Identification and incidence of porcine circovirus in routine field cases in Québec as determined by PCR

Larochelle, Renée; Morin, M; Antaya, M.; Magar, Ronald

doi:10.1136/vr.145.5.140

Cited by 57 publications

(55 citation statements)

References 13 publications

(13 reference statements)

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“…Combined infection with PRRSV of the European genotype and PCV2 was found in the lymphoid organs of 31% pigs out of the 39 examined. Natural occurrence of both viruses in pig herds has been reported previously (Larochelle et al 1999; but our study did not confirm PRRSV infection in any of the PMWS-affected pigs. High proportion of natural occurrence of PRRSV infection in the group of PMWS non-affected pigs could be the cause of low findings of actual PMWS cases, as similar clinical manifestations of respiratory diseases and wasting in the categories of young pigs in the course of PRRSV infection could have influenced an adequate selection of pigs for laboratory examinations.…”

Section: Discussioncontrasting

confidence: 50%

Exploratory Epidemiological Study on Porcine Circovirus Type 2 Infection and Postweaning Multisystemic Wasting Syndrome in the Czech Republic

Ficek¹,

Pšikal

Fictum

et al. 2010

Acta Vet. Brno

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The objective of our study was to diagnose the postweaning multisystemic wasting syndrome (PMWS) and to determine the prevalence of the disease in 33 swine herds in the Czech Republic using the results of laboratory examinations of 100 pigs expressing the signs of wasting at the end of 2007. Microscopic lesions associated with the presence of porcine circovirus 2 (PCV2) antigen were detected in the lymph nodes from 39 of 100 diseased pigs (39%). Based on individual assessment of severity of microscopic lymphoid lesions associated with high amounts of PCV2 antigen, PMWS was confirmed in 4 out of 39 pigs originating from 3 of 33 herds (9%). The epidemiological study indicates that PCV2 infections associated with PMWS disease are only sporadically present in the Czech Republic. Subsequently used real time PCR technique confirmed the relation between PMWS status at the individual pig level and PCV2 DNA concentration. PCV2 DNA load in lymph nodes of PMWS-affected pigs were about 3 logs higher than the levels detected in the PMWS-nonaffected group (P < 0.05). Other parallel viral infections (PRRSV, PPV) were detected by real time PCR techniques in 21 out of 39 PCV2 infected pigs (54%). The results of serological examination of blood samples collected during the necropsy of 100 pigs are suggestive of great prevalence of PCV2 infections in pig herds; nevertheless serum samples collected from individual pigs at a single point in time had a low diagnostic value.

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Section: Discussioncontrasting

confidence: 50%

Exploratory Epidemiological Study on Porcine Circovirus Type 2 Infection and Postweaning Multisystemic Wasting Syndrome in the Czech Republic

Ficek¹,

Pšikal

Fictum

et al. 2010

Acta Vet. Brno

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“…2,9 PCV2 antigens/nucleic acid have also been demonstrated in clinically normal animals from farms with no history of wasting disease. 11,15 Nevertheless a number of diagnostic procedures for PCV2-related disease syndromes have been reported that do not appear to take the possibility of subclinical PCV2 infections into account. 11,14,15 These studies have focused on standard block-based polymerase chain reaction (PCR) detection of PCV2 nucleic acid or infectious virus in tissue samples and suffer from the drawback of being nonquantitative and incapable of differentiating between clinical and subclinical infections.…”

mentioning

confidence: 99%

“…11,15 Nevertheless a number of diagnostic procedures for PCV2-related disease syndromes have been reported that do not appear to take the possibility of subclinical PCV2 infections into account. 11,14,15 These studies have focused on standard block-based polymerase chain reaction (PCR) detection of PCV2 nucleic acid or infectious virus in tissue samples and suffer from the drawback of being nonquantitative and incapable of differentiating between clinical and subclinical infections. 15 Although the use of block-based PCR was applicable in the early stages of defining the infectious agents present in tissue samples from animals with this newly recognized disease syndrome, 14 it is now known that subclinical infections, with minimal amounts of PCV2 present in tissues, are common in pigs and that PCV2-associated lesions in diseased animals contain an abundance of virus particles.…”

mentioning

confidence: 99%

Evaluation of a Porcine Circovirus Type 2–Specific Antigen-Capture Enzyme-Linked Immunosorbent Assay for the Diagnosis of Postweaning Multisystemic Wasting Syndrome in Pigs: Comparison with Virus Isolation, Immunohistochemistry, and the Polymerase Chain Reaction

McNeilly¹,

McNair²,

O'connor³

et al. 2002

J VET Diagn Invest

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Abstract. Quantitative virus isolation, immunohistochemistry, polymerase chain reaction (PCR) assay, and a porcine circovirus 2 (PCV2)-specific antigen-capture enzyme-linked immunosorbent assay (ELISA) were used for differentiation between clinical and subclinical PCV2 infections of swine. Tissue samples from pigs experimentally infected with PCV2 and field cases of postweaning multisystemic wasting syndrome and PCV2-associated reproductive disorders were used in this evaluation. In initial studies on 6 PCV2 pools using 3 previously published PCR protocols for PCV2 detection, quantitative virus isolation, and antigen-capture ELISA, substantial differences in sensitivity were identified among these procedures. Examination of tissue samples from diseased and clinically normal pigs indicated that immunohistochemistry, quantitative virus isolation, and antigen-capture ELISA could be used to differentiate between clinical and subclinical PCV2 infections, but the PCR assay could not. Because subclinical infections of pigs with PCV2 are common, the use of nonquantitative PCR as a diagnostic tool for PCV2-related diseases should be discouraged and the PCV2-specific antigencapture ELISA evaluated further.

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“…O vírus pode ser detectado por imunohistoquímica , hibridização "in situ" (Nawagaitgul et al 2000), imunofluorescência indireta , teste de reação em cadeia da polimerase -PCR (Larochelle et al 1999, Calsamiglia et al 2002 e por isolamento viral (Tischer et al 1982).…”

Section: Resul II Resul Ii Resul Ii Resul Ii Results T T T Tadunclassified

Circovirose suína

França

Ribeiro²,

Cunha³

et al. 2005

Pesq. Vet. Bras.

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Por meio de revisão da literatura pertinente foram coligidos e são apresentados os principais dados relativos aos aspectos epidemiológicos, clínicos, anátomo e histopatológicos observados na infecção por Circovírus Porcino tipo 2 (PCV-2) em suínos. São abordados a Síndrome Definhante Multissistêmica dos Suínos Desmamados (SDMDS), o Tremor Congênito Suíno (TCS), a Síndrome da Nefropatia e Dermatite Porcina (SNDP), bem como outras enfermidades associadas ou correlatas, a Síndrome Respiratória e Reprodutiva Porcina (SRRP), a Pneumonia Necrotizante Proliferativa (PNP) e as falhas reprodutivas. Uma vez que a SDMSD já foi registrada na Região Sul do Brasil e no Estado do Rio de Janeiro esse estudo objetiva chamar a atenção para o especial significado dessa virose para a suinocultura brasileira, em função dos prejuízos econômicos por ela determinados.

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Identification and incidence of porcine circovirus in routine field cases in Québec as determined by PCR

Cited by 57 publications

References 13 publications

Exploratory Epidemiological Study on Porcine Circovirus Type 2 Infection and Postweaning Multisystemic Wasting Syndrome in the Czech Republic

Exploratory Epidemiological Study on Porcine Circovirus Type 2 Infection and Postweaning Multisystemic Wasting Syndrome in the Czech Republic

Evaluation of a Porcine Circovirus Type 2–Specific Antigen-Capture Enzyme-Linked Immunosorbent Assay for the Diagnosis of Postweaning Multisystemic Wasting Syndrome in Pigs: Comparison with Virus Isolation, Immunohistochemistry, and the Polymerase Chain Reaction

Circovirose suína

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