Cloning and Characterization of Rainbow Trout Interleukin-17A/F2 (IL-17A/F2) and IL-17 Receptor A: Expression during Infection and Bioactivity of Recombinant IL-17A/F2

Monte, Milena Mira; Wang, Tiehui; Holland, Jeremy; Zou, Jun

doi:10.1128/iai.00599-12

Cited by 84 publications

(78 citation statements)

References 82 publications

(155 reference statements)

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“…ChIL-17RA revealed a putative extracellular FnIII domain and an intracellular SEFIR domain, which are common structural characteristics of the IL-17R family (3,7,40). Multiple alignment of chIL-17RA between mammalian counterparts revealed a high degree of conservation within an intracellular SEFIR domain which shared a 68 to 72% identity.…”

Section: Discussionmentioning

confidence: 92%

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Downregulation of Chicken Interleukin-17 Receptor A during Eimeria Infection

et al. 2014

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e Both interleukin-17A (IL-17A) and IL-17F are proinflammatory cytokines that have an important role in intestinal homeostasis via receptor signaling. These cytokines have been characterized in chickens, but very little is known about their receptors and their functional activity. We provide here the first description of the sequence analysis, bioactivity, and comparative expression analysis of chicken IL-17RA (chIL-17RA) in chickens infected with Salmonella and Eimeria, two major infectious agents of gastrointestinal diseases of poultry of economic importance. A full-length chIL-17RA cDNA with a 2,568-bp coding region was identified from chicken thymus cDNA. chIL-17RA shares ca. 46% identity with mammalian homologues and 29.2 to 31.5% identity with its piscine counterparts. chIL-17RA transcript expression was relatively high in the thymus and in the chicken macrophage cell line HD11. The chIL-17RA-specific small interfering RNA inhibits interleukin-6 (IL-6), IL-8, and IL-1␤ mRNA expression in chicken embryo fibroblast cells (but not in DF-1 cells) stimulated with chIL-17A or chIL-17F. Interaction between chIL-17RA and chIL-17A was confirmed by coimmunoprecipitation. Downregulation of chIL-17RA occurred in concanavalin A-or lipopolysaccharide-activated splenic lymphocytes but not in poly(I·C)-activated splenic lymphocytes. In Salmonella-and Eimeria-infected chickens, the expression levels of the chIL-17RA transcript were downregulated in intestinal tissues from chickens infected with two Eimeria species, E. tenella or E. maxima, that preferentially infect the cecum and jejunum, respectively. However, chIL-17RA expression was generally unchanged in Salmonella infection. These results suggest that chIL-17RA has an important role in mucosal immunity to intestinal intracellular parasite infections such as Eimeria infection.

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Section: Discussionmentioning

confidence: 92%

“…mammalian IL-17RA) were also present in chIL-17RA. In rainbow trout IL-17RA, eight of the conserved cysteines were predicted to be involved in the formation of four disulfide bridges (40). The length of chIL-17RA amino acid sequence is shorter than vertebrate IL-17RA, except in the zebrafish (accession no.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of Chicken Interleukin-17 Receptor A during Eimeria Infection

et al. 2014

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“…They are expressed in the head, kidney and gill, and are upregulated by lipopolysaccharide stimulation. In the rainbow trout, Oncorhynchus mykiss, two IL-17C-like genes are modulated by LPS and IL-1β (Wang et al, 2010), and IL-17A/F2 molecule plays a role in promoting inflammatory and host innate immune responses directed against different pathogens (Monte et al, 2013). In addition, IL-17D homologs have been found in the Atlantic salmon, Salmo salar (Kumari et al, 2009), and in the lamprey, Lethenteron japonicum (Tsutsui et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Ciona intestinalis interleukin 17-like genes expression is upregulated by LPS challenge

Vizzini

Falco

Parrinello

et al. 2015

Developmental & Comparative Immunology

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“…Pro-inflammatory cytokines enable organisms to generate immunity in response to microbial invasion and inflammation [13]. Although primary investigations in rohu have contributed to extend our knowledge on role of various immune molecules during infections, responses related to cytokines remained unsolved in this species.…”

Section: Discussionmentioning

confidence: 99%

“…Polyinosinicpolycytidylic acid (poly I:C), a double stranded RNA virus analog mimics viral infection and in fish it has been reported to induce pro-inflammatory responses and anti-microbial activity [11]. It has been successfully used in vivo as well as in vitro to study expression pattern of cytokines in response to viral infection in several fish species [12][13][14][15]. In this study, we report in vitro expression analysis of pro-inflammatory cytokines IL-6, IL-8, IL-1β and TNFα in head-kidney leucocytes (important immune competent organ of fish) of rohu following stimulation with poly I:C and discuss its possible use in screening of immunostimulants or their use as inflammatory markers in viral immunity studies.…”

Section: Introductionmentioning

confidence: 99%

Expression profile of pro-inflammatory cytokines in head-kidney leucocytes of rohu following stimulation with poly I:C

Sahoo¹

2017

IJMBR

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IL (interleukin)-6, TNF (tumor necrosis factor)-α, IL-1β and IL-8 are pro-inflammatory cytokines which augment host inflammatory reactions in response to multiple stresses or infections. In this study, we analyzed expression profile of these cytokines in head kidney leucocytes (HKL) isolated from healthy rohu (Labeo rohita) juveniles in response to poly I:C (50 µg/ml), a synthetic dsRNA virus analog stimulation. RNA was isolated from stimulated HKLs at 0, 1, 3, 6, 12, 24, and 72 and 96 h post-stimulation (hps) and the expression profile of IL-6, TNF-α, IL-1β and IL-8 was studied using qPCR. IL-6 expression was found to be significantly up-regulated at 72 hps whereas higher level of TNF-α was evident at 96 hps. With regard to IL-8 and IL-1β, a similar kind of expression pattern was observed where levels of both cytokines were higher at 24 hps. As activation of pro-inflammatory cytokine cascade against infection is considered as a marker of early immunity, induction of cytokine levels at or after 24 hps in rohu HKLs following poly I:C stimulation suggested the potential inflammatory role played by these cytokines during viral infection in fish.

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Cloning and Characterization of Rainbow Trout Interleukin-17A/F2 (IL-17A/F2) and IL-17 Receptor A: Expression during Infection and Bioactivity of Recombinant IL-17A/F2

Cited by 84 publications

References 82 publications

Downregulation of Chicken Interleukin-17 Receptor A during Eimeria Infection

Downregulation of Chicken Interleukin-17 Receptor A during Eimeria Infection

Ciona intestinalis interleukin 17-like genes expression is upregulated by LPS challenge

Expression profile of pro-inflammatory cytokines in head-kidney leucocytes of rohu following stimulation with poly I:C

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