Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii)

Li, Jinhua; Sima, Wei; Ouyang, Bo; Luo, Zhidan; Yang, Changxian; Ye, Zhibiao; Li, Hanxia

doi:10.1007/s11105-012-0509-4

Cited by 9 publications

(18 citation statements)

References 32 publications

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“…As shown in Figure S2, the Os2H16 promoter was expressed primarily in young root, anther and endosperm. The GFP fluorescence pattern in transgenic rice plants was similar to the previously reported expression pattern of the Os2H16 gene (Li et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…In addition, there was no obvious CAAT box next to the TATA-box on either strand. The Os2H16 promoter also contains two GT-1 elements, GAAAAA (Park et al, 2004) on the negative strand, three auxin-responsive elements, CTTTA and GTCTC (Mironova et al, 2014), two GA responsive elements, CCTTTT (Woodger et al, 2003) and four ABA responsive elements, ACACG, ACCCG and ACGTG (Li et al, 2013b). On the opposite strand of the Os2H16 promoter, we found one element that is complementary to the TGACG element in association with the as-1 element (Bacha et al, 2015;Krawczyk et al, 2002) and six CANNTG elements that are recognized by the helix-loop-helix (bHLH) TF superfamily (Wang et al, 2013c).…”

Section: Resultsmentioning

confidence: 99%

“…As previously reported (Li et al ., ), after 21 dpi with Xoo , lesion length was measured on T 0 transgenic lines and WT plants. Lesion length was measured on infected sheaths from transgenic and WT plants at 7 dpi with R. solani YWK196.…”

Section: Methodsmentioning

confidence: 99%

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OsASR2 regulates the expression of a defence‐related gene, Os2H16, by targeting the GT‐1 cis‐element

Wei

Chen

et al. 2017

Plant Biotechnology Journal

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SummaryThe GT‐1 cis‐element widely exists in many plant gene promoters. However, the molecular mechanism that underlies the response of the GT‐1 cis‐element to abiotic and biotic stresses remains elusive in rice. We previously isolated a rice short‐chain peptide‐encoding gene, Os2H16, and demonstrated that it plays important roles in both disease resistance and drought tolerance. Here, we conducted a promoter assay of Os2H16 and identified GT‐1 as an important cis‐element that mediates Os2H16 expression in response to pathogen attack and osmotic stress. Using the repeated GT‐1 as bait, we characterized an abscisic acid, stress and ripening 2 (ASR2) protein from yeast‐one hybridization screening. Sequence alignments showed that the carboxy‐terminal domain of OsASR2 containing residues 80–138 was the DNA‐binding domain. Furthermore, we identified that OsASR2 was specifically bound to GT‐1 and activated the expression of the target gene Os2H16, as well as GFP driven by the chimeric promoter of 2 × GT‐1‐35S mini construct. Additionally, the expression of OsASR2 was elevated by pathogens and osmotic stress challenges. Overexpression of OsASR2 enhanced the resistance against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani, and tolerance to drought in rice. These results suggest that the interaction between OsASR2 and GT‐1 plays an important role in the crosstalk of the response of rice to biotic and abiotic stresses.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

OsASR2 regulates the expression of a defence‐related gene, Os2H16, by targeting the GT‐1 cis‐element

Wei

Chen

et al. 2017

Plant Biotechnology Journal

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“…HyPRP1 also bound with ZPR1 protein, which is involved in the ABA signaling network and plays a potential role in plant cell development and abiotic stress response in tomato (Li et al, 2013 ). ZPR1 was initially identified in mammals as a cytoplasmic zinc finger protein, which is essential for cell viability and normal cellular proliferation (Gangwani et al, 1998 ).…”

Section: Discussionmentioning

confidence: 99%

HyPRP1 Gene Suppressed by Multiple Stresses Plays a Negative Role in Abiotic Stress Tolerance in Tomato

Ouyang

Wang

et al. 2016

Front. Plant Sci.

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Many hybrid proline-rich protein (HyPRP) genes respond to biotic and abiotic stresses in plants, but little is known about their roles other than as putative cell-wall structural proteins. A HyPRP1 gene encodes a protein with proline-rich domain, and an eight-cysteine motif was identified from our previous microarray experiments on drought-tolerant tomato. In this study, the expression of the HyPRP1 gene in tomato was suppressed under various abiotic stresses, such as drought, high salinity, cold, heat, and oxidative stress. Transgenic functional analysis showed no obvious changes in phenotypes, but enhanced tolerance to various abiotic stresses (e.g., oxidative stress, dehydration, and salinity) was observed in RNAi transgenic plants. Interestingly, several SO2 detoxification-related enzymes, including sulfite oxidase, ferredoxins (Fds), and methionine sulfoxide reductase A (Msr A), were revealed in HyPRP1-interacting proteins identified by Yeast Two-Hybrid screening. More sulfates and transcripts of Msr A and Fds were accumulated in HyPRP1 knockdown lines when wild-type plants were exposed to SO2 gas. Our findings illustrate that the tomato HyPRP1 is a negative regulator of salt and oxidative stresses and is probably involved in sulfite metabolism.

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“…TaC4ZFP contains two C4-type zinc fingers, known as the ZPR1 domain. Recently, a ZPR1 protein from wild tomato has been shown to be a transcriptional activator and binds to an ABA-responsive element ( Li et al , 2013 ). Regulation of this family of transcription factors by a Hsf is previously unknown.…”

Section: Discussionmentioning

confidence: 99%

TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets

Gong

Drenth

McIntyre

2014

Journal of Experimental Botany

150

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Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii)

Cited by 9 publications

References 32 publications

OsASR2 regulates the expression of a defence‐related gene, Os2H16, by targeting the GT‐1 cis‐element

OsASR2 regulates the expression of a defence‐related gene, Os2H16, by targeting the GT‐1 cis‐element

HyPRP1 Gene Suppressed by Multiple Stresses Plays a Negative Role in Abiotic Stress Tolerance in Tomato

TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets

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