Exploratory Epidemiological Study on Porcine Circovirus Type 2 Infection and Postweaning Multisystemic Wasting Syndrome in the Czech Republic

Abstract

The objective of our study was to diagnose the postweaning multisystemic wasting syndrome (PMWS) and to determine the prevalence of the disease in 33 swine herds in the Czech Republic using the results of laboratory examinations of 100 pigs expressing the signs of wasting at the end of 2007. Microscopic lesions associated with the presence of porcine circovirus 2 (PCV2) antigen were detected in the lymph nodes from 39 of 100 diseased pigs (39%). Based on individual assessment of severity of microscopic lymphoid lesions associated with high amounts of PCV2 antigen, PMWS was confirmed in 4 out of 39 pigs originating from 3 of 33 herds (9%). The epidemiological study indicates that PCV2 infections associated with PMWS disease are only sporadically present in the Czech Republic. Subsequently used real time PCR technique confirmed the relation between PMWS status at the individual pig level and PCV2 DNA concentration. PCV2 DNA load in lymph nodes of PMWS-affected pigs were about 3 logs higher than the levels detected in the PMWS-nonaffected group (P < 0.05). Other parallel viral infections (PRRSV, PPV) were detected by real time PCR techniques in 21 out of 39 PCV2 infected pigs (54%). The results of serological examination of blood samples collected during the necropsy of 100 pigs are suggestive of great prevalence of PCV2 infections in pig herds; nevertheless serum samples collected from individual pigs at a single point in time had a low diagnostic value.

Immunoglobulins in Colostrum of Sows With Porcine Reproductive and Respiratory Syndrome - PRRS

“…However Shimizu, et al (1996) say that, PRRS virus caused neither alteration of T-cell subpopulation nor cell proliferation. Ficek, et al (2010) detected PRRS virus as parallel viral infection with the presence of porcine circovirus 2. In agreement with actual knowledge can PRRS virus mark up producing of cytokine IL-10, and that result in a inhibition of function of macrophages and T-lymphocytes [7].…”

Selenomethionine: A Pink Trojan Redox Horse with Implications in Aging and Various Age-Related Diseases

“…Moreover, SeMet has been reported to inhibit porcine circovirus type 2 (PCV2) in vitro in a concentration-dependent fashion between 2 and 16 µM. PCV2 is a notorious single-stranded DNA virus associated with several complications, including porcine dermatitis nephropathy syndrome (PDNS), postweaning multisystemic wasting syndrome (PMWS), porcine respiratory disease complex (PRDC), congenital tremor type A2 (CT) and pregnant sow reproductive obstruction [63][64][65]. The large differences in concentrations of SeMet required for antiviral activity in these studies against single-stranded RNA and DNA viruses may denote some selectivity of this RSeS towards the later type of viruses, albeit more studies are required to confirm this trend.…”