Identification, cloning and characterization of interleukin-17 and its family from zebrafish

Cloning and Characterization of Rainbow Trout Interleukin-17A/F2 (IL-17A/F2) and IL-17 Receptor A: Expression during Infection and Bioactivity of Recombinant IL-17A/F2

“…The expression of these molecules during infection and stimulation in vitro has been studied and the bioactivity of the IL-17A/F protein investigated for the first time in a nonmammalian species. The putative IL-17A/F protein consists of 146 aa and contains a predicted 25-aa signal peptide, suggesting that it is a secreted protein, as reported in other vertebrate species (7,16,17). Two copies of the mRNA instability motif ATTTA were present in the 3= untranslated region (UTR), as found in IL-17 molecules of other species, such as fugu (17), trout (62), and humans (7).…”

“…These mo- tifs are typical of proinflammatory molecules, which show transitory expression to reduce host damage (63), illustrating a potential role of the trout IL-17A/F in inflammatory responses. A multiple amino acid alignment revealed conservation of 4 cysteine residues for almost all the species considered, except for fugu IL-17A/F2 (17), which are signature residues in the IL-17 family (12,(16)(17)(18). The presence of 4 cysteine residues in trout and other IL-17A/F teleost molecules (16,17) suggests that they might be involved in the formation of 2 disulfide bonds, which form the cysteine knot characteristic of the IL-17 family (12,13).…”

“…A multiple amino acid alignment revealed conservation of 4 cysteine residues for almost all the species considered, except for fugu IL-17A/F2 (17), which are signature residues in the IL-17 family (12,(16)(17)(18). The presence of 4 cysteine residues in trout and other IL-17A/F teleost molecules (16,17) suggests that they might be involved in the formation of 2 disulfide bonds, which form the cysteine knot characteristic of the IL-17 family (12,13). Relative to glycosylation, 2 putative sites were found in trout, with potential glycosylation sites also reported in other IL-17A and IL-17F molecules (7,13,63).…”

Comparison of Two Chemically-Induced Colitis-Models in Adult Zebrafish, Using Optical Projection Tomography and Novel Transcriptional Markers

“…In zebrafish, three genes-IL-17A/F-1 to IL-17A/ F-3-have been identified which are considered to be homologous to IL-17A and IL-17F in higher vertebrates [57]. The expression of all three genes have been detected in both normal and LPS-stimulated intestinal tissue in zebrafish [57], and it is difficult to explain the inconsistent results obtained in this study with only IL-17A1 being significantly up-regulated (oxazolone group, 6 hpi). However, the different IL-17 forms may be associated with specific pathways.…”

Ciona intestinalis interleukin 17-like genes expression is upregulated by LPS challenge

“…These two genes are present in tandem on the same chromosome. Likewise, teleost IL-17 A and F homolog genes (IL17-A/F) are located on the same chromosome, and present the same genomic organization (three exons and two intron) (Gunimaladevi et al, 2006).…”