Identification and Profile of Furanocoumarins from the Ribbed Celery (<i>Apium Graveolens</i> L Var. <i>Dulce</i> Mill./ Pers.)

Abstract

The aim of this study was to identify and determine the qualitative and quantitative composition of furanocoumarins in anatomical parts (blade and petiole) of two cultivars of ribbed celery, depending on the age of the plant . Two accurate, precise and inexpensive techniques for analytical detection of coumarin in a relatively short period of time were used in the present study. TLC -the method considered as preliminary, showed the presence of psolaren, bergapten, xanthotoxin and simple coumarin -umbelliferone . Additionally, isopimpinellin was detected by HPLC method . Chromatographic analysis of ribbed celery leaves showed the presence of four furanocoumarins . Psoralen and isopimpinellin are dominant in the leaf blades, wherein content is correlated with the term of harvesting . Bergapten was dominant in petioles regardless of the harvesting time and cultivar.Keywords: Apiaceae, cultivars, harvest, furanocoumarin, HPLC methods IntroductionLeafy vegetables are an important group of plants in the daily diet . Less known species like endive, chicory, arugula, rochet and chard has become popular among consumers . Also ribbed celery (Apium graveolens L . var . Dulce Mill . / Pers . ), valued for its attractive appearance, taste and the nutritional composition resulting from the rich mineral and vitamin composition is classified to this group (Vogel, 1996) . In part for consumption (petioles and partially leaf blades) contains many valuable biologically active substances such as phenolic acids, flavonoids, tannins, mono-and sesquiterpenes which make them useful in the treatment of certain dermatological diseases, arthritic-rheumatic, urinary and digestive system (Williamson, 1999;Wolski et al . , 2002) .Plants from the family Apiaceae contain compounds with multidirectional biological activity: antimicrobial, anti-cancer, for the liver agents and cyclooxygenase inhibitors (Chen et al . , 1995;Strohl, 2000) . The presence of these substances is responsible for the sealing properties and capillary walls strengthens, improving blood circulation in the myocardium, spasmolytic, diuretic, platelet anticoagulation, anti-ulcer, anti-allergic, anti-inflammatory, antihepatotoxic, antifungal and antiviral effects Quiros, 1988, Zheng et al . , 1993) .Coumarins are fairly common in many fruits and vegetables, especially in the families Apiaceae and Rutaceae (Zobel and Brown, 1990;Ojala, 2001) . These group of compounds are important from A. Najda et al. 68 the therapeutic point of view (Leal et al . , 2000; WaksmundzkaHajnos et al . , 2004) . Currenly, furanocoumarins of natural origin are used in PUVA therapy of vitiligo and psoriasis; furthermore, they are active Ca +2 channel blockers (Murray et al . , 1982;Vuorela et al . , 1988;Coven et al . , 1999) . Furanocoumarins are characterized by a broad spectrum of biological activity . They reduce the platelet agregation in in vitro test (Chen et al . ,1996), they induce apoptosis in human promyelocytic leukemia, HL-60 cells (Bogucka-Kocka and Kocki, 2002;Pae et al . , 2...

The celery genome sequence reveals sequential paleo‐polyploidizations, karyotype evolution and resistance gene reduction in apiales

“…Celery seed‐derived oil has been extracted to repel lesser grain borer, dengue vector, mosquito and bacteria (Baananou et al ., 2013; Ebadollahi, 2018; Kumar et al ., 2014; Tuetun et al ., 2004). In addition, celery and other Apiaceae species are rich in coumarins and their derivatives (Najda et al ., 2015; Numonov et al ., 2018; Poumale et al ., 2013; Zobel and Brown, 1990), a major group of plant secondary metabolites with multiple pharmaceutical activities and important roles in defence against pathogens (Carpinella et al ., 2005; Chong et al ., 2002; Figueroa‐Guinez et al ., 2015; Kontogiorgis et al ., 2006; Stanchev et al ., 2008; Sun et al ., 2015). Here we showed that several genes of the coumarin biosynthesis pathway in celery and some other Apiaceae species were expanded in copy number compared with Arabidopsis and other studied non‐Apiaceae species.…”

“…In addition to being an important vegetable, celery has many pharmacologically active compounds, including flavonoids, volatile oils, unsaturated fatty acids and others (Lin et al ., 2007; Sellami et al ., 2012). It is rich in coumarins and their derivatives (Najda et al ., 2015; Numonov et al ., 2018; Poumale et al ., 2013; Zobel and Brown, 1990), having prominent roles in defending against pathogens (Carpinella et al ., 2005; Chong et al ., 2002; Figueroa‐Guinez et al ., 2015; Sun et al ., 2015) and multiple pharmaceutical activities, such as anticoagulation, antibacterial and anti‐inflammatory properties (Kontogiorgis et al ., 2006; Stanchev et al ., 2008).…”

Dietary Intake of Coumarins and Furocoumarins through Citrus Beverages: A Detailed Estimation by a HPLC-MS/MS Method Combined with the Linear Retention Index System

“…Furocoumarins inhibit the calcium channel, reduce platelet aggregation [ 9 ], induce apoptosis in human promyelocytic leukaemia [ 10 ] and show hepatoprotective effects [ 11 ]. Imperatorin has been reported to have numerous potent pharmacological actions, including anti-inflammation [ 12 ], anti-bacterial [ 13 ], anti-allergic [ 14 ], beneficial cardiovascular effects [ 15 , 16 , 17 ], neuromodulation [ 18 , 19 , 20 ] and is able to induce apoptosis in human promyelocytic leukaemia and HL-60 cells [ 10 ].…”

Phytochemical analysis and anticancer activity of Falcaria vulgaris Bernh growing in Moghan plain, northwest of Iran

“…Various studies have shown that plant compounds play an important role in both the prevention and treatment of cancers. These compounds work by different mechanisms; however, the induction of apoptosis is a common point of many of these compounds [ 38 ]. The effect of coumarins on the cytotoxic effect of members of the Apicaceae family has also been proven in studies [ 39 – 41 ].…”