Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio

Liu, Zhen; Zhang, Xiaojuan; Wang, Wei; Zhang, Jun; Li, Zhi; Gui, Jian‐Fang

doi:10.1016/j.cbpb.2015.08.011

Cited by 4 publications

(9 citation statements)

References 47 publications

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“…1B). Consistent with Cgslbp2 in gibel carp (Liu et al 2015), zebrafish slbp2 transcripts and proteins were detected only in unfertilized eggs and early embryos before the 50% epiboly stage (Fig. 1C), indicating that zebrafish Slbp2 is a maternal factor and no zygotic product is synthesized during embryogenesis.…”

Section: Oocyte-specific and Maternal Expression Pattern Of Zebrafishsupporting

confidence: 53%

“…1C), indicating that zebrafish Slbp2 is a maternal factor and no zygotic product is synthesized during embryogenesis. The distribution of zebrafish slbp2 in the ovary is also the same as that of Cgslbp2 in gibel carp (Liu et al 2015). Zebrafish slbp2 was specifically expressed in the oocytes, not in somatic cells surrounding oocytes (Fig.…”

Section: Oocyte-specific and Maternal Expression Pattern Of Zebrafishmentioning

confidence: 59%

“…Additionally, bovine SLBP2 and xSLBP2 display differences in their expression and localization during oogenesis (Thelie et al 2012). Although the functions of SLBP in histone mRNA metabolism have been well revealed (Marzluff and Koreski 2017), the reports about SLBP2 are very scarce (Wang et al 1999;Thelie et al 2012;Liu et al 2015) and the function of SLBP2 is still mostly unknown.…”

Section: Introductionmentioning

confidence: 99%

“…In zebrafish slbp1 mutant, retinal neurogenesis is severely delayed (Imai et al 2014). Recently, we identified oocyte-specific slbp2 from gibel carp (Carassius gibelio) (Gui and Zhou 2010;Zhou and Gui 2017) and analyzed its dynamic expression pattern during oogenesis and embryogenesis (Liu et al 2015). Imai et al (2014) also detected maternal slbp2 in early zebrafish embryos.…”

Section: Introductionmentioning

confidence: 99%

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Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis

Liu

et al. 2018

RNA

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Stem-loop binding protein (SLBP) is required for replication-dependent histone mRNA metabolism in mammals. Zebrafish possesses two , and is necessary for retinal neurogenesis. However, the detailed expression and function of in zebrafish are still unknown. In this study, we first identified zebrafish as an oocyte-specific maternal factor and then generated a maternal-zygotic F3 homozygous mutant (MZΔ4) using CRISPR/Cas9. The depletion of maternal Slbp2 disrupted early nuclear cleavage, which resulted in developmental arrest at the MBT stage. The developmental defects could be rescued in transgenic MZΔ4 embryos. However, homozygous mutant MZΔ1 developed normally, indicating is dispensable for zebrafish early embryogenesis. Through comparative proteome and transcriptome profiling between WT and MZΔ4 embryos, we identified many differentially expressed proteins and genes. In comparison with those in WT embryos, four replication-dependent histones, including H2a, H2b, H3, and H4, all reduced their expression, while histone variant significantly increased in MZΔ4 embryos at the 256-cell stage and high stage. Zebrafish Slbp2 can bind histone mRNA stem-loop in vitro, and the defects of MZΔ4 embryos can be partially rescued by overexpression of H2b. The current data indicate that maternal Slbp2 plays a pivotal role in the storage of replication-dependent histone mRNAs and proteins during zebrafish oogenesis.

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Section: Oocyte-specific and Maternal Expression Pattern Of Zebrafishsupporting

confidence: 53%

Section: Oocyte-specific and Maternal Expression Pattern Of Zebrafishmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis

Liu

et al. 2018

RNA

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“…And the full of length of CgDnd cDNA was further obtained by 3′ and 5′ RACE, using CgDnd F2, CgDnd R2 and the adaptor primer (5′-AAGCAGTGGTAACAACGCAGAGTAC-3′). The RACE-PCR was performed as preciously described (Liu et al, 2015b). The cDNA sequence has been submitted to GenBank with accession no.…”

Section: Full Length Cdna Cloning and Sequence Analysismentioning

confidence: 99%

Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)

Liu

et al. 2016

Gene

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Molecular characterization and expression patterns of stem-loop binding protein (SLBP) genes in protogynous hermaphroditic grouper, Epinephelus coioides

Liu

et al. 2019

Gene

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Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio

Cited by 4 publications

References 47 publications

Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis

Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis

Molecular characterization and expression pattern of a germ cell marker gene dnd in gibel carp (Carassius gibelio)

Molecular characterization and expression patterns of stem-loop binding protein (SLBP) genes in protogynous hermaphroditic grouper, Epinephelus coioides

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