“…Thin layer chromatography (TLC) finds its place when the relatively costly equipment required by other methods is unavailable. Thin layer chromatography is an important tool for the identification of amino acids by various spray reagents (Lorenz and Flatter, 1970;Devaux and Mesnard, 1971;Wolski et al, 1980;Distler and Fresenius, 1981;Laskar and Basak, 1988;1990;Basak and Laskar, 1990;Laskar et al 1991;2001a,b,c;Basak et al, 1993;Sinhababu et al, 1994;2013;Khawas and Laskar 2003a,b;Khawas et al, 2004;Samanta et al, 2004;Samanta and Laskar, 2006;Das et al, 2010;Sahana et al, 2011). Of the reagents in general use, ninhydrin is the most popular because of its remarkably high sensitivity (Stahl, 1969).…”