Identification and quantification of triterpenoid centelloids in<i>Centella asiatica</i>(L.) Urban by densitometric TLC

James, Jacinda T.; Dubery, Ian A.

doi:10.1556/jpc.24.2011.1.16

Cited by 58 publications

(40 citation statements)

References 16 publications

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“…Other triterpenoids in Gotu kola include: asiaticoside C, D, E, F; centellasaponin B, C; isothankunic acid and oleanane type saponins, e.g. terminolic acid; centellasaponin D. C. asiatica contains about 0.1% essential oils with α‐humulene, germacrene B/D, β‐caryphyllene, flavonoids, sesquiterpenes, steroids (Brinkhaus et al ., ; James and Duebery, ; James and Dubery, ; Nhiem et al ., ). Saponins may account for 1% to 8%, according to the European Pharmacopoeia , not less than 6.0% (Ph.Eur.…”

Section: Chemical Constituentsmentioning

confidence: 99%

Centella asiaticain Dermatology: An Overview

et al. 2014

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Centella asiatica is a medicinal plant that was already used as a 'panacea' 3000 years ago. The active compounds include pentacyclic triterpenes, mainly asiaticoside, madecasosside, asiatic acid and madecassic acid. We have conducted an overview to summarize current knowledge on the results of scientific in vitro and in vivo experiments focused on the improvement of the healing process of small wounds, hypertrophic scars and burns by C. asiatica. In this paper, we discuss the data on constituents, recommended preparations and the potential side effects of C. asiatica.

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Section: Chemical Constituentsmentioning

confidence: 99%

Centella asiaticain Dermatology: An Overview

et al. 2014

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“…The triterpenoids represent one of the important class of secondary metabolites, which are difficult to separate on high‐performance liquid chromatography (HPLC) due to similar structure and physico‐chemical properties 14, 15. Although quantification of triterpenoids by high‐performance thin‐layer chromatography (HPTLC) is a difficult proposition due to the lack of chromophores and low UV sensitivity, the use of this methodology has become practical by resorting to post chromatographic derivatization 16–18. HPTLC is a globally accepted rational and practical solution to characterize the crude plant drug, pharmacologically active constituents, enriched standardized extracts and their formulations.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of five marker compounds of Betula utilis stem bark using a validated high‐performance thin‐layer chromatography method

Khan

Sangwan

Dhar

et al. 2011

J of Separation Science

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A sensitive, selective and robust densitometric high-performance thin-layer chromatographic method was developed and validated for five marker compounds, namely betulin, lupeol, oleanolic acid, 3-acetyloleanolic acid and β-sitosterol, known for their various therapeutic activities. The marker compounds have been isolated from the stem bark of Betula utilis, well characterized by the spectral analysis, and their simultaneous quantitative determination carried out by high-performance thin-layer chromatography (HPTLC) method. The resolution of marker compounds was carried out on silica-gel 60 plates, using n-hexane:ethyl acetate (8:2 v/v) as the mobile phase. The HPTLC densitometry was performed at 500-nm wavelength after the post chromatographic derivatization with ceric ammonium sulfate reagent. The optimized method provided good linear relation (r>0.9960) for all the investigated analytes. The method is simple, and reproducible, which may be applied for quantitative analysis of the above-mentioned marker compounds.

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“…4 Asiaticoside has a molecular weight of 959.12. 12 Active substances with small molecular weight can penetrate faster as compared to the active substances with a high molecular weight. 24 In addition, the physicochemical factors that can affect the penetration is the partition coefficient of the drug in the n-octanol-water.…”

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confidence: 99%