysis of 168 transcriptomics data sets. Results were validated by quantitative PCR measurements and extended by the measurement of photosynthesis rate and phloem sugar content to draw insight on the correlation of SUT expression and sugar export from leaves. For the apoplasmic phloem loaders, a clear difference in transcriptional regulation in response to different environmental stimuli was observed. The consistent patterns of SUT expression under abiotic stress indicates which types of SUTs are involved in the regulation of leaf sugar status and in stress signaling. Furthermore, it is shown that down-regulation of phloem loading is likely to be caused by transcriptional regulation of SUTs, while up-regulation depends on post-transcriptional regulation. In poplar, expression of PtaSUT4 was found to consistently respond to environmental stimuli, suggesting a significant role in the regulation of sugar export from leaves in this passive symplasmic phloem loader.

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The intensive computation of High Efficiency Video Coding (HEVC) engenders challenges for the hardwired encoder in terms of the hardware overhead and the power dissipation. On the other hand, the constrains in hardwired encoder design seriously degrade the efficiency of software oriented fast coding unit (CU) partition mode decision algorithms. A fast algorithm is attributed as VLSI friendly, when it possesses the following properties. First, the maximum complexity of encoding a coding tree unit (CTU) could be reduced. Second, the parallelism of the hardwired encoder should not be deteriorated. Third, the process engine of the fast algorithm must be of low hardware- and power-overhead. In this paper, we devise the convolution neural network based fast algorithm to decrease no less than two CU partition modes in each CTU for full rate-distortion optimization (RDO) processing, thereby reducing the encoder's hardware complexity. As our algorithm does not depend on the correlations among CU depths or spatially nearby CUs, it is friendly to the parallel processing and does not deteriorate the rhythm of RDO pipelining. Experiments illustrated that, an averaged 61.1% intraencoding time was saved, whereas the Bjøntegaard-Delta bit-rate augment is 2.67%. Capitalizing on the optimal arithmetic representation, we developed the high-speed [714 MHz in the worst conditions (125 °C, 0.9 V)] and low-cost (42.5k gate) accelerator for our fast algorithm by using TSMC 65-nm CMOS technology. One accelerator could support HD1080p at 55 frames/s real-time encoding. The corresponding power dissipation was 16.2 mW at 714 MHz. Finally, our accelerator is provided with good scalability. Four accelerators fulfill the throughput requirements of UltraHD-4K at 55 frames/s.

E7, a single domain Family 33 cellulose binding module (CBM) protein, and E8, a non-catalytic, three-domain protein consisting of a Family 33 CBM, a FNIII domain, followed by a Family 2 CBM, were cloned, expressed, purified, and characterized. Western blots showed that E7 and E8 were induced and secreted when Thermobifida fusca was grown on cellobiose, Solka floc, switchgrass, or alfalfa as well as on beta-1,3 linked glucose molecules such as laminaribiose or pachyman. E8 bound well to alpha- and beta-chitin and bacterial microcrystalline cellulose (BMCC) at all pHs tested. E7 bound strongly to beta-chitin, less well to alpha-chitin and more weakly to BMCC than E8. Filter paper binding assays showed that E7 was 28% bound, E8 was 39% bound, a purified CBM2 binding domain from Cel6B was 88% bound, and only 5% of the Cel5A catalytic domain was bound. A C-terminal 6xHis tag influenced binding of both E7 and E8 to these substrates. Filter paper activity assays showed enhanced activity of T. fusca cellulases when E7 or E8 was present. This effect was observed at very low concentrations of cellulases or at very long times into the reaction and was mainly independent of the type of cellulase and the number of cellulases in the mixture. E8, and to a lesser extent E7, significantly enhanced the activity of Serratia marscescens Chitinase C on beta-chitin.

Highlights d The permeability of plasmodesmata can be selectively regulated within one cell d Leaf epidermis cell plasmodesmata are modulated for fast diffusion along the midrib d Efficient auxin transport from leaf tip to petiole depends on the ''diffusion highway''

Summary In the sieve elements (SEs) of the phloem, carbohydrates are transported throughout the whole plant from their site of production to sites of consumption or storage. SE structure, especially of the pore‐rich end walls, has a direct effect on translocation efficiency. Differences in pore size and other features were interpreted as an evolutionary trend towards reduced hydraulic resistance. However, this has never been confirmed.Anatomical data of 447 species of woody angiosperms and gymnosperms were used for a phylogenetic analysis of end wall types, calculation of hydraulic resistance and correlation analysis with morphological and physiological variables. end wall types were defined according to pore arrangement: either grouped into a single area (simple) or into multiple areas along the end wall (compound).Convergent evolution of end wall types was demonstrated in woody angiosperms. In addition, an optimization of end wall resistance with plant height was discovered, but found to be independent of end wall type. While physiological factors also showed no correlation with end wall types, the number of sieve areas per end wall was found to scale with SE length.The results exclude the minimization of hydraulic resistance as evolutionary driver of different end wall types, contradicting this long‐standing assumption. Instead, end wall type might depend on SE length.