Identification, cellular localization, isolation, and characterization of human Clara cell-specific 10 KD protein.

Singh, Gurmukh; Singh, Jagjit; Katyal, Sikandar L.; Brown, William E.; Kramps, J. A.; Paradis, Irwin L.; Dauber, James H.; Macpherson, Trevor A.; Squeglia, Nicholas

doi:10.1177/36.1.3275712

Cited by 176 publications

(118 citation statements)

References 17 publications

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“…It would be reasonable that the distribution of these two polypeptides in cancer tissues compared with that of known polypeptides would resemble that of surfactant apoprotein A, Clara cell-specific 10-kDa protein and protein 1 in primary lung carcinoma (Singh et al, 1988;Auten et al, 1990; Barnard et al, 1992;Linnoila et al, 1992). The molecular weights of Clara cellspecific 10-kDa protein and protein 1 are completely different from those of TAO1 and TA02 polypeptides.…”

Section: Discussionmentioning

confidence: 99%

Relationship between TA01 and TA02 polypeptides associated with lung adenocarcinoma and histocytological features

Hirano¹,

Fujioka²,

Franzén³

et al. 1997

Br J Cancer

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Summary TA01 (molecular weight 35.0 kDa, isoelectric point 5.45) and TA02 (molecular weight 35.0 kDa, isoelectric point 5.29) polypeptides were detected using two-dimensional polyacrylamide gel electrophoresis (2-DE). A previous study has shown that these polypeptides are distributed in primary adenocarcinomas and some large -cell carcinomas of the lung. However, various expression levels of TA01 and TA02 polypeptides were demonstrated in adenocarcinoma, while large-cell carcinoma expressed low levels. To evaluate the relationship between the expression of TA01 and TA02 polypeptides and the histocytological features of primary adenocarcinoma of the lung, these two polypeptides were analysed by 2-DE combined with a non-enzymatic sample preparation technique, and their expression levels were compared with the histocytological features of primary lung adenocarcinoma. Out of 57 primary lung adenocarcinoma cases, 46 cases (80.7%) and 52 cases (91.2%) expressed TA01 and TA02 polypeptides respectively. Furthermore, the expression levels of TA01 and TA02 polypeptides correlated with the degree of cellular atypia, structural atypia and histocytological differentiation of primary lung adenocarcinoma. On the other hand, these two polypeptides were not detected in adenocarcinoma of the lung, metastatic from the colon and mammary glands. High expression of TA01 and TA02 polypeptides reflected the differentiation of primary adenocarcinoma in the lung. These two polypeptides are valuable in determining the histocytological differentiation of primary lung adenocarcinoma as well as in distinguishing between primary and metastatic adenocarcinoma of the lung.Keywords: primary adenocarcinoma of the lung; histopathological differentiation; TA01 polypeptide; TA02 polypeptide; two-dimensional polyacrylamide gel electrophoresis Tumour markers can contribute to clinicopathological diagnosis, more accurate evaluation of biological malignancy of the tumour and selection of therapeutic strategy. Products of oncogenes and mutant tumour-suppressor genes are possible candidates for biological tumour markers. Also, histological differentiation antigens could be useful markers in cases of borderline histology.Although some tumour markers (e.g. CEA, CA 19-9 and TPA, etc.) are used in the clinical diagnosis of primary adenocarcinoma of the lung (Vincent et al, 1979;Latanche et al, 1985;Buccheri et al, 1988;Niklinski et al, 1995), there is no specific tumour marker for primary adenocarcinoma of the lung. At present, cell proliferation and products of oncogenes and tumour-suppressor genes in lung adenocarcinoma are attracting attention (M0rkve et al, 1992;Scagliotti et al, 1993;Ebina et al, 1994;Rachwal et al, 1995;Costa et al, 1996) Correspondence to: T Hirano Linnoila et al, 1992) and protein 1 (Barnard et al, 1992). There is a possibility that these polypeptides become specific tumour markers for primary adenocarcinoma of the lung. Such a marker might be useful to distinguish primary and metastatic adenocarcinoma of the lung.We also investigate...

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Section: Discussionmentioning

confidence: 99%

Relationship between TA01 and TA02 polypeptides associated with lung adenocarcinoma and histocytological features

Hirano¹,

Fujioka²,

Franzén³

et al. 1997

Br J Cancer

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“…Currently, this protein is known by several names, which are primarily derived from the organ or body fluid in which it is detectable or from the type of xenobiotics with which it interacts. Thus, it is called the progesterone-binding protein [8], Clara cell 10 kDa protein [9], urine protein-1 [7], polychlorinated biphenyl-binding protein [10], and retinol-binding protein [11]. Recently, the UG/Clara cell family of proteins has been classified as members of a new superfamily called ''secretoglobins'' [12].…”

mentioning

confidence: 99%

Lys 43 and Asp 46 in α-helix 3 of uteroglobin are essential for its phospholipase A2 inhibitory activity

Chowdhury¹,

Mantile-Selvaggi²,

Miele³

et al. 2002

Biochemical and Biophysical Research Communications

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Uteroglobin (UG) is an anti-inflammatory, secreted protein with soluble phospholipase A 2 (sPLA 2 )-inhibitory activity. However, the mechanism by which UG inhibits sPLA 2 activity is unknown. UG is a homodimer in which each of the 70-amino acid subunits forms four a-helices. We previously reported that sPLA 2 -inhibitory activity of UG may reside in a segment of a-helix 3 that is exposed to the solvent. In addition, it has been suggested that UG may inhibit sPLA 2 activity by binding and sequestering Ca þþ , essential for sPLA 2 activation. By site-specific mutation, we demonstrate here that Lys 43 Glu, Asp 46 Lys or a combination of the two mutations in the full-length, recombinant human UG (rhUG) abrogates its sPLA 2 -inhibitory activity. We demonstrate further that recombinant UG does not bind Ca þþ although when it is expressed with histidine-tag (H-tag) it is capable of binding Ca þþ . Taken together our results show that: (i) Lys 43 and Asp 46 in rhUG are critical residues for the sPLA 2 -inhibitory activity of UG and (ii) Ca þþ -sequestration by rhUG is not likely to be one of the mechanisms responsible for its sPLA 2 -inhibitory activity. Ó 2002 Elsevier Science (USA). All rights reserved.

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“…In human lung, after birth, TTF-1 is detected in nuclei of subsets of epithelial cells, both in the conducting airway and in type II epithelial cells (25,26). In the lung, TTF-1 expression overlaps with that of SP-A, SP-B, SP-C, and CCSP, but its distribution is more extensive than that of the surfactant proteins or CCSP (27)(28)(29). Although TTF-1 strongly influences the transcription of each of these genes, each has a distinct temporal and spatial pattern of expression, supporting the concept the activity of TTF-1 may be further modified to confer the distinct regulatory features typical of each gene.…”

Section: Discussionmentioning

confidence: 99%

Protein Kinase A Activation of the Surfactant Protein B Gene Is Mediated by Phosphorylation of Thyroid Transcription Factor 1

Yan

Whitsett

1997

Journal of Biological Chemistry

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Identification, cellular localization, isolation, and characterization of human Clara cell-specific 10 KD protein.

Cited by 176 publications

References 17 publications

Relationship between TA01 and TA02 polypeptides associated with lung adenocarcinoma and histocytological features

Relationship between TA01 and TA02 polypeptides associated with lung adenocarcinoma and histocytological features

Lys 43 and Asp 46 in α-helix 3 of uteroglobin are essential for its phospholipase A2 inhibitory activity

Protein Kinase A Activation of the Surfactant Protein B Gene Is Mediated by Phosphorylation of Thyroid Transcription Factor 1

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