Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper

Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio

“…Then, about 1 μg of the DNase-treated total RNAs were respectively reverse-transcribed with GoldScript Reverse Transcriptase and GSP primer (Invitrogen) as described previously (Xiao et al, 2014). The fragments representing slbp2 and β-actin (as control) were respectively amplified by RT-PCR with specific primers of slbp2-F and slbp2-R, β-actin-F and β-actin-R (Table 1) as described previously (Huang et al, 2009). Briefly, the amplified parameters included pre-denaturation at 94°C for 3 min, 30 cycles and a final extension at 72°C for 5 min, and each PCR cycle included denaturation at 95°C for 40 s, annealing at 60°C for 30 s and 72°C for 30 s.…”

Molecular basis and genetic improvement of economically important traits in aquaculture animals

“…In hermaphrodite red-spotted grouper and rice field eel [83,84], the gonad-specific aromatase (cyp19a1a) had been also demonstrated to be expressed by follicular cells of follicular layer around oocytes. The promoter activity analysis showed a number of potential binding sites for some transcriptional factors, such as SOX5, GATA gene family, CREB, AP1, FOXL1, C/EBP, ARE and SF-1, and most of the motifs are conserved among the studied fish species.…”

Subcellular localization and functional characterization of a fish IRF9 from crucian carp Carassius auratus

“…At 48 h posttransfection, the transfected cells were harvested and lysed according to the Dual-Luciferase Reporter Assay System (Promega). Luciferase activities were measured by a Junior LB9509 luminometer (Berthod, Pforzhiem, Germany) and normalized to the amounts of Renilla luciferase activities as described previously [18,19]. The results were representatives of more than three independent experiments, each performed in triplicates.…”