Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio

Molecular basis and genetic improvement of economically important traits in aquaculture animals

“…Gibel carp, because of the polyploid background and dual reproduction modes of gynogenesis and sexuality, has become a promising fish for screening and isolating reproduction-related genes that are involved in primordial germ cell formation, oocyte maturation and egg fertilization. Using suppression subtractive hybridization and protein purification [51,52], some important genes, such as germ plasm markers vasa [53] and Dazl [54], oocyte maturation-related factors cyclin A2 [55], C-type lectin [52], C1q-like [56][57][58], histone H2A variant h2af1o [59], and maternal-effect factor spindlin [60], have been identified and functionally characterized from gibel carp. For example, Sun et al [61] have observed oocytespecific expression pattern and dynamic distribution of Spindlin during oocyte maturation and egg fertilization, demonstrated its association and interaction with β-tubulin and spindle, and found that its elimination destroys spindle assembly, and thereby disturbs the oocyte-to-embryo transition and first cleavage, which provided the first direct evidence for the critical oocyte-to-embryo transition function of Spindlin in vertebrates.…”

Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp

“…Protein extracts were isolated from adult tissues (heart, liver, spleen, kidney, muscle, brain, testis and ovary) of gibel carp using RIPA Lysis Buffer (Beyotime), and Western blot detection was performed according to the previous reports (Mei et al, 2008a(Mei et al, , 2008bZhu et al, 2008). β-Actin was also used as an internal control.…”

“…All these features made gibel carp to become a unique case to explore the evolution of sex determination and differentiation in vertebrates. Using gibel carp as a research model for screening reproduction-related genes (Xie et al, 2001), some important genes, such as germ plasm markers vasa (Xu et al, 2005) and Dazl (Peng et al, 2009), C-type lectin (Dong et al, 2004), C1q-like (Mei et al, 2008a(Mei et al, , 2008b(Mei et al, , 2014, histone H2A variant h2af1o (Wu et al, 2009;Yue et al, 2013), and maternal-effect factor spindlin (Sun et al, 2010), had been identified and functionally characterized (Gui and Zhu, 2012), but no sex-determination related genes had been analyzed in the polyploid gibel carp. Recently, through BAC identification and sequencing, two divergent Dmrt1 genes, CagDmrt1-1 and CagDmrt1-2, have been revealed from C. auratus gibelio , however, their molecular characterization and expression pattern, especially their protein distribution in testicular cells, are still unclear.…”

Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper

“…This sequence was then cloned into PET-32a (+) via the EcoRV and XhoI restriction enzyme sites and expressed in E. coli (DE3). To acquire the polyclonal antiserum, the expressed proteins were purified with His-tag purification kit (Novagene, USA) and applied to immunize white rabbit as described previously Mei et al, 2008a). White rabbits were first injected with complete Freund Adjuvant (Sigma) at the vola.…”