Fabrication of a poly(styrene–octadecene–divinylbenzene) monolithic column and its comparison with a poly(styrene–divinylbenzene) monolithic column for the separation of proteins

Abstract

In this paper, a poly(styrene-octadecene-divinylbenzene) (PS-OD-DVB) monolithic column was prepared in one step by introducing a C18 carbon chain as monomer. N,N-Dimethylformamide and decanol served as porogens to make a homogeneous polymerization mixture in a fused silica capillary (320 microm inner diameter). Its physical and chromatographic properties were compared with those of poly(styrene-divinylbenzene) (PS-DVB) monolithic column, which was also fabricated by in-situ polymerization in a fused silica capillary with the same inner diameter. Six standard proteins were used to evaluate the columns and their potential application for the separation of human hemoglobin was also discussed. It was shown that the PS-OD-DVB and PS-DVB monoliths appeared to have similar efficiency for rapid separation of six proteins within 3.5 min. The PS-OD-DVB monolith was found to have higher loading capacity and higher resolution for the separation of alpha and beta chains of hemoglobin because of the introduction of C18 carbon chains, and shows great potential for the separation of bio-macromolecules.

Poly(divinylbenzene-alkyl methacrylate) monolithic stationary phases in capillary electrochromatography

“…The result of surface area measurement also indicated that longer alkyl methacrylate monomer produced lower surface area of stationary phase (Table 1), and this would be another reason for the faster benzophenone separation in the poly(DVB-LMA). Previous reports have demonstrated that a reduction in the amount of benzene in polystyrene-based materials could improve peak-tailing problem [18,[26][27][28], and this was similar to the results of poly(S-DVB-BMA) and poly(DVB-BMA) reported in Section 3.1. In this case, by using different carbon length of alkyl methacrylate monomer to prepare the polystyrene-based monoliths, it changed the strength of -interaction, and it in turn enhanced the peak symmetry for aromatic compound separation on polystyrenebased stationary phase.…”

“…Henion et al and Legido-Quigley et al reported surface-alkylated poly(S-DVB) monoliths prepared by postpolymerization alkylation and the surface octadecylation process provided better separation and lower limits of detection for peptide and protein analyses [26,27]. Fang et al also reported alkylated poly(S-DVB) monoliths that were prepared by a single step, and sample loading capacity and resolution of several biomolecules were improved by the addition of a C18 long carbon chain on the polymeric monoliths [28]. These reports demonstrated the peak-tailing problem was successfully reduced by decreasing the benzene moieties on polystyrene-based stationary phase.…”

Surfactant-bound monolithic columns for separation of proteins in capillary high performance liquid chromatography

“…The procedure can be found elsewhere [41,42]. Subsequently, AAUA, EDMA, 1-propanol, 1,4-butanediol, water, and AIBN were mixed ultrasonically into a homogenous solution and purged with nitrogen for 10 min.…”

Analysis of microcystins by capillary high performance liquid chromatography using a polymethacrylate‐based monolithic column

“…www.jss-journal.com [35]. In brief, the capillary was first rinsed with acetone for 10 min, and then activated with 0.1 mol/L sodium hydroxide at 1008C for 2 h. After washing with water and 0.1 mol/L hydrochloric acid for 30 min, the capillary was washed again with water and then with acetone, and subsequently dried with nitrogen gas.…”