Identification and Histological Observation of Endophytic Fungi Isolated from Medicinal Plant, Physalis angulata L.

Abstract

Some endophytic fungi species live in medicinal plant tissue and does not make any damage, but live in symbiotic mutualism relationship with the host plant. This research was done to: 1) identify the endophytic fungi species isolated from P. angulata leaf, twig, and stem bark tissues, 2) determine the endophytic fungi colonization in the P. angulata plant tissue by histologic observation. The endophytic fungi was isolated from healthy P. angulata plant parts, then inoculated on Potato Dextrose Agar medium and incubated in 27°C for 7-14 days. Each endophytic fungi isolates were identified. The histologic observation was done by microscopic observation to determine the endophytic fungi position in the plant tissue. The conclusion are: 1) seven endophytic fungi species were found: Penicillium verrucosum, Colletotrichum alienum, Fusarium subglutinans, Aspergillus nidulans, Mycelia sterilia 1, Mycelia sterilia 2, and Rhizoctonia sp.; 2) the endophytic fungi micelium was found on the leaf epidermis cell wall, on the twig epidermis cell wall, and parenchyma cell wall, on the stem bark epidermis cell wall. The suggestion of the study: it is need to make the next research about secondary metabolites content produced by endophytic fungi species isolated from P. angulata and their antimicrobial activity.

Endophytic fungi: isolated from Cosmos caudatus Kunth and Cosmos sulphureus Cav.: a histologic observation, identification, and secondary metabolites chemical analysis

“…C. aenigma have been isolated from Persea americana [12]. C. alienum could be found as one of some endophytic fungi species in Michelia champaca L. [13]; in Physalis angulata L. leaf [14]; in Cananga odorata leaf and flower petal [15]; and Malus domestica plant [12]. C. aotearoa also found as an endophytic fungi species ini Cananga odorata [15].…”

Endophytic Fungi Isolated from Jasminum sambac L.: Identification, Histological Observation, and Content Analysis of Secondary Metabolites

“…Colletotrichum alienum and Colletotrichum kahawae was also found as endophytic fungi in Cananga odorata, a sort of plant that the flower also used for ritual ceremony as well as J. sambac [6]. Colletotrichum kahawae also found in Cordilyne fratiosa plant [14] and Switenia mahagoni [8] The research about endophytic fungi in Hedychium acuminatum also reported that Colletotrichum alienum and Rhizoctomia sp. Also found in this plant [14] and found in Physalis angulata [7].…”

“…Colletotrichum kahawae also found in Cordilyne fratiosa plant [14] and Switenia mahagoni [8] The research about endophytic fungi in Hedychium acuminatum also reported that Colletotrichum alienum and Rhizoctomia sp. Also found in this plant [14] and found in Physalis angulata [7].…”

“…Based on the previous research, the endophytic fungi position in the host plant tissue, i.e. : in the leaf, twig, and bark tissues of Tripterygium wilfordii plant [5]; in the flower petal, leaf, and twig tissues of Hedycium acuminatum plant [6]; in the leaf and twig of Physalis angulata plant [7]. The identification research result about endophytic fungi in Switenia mahagony plant twigs and leaves were found eight species: Mycelia sterilia, Hansfordia biophila, Rhizoctonia sp., Colletotrichum asianum, Colletotrichum siamense, Colletotrichum kahawae, Colletotrichum theobromicola, and Colletotrichum gloeosporioides [8].…”