Identification of 1-hydroxypyrene as a major metabolite of pyrene in pig urine

Keimig, Scott D.; Kirby, Kenneth W.; Morgan, Donald P.; Keiser, Jeffrey E.; Hubert, Terrance D.

doi:10.3109/00498258309052279

Cited by 63 publications

(19 citation statements)

References 5 publications

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“…Urinary 1-hydroxypyrene (1-OHP) has been widely used as a biological marker for evaluation of human exposure to polycyclic aromatic hydrocarbons (PAH) [1,2,3,4]. Pyrene is always present in PAH mixtures transported in the air particulate matter derived from car exhaust emission.…”

Section: Introductionmentioning

confidence: 99%

Development of a chemiluminescent enzyme immunoassay for urinary 1-hydroxypyrene

et al. 2002

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A chemiluminescent enzyme-immunoassay for urinary 1-hydroxypyrene has been developed and optimized. The enzymatic activity of horseradish peroxidase-labeled tracer was measured with an enhanced chemiluminescent system and the results were compared with those from conventional colorimetric detection. The method fulfilled all the requirements of accuracy and precision and the detection limit was 0.001 pmol/well, which enabled analysis in less than 1 microL urine. Subjects working in the center of Bologna who were exposed daily to vehicular exhaust gas were studied. Their urinary 1-hydroxypyrene concentrations were compared with the levels of benzo( a)pyrene in air particulate matter. Urinary 1-hydroxypyrene, which ranged from 0.5 to 10 nmol L(-1), correlated poorly with the concentration of benzo( a)pyrene in air particulate matter, which ranged from 5 to 140 ng m(-3). No significant effect of vehicle exhaust gas exposure was observed among the different groups of subjects working in different areas of the town. Thus, at a relatively low level of exposure 1-hydroxypyrene does not seem to be a sensitive biomarker of exposure to polycyclic aromatic hydrocarbons.

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Section: Introductionmentioning

confidence: 99%

Development of a chemiluminescent enzyme immunoassay for urinary 1-hydroxypyrene

et al. 2002

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“…Therefore, we tried to overcome this disadvantage by selecting out of infertility male patients only the men with normal semen parameters or with slight oligozoospermia according to WHO classification (1999) [29]. 1-hydroxypyrene (1-OHP) was first identified as a major metabolite of pyrene [42], accounting for about 90% of the total urinary excretion of pyrene basing on the data from the study using experimental animals. Urinary 1-OHP is considered an appropriate surrogate biomarker of total PAHs exposure of human populations [3,10,11] and it is increasingly used as a biomarker of human exposure to air pollution [15].…”

Section: Ijomeh 2013;26(5) 797mentioning

confidence: 99%

Association between a biomarker of exposure to polycyclic aromatic hydrocarbons and semen quality

Jurewicz¹,

Radwan²,

Sobala³

et al. 2013

International Journal of Occupational Medicine and Environmental Health

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Objectives: Growing evidence supports the reproductive and developmental toxicity of polycyclic aromatic hydrocarbons (PAHs) from prenatal and postnatal exposure, but the results of epidemiological studies regarding harmful effects of PAHs exposure on male reproductive system still remain limited and inconclusive. The aim of the present study was to investigate the relationship between 1-hydroxypyrene, a biomarker of polycyclic aromatic hydrocarbons exposure and semen quality. Materials and Methods:The study population consisted of 277 men attending an infertility clinic for diagnostic purposes and having normal semen concentration of 20-300 mln/ml or slight oligozoospermia (semen concentration: 15-20 mln/ml) (WHO 1999). All the men were healthy and under 45 years of age. All participants were interviewed and provided a semen sample. The interview included questions concerning demographics, socio-economic status, medical history related to past diseases which may have an impact on semen quality, lifestyle factors and occupational information. Concentrations of 1-hydroxypyrene (1-OHP) in the urine samples were analyzed using high performance liquid chromatography (HPLC). Results: A positive association was found between the level of 1-OHP in urine and sperm neck abnormalities as well as the percentage of static sperm cells (p = 0.001, p = 0.018, respectively). Additionally, exposure to PAHs measured by 1-OHP in urine decreased semen volume and the percentage of motile sperm cells (p = 0.014, p = 0.0001, respectively). Conclusions: Presented findings indicate that the environmental level of PAHs exposure adversely affects male semen quality. The future large-scale studies should incorporate different biomarkers to generate a more accurate and full assessment of the effects of PAHs exposure on male fertility.

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“…For urinary PAH biomarkers to be validated for wide application in human populations, a number of relevant factors should be examined: inter-and intraindividual variation, sensitivity and specificity of the measuring methods, and knowledge about the kinetics of occurrence and persistence of the biomarker (14 (17) and from coke oven workers (18) did not show differences in PAH levels in urine between exposed workers and nonexposed controls. Keimig et al (19) reported that 1-hydroxypyrene (1-OHP) is a major metabolite of pyrene in pig urine using high performance liquid chromatography (HPLC) with fluorescence detection and gas chromatography-mass spectrometry (GC-MS) confirmation. Jongeneelen et al (20)(21)(22)(23)(24) subsequently performed a series of studies evaluating the excretion of PAH metabolites in rodent and human urine.…”

Section: Introductionmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbon Metabolites in Urine as Biomarkers of Exposure and Effect

Strickland¹,

Kang²,

Sithisarankul³

1996

Environmental Health Perspectives

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Humans are exposed to polycyclic aromatic hydrocarbons (PAHs) from various occupational, environmental, medicinal, and dietary sources. PAH metabolites in human urine can be used as biomarkers of internal dose to assess recent exposure to PAHs. PAH metabolites that have been detected in human urine include 1-hydroxypyrene (1-OHP), 1-hydroxypyrene-O-glucuronide (1 -OHP-gluc), 3-hydroxybenzo[alpyrene, 7,8,9,1 0-tetrahydroxy-7,8 927-932 (1996)

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Identification of 1-hydroxypyrene as a major metabolite of pyrene in pig urine

Cited by 63 publications

References 5 publications

Development of a chemiluminescent enzyme immunoassay for urinary 1-hydroxypyrene

Development of a chemiluminescent enzyme immunoassay for urinary 1-hydroxypyrene

Association between a biomarker of exposure to polycyclic aromatic hydrocarbons and semen quality

Polycyclic Aromatic Hydrocarbon Metabolites in Urine as Biomarkers of Exposure and Effect

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