The aim of this study was to identify and determine the qualitative and quantitative composition of furanocoumarins in anatomical parts (blade and petiole) of two cultivars of ribbed celery, depending on the age of the plant . Two accurate, precise and inexpensive techniques for analytical detection of coumarin in a relatively short period of time were used in the present study. TLC -the method considered as preliminary, showed the presence of psolaren, bergapten, xanthotoxin and simple coumarin -umbelliferone . Additionally, isopimpinellin was detected by HPLC method . Chromatographic analysis of ribbed celery leaves showed the presence of four furanocoumarins . Psoralen and isopimpinellin are dominant in the leaf blades, wherein content is correlated with the term of harvesting . Bergapten was dominant in petioles regardless of the harvesting time and cultivar.Keywords: Apiaceae, cultivars, harvest, furanocoumarin, HPLC methods IntroductionLeafy vegetables are an important group of plants in the daily diet . Less known species like endive, chicory, arugula, rochet and chard has become popular among consumers . Also ribbed celery (Apium graveolens L . var . Dulce Mill . / Pers . ), valued for its attractive appearance, taste and the nutritional composition resulting from the rich mineral and vitamin composition is classified to this group (Vogel, 1996) . In part for consumption (petioles and partially leaf blades) contains many valuable biologically active substances such as phenolic acids, flavonoids, tannins, mono-and sesquiterpenes which make them useful in the treatment of certain dermatological diseases, arthritic-rheumatic, urinary and digestive system (Williamson, 1999;Wolski et al . , 2002) .Plants from the family Apiaceae contain compounds with multidirectional biological activity: antimicrobial, anti-cancer, for the liver agents and cyclooxygenase inhibitors (Chen et al . , 1995;Strohl, 2000) . The presence of these substances is responsible for the sealing properties and capillary walls strengthens, improving blood circulation in the myocardium, spasmolytic, diuretic, platelet anticoagulation, anti-ulcer, anti-allergic, anti-inflammatory, antihepatotoxic, antifungal and antiviral effects Quiros, 1988, Zheng et al . , 1993) .Coumarins are fairly common in many fruits and vegetables, especially in the families Apiaceae and Rutaceae (Zobel and Brown, 1990;Ojala, 2001) . These group of compounds are important from A. Najda et al. 68 the therapeutic point of view (Leal et al . , 2000; WaksmundzkaHajnos et al . , 2004) . Currenly, furanocoumarins of natural origin are used in PUVA therapy of vitiligo and psoriasis; furthermore, they are active Ca +2 channel blockers (Murray et al . , 1982;Vuorela et al . , 1988;Coven et al . , 1999) . Furanocoumarins are characterized by a broad spectrum of biological activity . They reduce the platelet agregation in in vitro test (Chen et al . ,1996), they induce apoptosis in human promyelocytic leukemia, HL-60 cells (Bogucka-Kocka and Kocki, 2002;Pae et al . , 2...
Stevia rebaudiana is a natural sweetener herb that is increasingly used in herbal medicines in the food and cosmetics industries. Molecular methods can be combined with morphological techniques to identify stevia genotypes as a starting material to produce more reliable bioproducts. This study evaluated the level of the genetic and biochemical diversity in various stevia genotypes using HPLC (high performance liquid chromatography) analysis and random amplified polymorphic DNA (RAPD) markers. Stevia genotypes collected from different locations of the world showed clear variations at the biochemical and genetic level in Polish climate conditions. The influence of the genotypes on the content of steviol glycosides, antioxidants, phenols, flavonoids, and tannins was analyzed using phytochemical assays. Genotypes from Morocco, Poland, Egypt, and Nigeria can be defined as samples of higher quality compared to other genotypes analyzed in terms of the amount of steviol glycosides. Considering the rebaudioside A/stevioside ratio as a selection criterion, genotypes from Australia, China, India, and Pakistan should be considered to be valuable in terms of suitability for obtaining new varieties. The present results of RAPD marker analysis indicated differential banding pattern and considerable polymorphism among all ten stevia genotypes. Genotypes from Morocco, Egypt, Poland, Nigeria, China, and India, as genetically different, can be selected for further stevia breeding programs.
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