Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp

Li, Xi-Yin; Li, Zhi; Zhang, Xiaojuan; Zhou, Li; Gui, Jian‐Fang

doi:10.1016/j.gene.2014.07.031

Cited by 32 publications

(21 citation statements)

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“…C. gibelio has become a promising model organism for understanding regulatory mechanisms of oocyte maturation, egg fertilization, and early embryogenesis (Gui and Zhou, 2010). Some important genes, such as cyclin A2 (Xie et al, 2001(Xie et al, , 2003, germ plasm markers vasa (Xu et al, 2005) and Dazl (Peng et al, 2009), C-type lectin (Dong et al, 2004), C1q-like (Mei et al, 2008a(Mei et al, ,b,2014, histone H2A variant h2af1o (Wu et al, 2009;Yue et al, 2013), maternal-effect factor spindlin (Sun et al, 2010), and testicular marker dmrt1 (Li et al, 2014a(Li et al, ,2014b, had been functionally characterized in C. gibelio (Gui and Zhu, 2012). Here, we identify an oocyte-specific slbp2 from C. gibelio, and characterize its expression and intracellular localization during oogenesis and early embryogenesis.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio

Liu

Zhang

Wang

et al. 2015

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio

Liu

Zhang

Wang

et al. 2015

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…After incubation 95°C for 5 min, the cycling protocol was followed by 39 cycles of 95°C for 5 s, 55°C for 15 s, 72°C for 30s. After amplification, a melting curve was performed according to the manufacturer recommendations to check the amplification specificity as described (Li et al 2014b). The specificity of amplification was also confirmed by agarose gel electrophoresis.…”

Section: Sequence Assembly and Annotationmentioning

confidence: 99%

A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish

Chen

Mei

et al. 2014

Mar Biotechnol

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Sex dimorphic growth pattern has significant theory and application implications in fish. Recently, a Y-and Xspecific allele marker-assisted sex control technique has been developed for mass production of all-male population in yellow catfish (Pelteobagrus fulvidraco), but the genetic information for sex determination and sex control breeding has remained unclear. Here, we attempted to provide the first insight into a comprehensive transcriptome covering multiple tissues from XX females, XY males, and YY super-males of yellow catfish by using 454 GS-FLX platform, for a better assembly and gene coverage. A total of 1,202,933 high quality reads (about 540 Mbp) were obtained and assembled into 28,297 contigs and 141,951 singletons. BLASTX searches against the NCBI non-redundant protein database (nr) led a total of 52,564 unique sequences including 18,748 contigs and 33,816 singletons to match 25,669 known or predicted unique proteins. All of them with annotated function were categorized by gene ontology (GO) analysis, and 712 were assigned to reproduction and reproductive process. Some potential genes relevant to reproductive system including steroid hormone biosynthesis and GnRH (gonadotropin-releasing hormone) signaling pathway were further identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis; and at least 21 sex determination and differentiation-related genes, such as Dmrt1, Sox9a/b, Cyp19b, WT1, and AMH were identified and characterized. Additionally, a total of 82,794 simple sequence repeats (SSRs), 26,450 single nucleotide polymorphisms (SNPs), and 4,145 insertions and deletions (INDELs) were revealed from the transcriptome data. Therefore, the current transcriptome resources highlight further studies on sex-control breeding in yellow catfish and will benefit future studies on reproduction and sex determination in teleost fish.

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“…The fertilized eggs of different developmental stages were fixed with 4% paraformaldehyde in PBS at 4°overnight. After washing with PBS three times, the nuclei were stained using DAPI, and the images were acquired under confocal microscopy as described Li et al 2014a). …”

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Extra Microchromosomes Play Male Determination Role in Polyploid Gibel Carp

Li¹,

Zhang²,

Zhang³

et al. 2016

Genetics

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Sex is generally determined by sex chromosomes in vertebrates, and sex chromosomes exhibit the most rapidly-evolving traits. Sex chromosome evolution has been revealed previously in numerous cases, but the association between sex chromosome origin and the reproduction mode transition from unisexual to sexual reproduction remains unclear. Here, we have isolated a male-specific sequence via analysis of amplified fragment length polymorphism from polyploid gibel carp (Carassius gibelio), a species that not only has the ability to reproduce unisexually but also contains males in wild populations. Subsequently, we have found through FISH analysis that males have several extra microchromosomes with repetitive sequences and transposable elements when compared to females. Moreover, we produced sex-reversed physiological females with a male-specific marker by using estradiol hormone treatment, and two gynogenetic families were established from them. In addition, the male incidence rates of two gynogenetic families were revealed to be closely associated with the extra microchromosome number of the sex-reversed physiological females. These results suggest that the extra microchromosomes in males might resemble a common feature of sex chromosomes and might play a significant role in male determination during the evolutionary trajectory of the reproduction mode transition from unisexual to sexual reproduction in the polyploid fish.KEYWORDS microchromosome; sex determination; sex chromosome; polyploid; gibel carp; genetics of sex S EX is a common phenomenon in nature and also one of the most important topics in life sciences, especially in evolutionary biology and genetics (Graves 2008). Most vertebrates are gonochoristic and reproduce by sexual reproduction. Different sex-determination systems, such as male heterogametic XX/XY sex chromosomes and female heterogametic ZZ/ZW sex chromosomes, as well as their numerous variants, have been revealed (Bachtrog et al. 2014;Mei and Gui 2015). Along with the rapid development of genomics and molecular genetic techniques, labile sex-determination systems and rapid sex chromosome turnovers have been noticed in both animals and plants recently (Bachtrog et al. 2014;Chen et al. 2014;Cortez et al. 2014;Graves 2014;Wei and Barbash 2015). Although how neo-sex chromosomes evolved (Roberts et al. 2009;Cortez et al. 2014;Vicoso and Bachtrog 2015) and how unisexual and sexual reproduction modes transformed (Jokela et al. 2009;Zhang et al. 2015) have been revealed, the association between sex chromosome origin and reproduction mode transition from unisexual to sexual reproduction remains unclear in vertebrates.The gibel carp, Carassius gibelio, has a wide geographic distribution in the Eurasian continent (Hanfling et al. 2005;Li and Gui 2008;Gui and Zhou 2010;Jakovlic and Gui 2011;Jiang et al. 2013) and diverse gynogenetic strains have been identified using biological traits and genetic markers (Zhou et al. 2000a;Yang and Gui 2004;Guo and Gui 2008). As a hexaploid with .150 chromosomes (...

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Expression characterization of testicular DMRT1 in both Sertoli cells and spermatogenic cells of polyploid gibel carp

Cited by 32 publications

References 59 publications

Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio

Molecular characterization and expression of an oocyte-specific histone stem-loop binding protein in Carassius gibelio

A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish

Extra Microchromosomes Play Male Determination Role in Polyploid Gibel Carp

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