Modified ninhydrin reagents to detect amino acids on TLC plates

Sinhababu, Amalendu; Basu, S.; Dey, Haradhan

doi:10.1007/s11164-013-1388-5

Cited by 13 publications

(3 citation statements)

References 14 publications

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“…The solvent system used for the separation of amino acids consists of n -butanol, water, and acetic acid in a ratio of 60 : 25 : 15. Ninhydrin spray was employed to detect amino acids on the TLC plate [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Dar

Ahmad

Samin

et al. 2023

International Journal of Analytical Chemistry

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A novel pressurized flow system for circular thin-layer chromatography (PC-TLC) has been successfully established and employed for the separation of amino acids, dyes, and pigments for safe medical imaging applications. In this system, the mobile phase is applied to a regular TLC plate through the tube and needle of an intravenous infusion set. The needle was fused in a hole underneath the center of the plate, while the second side end of the tube was connected to a microburette containing the solvent. This new assembly proved itself better in terms of separation time (within 5 minutes) and controlled flow of the solvent and horizontal movement of analyte components over chromatograms with better separation and Rf values (glutamine: 0.26, valine: 0.44, phenylalanine: 0.60, chlorophyll a: 0.52, chlorophyll b: 0.43, xanthophyll: 0.18, carotenoid: 0.97, and pheophytin: 0.60) when a number of samples of amino acids, dyes, and pigments were separated by the developed apparatus and the conventional TLC procedure. The developed method was found distinctly rapid, precise, and eco-friendly (less solvent consuming) as compared to traditional ascending TLC.

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Section: Methodsmentioning

confidence: 99%

Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Dar

Ahmad

Samin

et al. 2023

International Journal of Analytical Chemistry

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“…Moreover, the visualization of TLC plate with several reagents aimed to detect the presence of particular bioactive groups thoroughly. For instance, the Dragendorff reagent was used for detecting alkaloids, vanillin in H 2 SO 4 for terpenes/ steroids derivatives, FeCl 3 for phenol, ninhydrin for free amino acids, and DPPH for antioxidant compounds (de Moura et al, 2016;Singh Rana & Saklani, 2017;Sinhababu et al, 2015;.…”

Section: Metabolites Characterizationmentioning

confidence: 99%

Metabolites Alteration and Antioxidant Activity of Gracilaria verrucosa After Fermentation Using Aureobasidium melanogenum MTGK.31

Sibero

Savitri

Frederick³

et al. 2023

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Gracilaria verrucosa is a red seaweed that has been widely utilized in the food andpharmaceutical industries due to its biological properties. The utilization of biologicalagents in obtaining certain bioactive compounds would confront unavoidable issues,particularly its bioactive sustainability. Hence, microbial fermentation has been reported as a practical approach to maintaining bioactive production and boosting its properties. Our study aimed to evaluate the potential of marine yeast Aureobasidium melanogenum MTGK.31 as a fermenting agent for G. verrucosa and characterize the seaweed metabolite profile and antioxidant activity after fermentation. The seaweed was fermented using A. melanogenum MTGK.31 in a medium consisting of yeast extract, peptone, and glucose. The fermentation was done for 24, 48, and 72 hours. Total plate count and pH were measured after each fermentation period. The primary and secondary metabolites of G. verrucosa in each fermentation were observed. Antioxidant assay using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method was conducted, followed by total phenolic content using the Folin-Ciocalteu method. It was highlighted that yeast colony increased during the fermentation, while the pH level was decreasing. We found that the fermentation not only boosted some elements in primary metabolites, but also increased G. verrucosa bioactive groups. After 72 hours of fermentation, the G. verrucosa percent radical scavenging activity (%RSA) increased more than two times compared to the fresh G. verrucosa with a %RSA value of 16.09±6.57. Nevertheless, the highest total phenolic content of 5.62±0.00028 mg GAE/g extract was shown by G. verrucosa after 48 hours of fermentation.

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“…[1] Because of their biological importance, analytical methods to determine AAs have been an active research area for a long time. In the recent years, the commonly used techniques for AA analysis are based on spectroscopic, [2] electrochemical [3] or chromatographic [4] approaches.…”

Section: Introductionmentioning

confidence: 99%

Selective and direct detection of free amino acid using the optical birefringent patterns of confined nematic liquid crystals

Wei

Jang

2016

Liquid Crystals

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Detection of amino acid (AA) is an essential step to understand various biological processes. In this study, we used innovative optical birefringent patterns of nematic liquid crystals (LCs) for the detection of several AAs. We attempted to use capillary-confined nematic LCs as sensor for AA analysis because their three-dimensional micro-scale architecture of LCs allowed better performance than that of mainly reported LC thin film sensors owing to the effect on the formation and dynamics of point defect. The sensing system was built by disrupting the alignment of a nematic LC, 4-cyano-4′-pentylbiphenyl (5CB), using the dopant of dodecyl aldehyde. Detection principle is based on the chemical analytical method of aldehyde titration for AAs, wherein the reaction between AAs and aldehyde group generates Schiff bases that could alter the configuration of nematic LCs at the aqueous/LC interface. The patterns generated in the reaction are captured by polarising optical microscopy (POM) and are visible to the naked eye. The functionalised LCs detected glycine at concentrations as low as 1 pM. There was a surprising result that it can selectively detect D-AAs against their L-isomers, however, further efforts are required to explain the mechanism.ARTICLE HISTORY

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Modified ninhydrin reagents to detect amino acids on TLC plates

Cited by 13 publications

References 14 publications

Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Separation of Amino Acids, Dyes, and Pigments Using Novel Pressurized Circular TLC Assembly for Secure Medical Imaging Applications

Metabolites Alteration and Antioxidant Activity of Gracilaria verrucosa After Fermentation Using Aureobasidium melanogenum MTGK.31

Selective and direct detection of free amino acid using the optical birefringent patterns of confined nematic liquid crystals

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