Identification and quantification of hemoglobins A, F, S, and C by automated chromatography

Papadea, Christine; Cate, John C.

doi:10.1093/clinchem/42.1.57

Cited by 42 publications

(12 citation statements)

References 6 publications

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“…Recently, there has been increased interest in applying proteomics to clinical diagnostics. Generally, isoelectrofocusing (IEF) and cation exchange HPLC have been used to detect variants of human hemoglobin [33,34]. RP-HPLC separation of a and b chains can also provide some useful information [14].…”

Section: Separation Of a And B Chains Of Human Hemoglobinmentioning

confidence: 99%

Fabrication of a poly(styrene–octadecene–divinylbenzene) monolithic column and its comparison with a poly(styrene–divinylbenzene) monolithic column for the separation of proteins

Chen

et al. 2007

J of Separation Science

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In this paper, a poly(styrene-octadecene-divinylbenzene) (PS-OD-DVB) monolithic column was prepared in one step by introducing a C18 carbon chain as monomer. N,N-Dimethylformamide and decanol served as porogens to make a homogeneous polymerization mixture in a fused silica capillary (320 microm inner diameter). Its physical and chromatographic properties were compared with those of poly(styrene-divinylbenzene) (PS-DVB) monolithic column, which was also fabricated by in-situ polymerization in a fused silica capillary with the same inner diameter. Six standard proteins were used to evaluate the columns and their potential application for the separation of human hemoglobin was also discussed. It was shown that the PS-OD-DVB and PS-DVB monoliths appeared to have similar efficiency for rapid separation of six proteins within 3.5 min. The PS-OD-DVB monolith was found to have higher loading capacity and higher resolution for the separation of alpha and beta chains of hemoglobin because of the introduction of C18 carbon chains, and shows great potential for the separation of bio-macromolecules.

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Section: Separation Of a And B Chains Of Human Hemoglobinmentioning

confidence: 99%

Fabrication of a poly(styrene–octadecene–divinylbenzene) monolithic column and its comparison with a poly(styrene–divinylbenzene) monolithic column for the separation of proteins

Chen

et al. 2007

J of Separation Science

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“…Pre-and post-pheresis complete blood counts (CBC) and Hb S levels are also obtained. Hb S is quantitated by highperformance liquid chromatography (HPLC) [12].…”

Section: Erythrocytapheresis Protocolmentioning

confidence: 99%

Erythrocytapheresis limits iron accumulation in chronically transfused sickle cell patients

et al. 1998

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Cerebrovascular accidents (CVA) as a complication of sickle cell disease occur most frequently in childhood. Life-long transfusion prevents recurrent stroke, but inevitably leads to iron overload. Although effective chelation exists, many patients are not compliant. Erythrocytapheresis, an automated method of red blood cell exchange, was evaluated as an alternative to control transfusion-related iron load. Eleven patients with sickle cell anemia and a history of stroke were converted from simple transfusion to pheresis. Total time on pheresis for the group averaged 19 months (range 4-36 months). No significant complications occurred with a mean pre-pheresis hemoglobin S (Hb S) level of 44%. Blood utilization increased by an average of 50%. The effect of pheresis on serum ferritin depended on the patient's pre-pheresis ferritin level and chelation regimen. Ferritin levels remained stable for chelated patients with ferritin levels > or = 5,000 ng/ml, but decreased in a chelated patient with a pre-pheresis ferritin level of 4,000 ng/ml. For non-chelated patients with significant pre-pheresis iron load, ferritin levels remained stable. No patient on chelation prior to pheresis was able to discontinue deferoxamine. However, one patient with pre-pheresis ferritin of 500 ng/ml maintained serum ferritin levels < 200 ng/ml for 36 months of pheresis without chelation. Pheresis is more expensive than simple transfusion unless the cost of chelation and organ damage from iron overload are considered. Erythrocytapheresis is a safe method of controlling Hb S levels and limiting or preventing iron load in chronically transfused sickle cell patients.

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“…Quantitation of HbA2, HbF and HbS Table 1 Mean HbF level in sickle-cell anaemia patients from Brazzaville. Congo was obtained by cation-exchange high-performance liquid chromatography (HPLC) (9). DNA was extracted from leukocytes by standard phenolchloroform extraction and ethanol precipitation.…”

Section: Letters To the Editormentioning

confidence: 99%

Haemoglobin F (HbF) levels in sickle‐cell anaemia patients homozygous for the Bantu haplotype

Mouélé

Galactéros

Feingold

1999

European J of Haematology

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Identification and quantification of hemoglobins A, F, S, and C by automated chromatography

Cited by 42 publications

References 6 publications

Fabrication of a poly(styrene–octadecene–divinylbenzene) monolithic column and its comparison with a poly(styrene–divinylbenzene) monolithic column for the separation of proteins

Fabrication of a poly(styrene–octadecene–divinylbenzene) monolithic column and its comparison with a poly(styrene–divinylbenzene) monolithic column for the separation of proteins

Erythrocytapheresis limits iron accumulation in chronically transfused sickle cell patients

Haemoglobin F (HbF) levels in sickle‐cell anaemia patients homozygous for the Bantu haplotype

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