Evaluation of a Porcine Circovirus Type 2–Specific Antigen-Capture Enzyme-Linked Immunosorbent Assay for the Diagnosis of Postweaning Multisystemic Wasting Syndrome in Pigs: Comparison with Virus Isolation, Immunohistochemistry, and the Polymerase Chain Reaction

McNeilly, F.; McNair, I.; O'connor, Mary L.; Brockbank, Simon; Gilpin, D.F.; Lasagna, C.; Boriosi, G.; Meehan, Brian; Ellis, John; Krakowka, S; Allan, Gordon

doi:10.1177/104063870201400203

Cited by 25 publications

(12 citation statements)

References 14 publications

(48 reference statements)

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“…Comparably, a previous study of McNeilly et al . [39] with PCV2 indicated that immunostaining of PCV2 is a more sensitive technique than virus isolation for the detection of PCV2 in porcine tissues.…”

Section: Discussionmentioning

confidence: 99%

Outcome of experimental porcine circovirus type 1 infections in mid-gestational porcine foetuses

et al. 2011

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BackgroundPorcine circovirus type 1 (PCV1) has been described as a non-cytopathic contaminant of the PK-15 cell line. Several experimental infections with PCV1 failed to reproduce disease in pigs. Therefore, PCV1 is generally accepted as non-pathogenic to pigs. To our knowledge, nothing is known about the outcome of PCV1 infections in porcine foetuses. This was examined in the present study.ResultsNine foetuses from three sows were inoculated at 55 days of gestation: three with 104.3 TCID50 of the PCV1 cell culture strain ATCC-CCL33, three with 104.3 TCID50 of the PCV1 field strain 3384 and three with cell culture medium (mock-inoculated). At 21 days post-inoculation, all 6 PCV1-inoculated and all 3 mock-inoculated foetuses had a normal external appearance. Microscopic lesions characterized by severe haemorrhages were observed in the lungs of two foetuses inoculated with CCL33. High PCV1 titres (up to 104.7 TCID50/g tissue) were found in the lungs of the CCL33-inoculated foetuses. All other organs of the CCL33-inoculated foetuses and all the organs of the 3384-inoculated foetuses were negative (< 101.7 TCID50/g tissue) by virus titration. PCV1-positive cells (up to 121 cells/10 mm2 in CCL33-inoculated foetuses and up to 13 cells/10 mm2 in 3384-inoculated foetuses) were found in the heart, lungs, spleen, liver, thymus and tonsils. PCR and DNA sequencing of Rep recovered CCL33 or 3384 sequences from CCL33- or 3384-inoculated foetuses, respectively.ConclusionsFrom this study, it can be concluded that cell culture PCV1 can replicate efficiently and produce pathology in the lungs of porcine foetuses inoculated at 55 days of foetal life.

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Section: Discussionmentioning

confidence: 99%

Outcome of experimental porcine circovirus type 1 infections in mid-gestational porcine foetuses

et al. 2011

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“…Porcine circovirus type 2 (PCV2) was first discovered in Canada in 1997. It often causes weaning piglets with multiple system failure syndrome (PMWS) and was tested using quantitative PCR and antigen capture ELISA [16]. Porcine Reproductive and Respiratory Syndrome virus (PRRSV) mainly causes reproductive disorders in pigs and first isolated in China in 1996.…”

Section: Introductionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Jiang¹,

Jiang

et al. 2020

Preprint

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KEYWORDS 2 swine disease, microfluidic chips, PCR, multiplex and on-site detection 3 Abstract Background: At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process takes a rather long time, has the risks of degradation of biological samples and generation of pathogenic microorganisms, and does not meet the rapid on-site detection demand. Therefore, development of a technology for rapid and high-throughput detection of pathogenic microorganisms at the customs port is of great significance. This study was to develop a microfluidic chip to be applied to rapid high-throughput detection for swine disease with higher accuracy and lower risk of spreading pathogenic microorganisms during transportation. Results: PCR technology has the advantages of high accurate and sensitivity in disease detection, clinical testing and food quarantine, so it plays an important role in customs inspection. However, the traditional PCR detection instrument has a large size, is timeconsuming and has strict requirements on the experimental environment, which greatly limit its application in on-site testing. In this paper, the positive nucleic acid of four swine diseases were detected by a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Eight clinical samples were detected together on the microfluidic chip in the system, and the detection results were obtained in about an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results show that the high sensitivity and specificity of the microfluidic PCR detection system in disease detection will play an important role in customs inspection and quarantine during customs clearance. Conclusion: The microfluidic PCR detection system established in this study could meet the requirements for rapid detection of samples at the customs port The new method can avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reducing the inspection cycle, and would enable parallel inspections on one chip which greatly raising the efficiency of inspection.Corporation for their support of chip design and fabrication and microchip based PCR detection instrument. Authors' contributionsJY designed and directed the study. JS and JY perform the experiment ,collect results and analyze data. YW and BZ draft the first vision of the manuscript. KMW, CG and DXZ collect clinical serum samples. BZ critically revised the manuscripts. All the participants carefully reviewed this vision manuscript. Ethics approval and consent to participate 11Not applicable Consent for publicationNot applicable Competing interest. Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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“…The virion has no envelope, and contains a covalently closed single-strand circular negativestrand DNA. It often causes weaning piglets with multiple system failure syndrome (PMWS) and was tested using quantitative PCR and antigen capture ELISA [16]. Porcine reproductive and respiratory syndrome (PRRSV) is a member of the Arteritis virus family.…”

Section: Introductionmentioning

confidence: 99%

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Jiang¹,

Jiang

et al. 2020

Preprint

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Background： At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process had the risks of degradation of biological samples and generation of pathogenic microorganisms and did not meet the rapid on-site detection demand because it took a rather long time. Therefore, it is urgently needed to develop a rapid and high-throughput detection assay of pathogenic microorganisms at the customs port. The aim of this study was to develop a microfluidic chip to rapidly detect swine pathogenic microorganisms with high-throughput and higher accuracy. Moreover, this chip will decrease the risk of spreading infection during transportation.Results: A series of experiments were performed to establish a microfluidic chip. The resulting data showed that the positive nucleic acid of four swine viruses were detected by using a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Furthermore, the detection results of eight clinical samples were obtained within an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results showed that the high sensitivity and specificity of this chip system in disease detection played an important role in customs inspection and quarantine during customs clearance.Conclusion: The microfluidic PCR detection system established in this study could meet the requirement for rapid detection of samples at the customs port. This chip could avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reduce the inspection cycle. Moreover, it would enable parallel inspections on one chip, which greatly raised the efficiency of inspection.

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Evaluation of a Porcine Circovirus Type 2–Specific Antigen-Capture Enzyme-Linked Immunosorbent Assay for the Diagnosis of Postweaning Multisystemic Wasting Syndrome in Pigs: Comparison with Virus Isolation, Immunohistochemistry, and the Polymerase Chain Reaction

Cited by 25 publications

References 14 publications

Outcome of experimental porcine circovirus type 1 infections in mid-gestational porcine foetuses

Outcome of experimental porcine circovirus type 1 infections in mid-gestational porcine foetuses

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

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