TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets

Gong, Xue; Drenth, Janneke; McIntyre, C. Lynne

doi:10.1093/jxb/eru462

Cited by 150 publications

(78 citation statements)

References 85 publications

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“…Activities of SOD, APX and CAT in transgenic chrysanthemum overexpressing CmHsfA4, were higher than those of WT lines under salt stress . In addition, Hsf activates the expression of downstream genes (including Hsps) and shows a similar pattern of expression to protect plants from external stress (Snyman and Cronjé, 2008;Xue et al, 2014;Xue et al, 2015;Chen et al, 2018). In the present study, we also observed that transgenic Arabidopsis and NHE S. alfredii expressing SaHsfA4c showed higher CAT, POD, and APX activities than those of the WT after Cd stress, and were able to induce the expression of ROS-scavenging system related genes (POD, CAT and APX) and Hsps.…”

Section: Discussionsupporting

confidence: 77%

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Chen

et al. 2020

Front. Plant Sci.

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The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup from Sedum alfredii. Designated as SaHsfA4c, the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression of SaHsfA4c in yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcript InSaHsfA4c which contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function of SaHsfA4c under Cd stress was characterized in transgenic Arabidopsis and nonhyperaccumulation ecotype S. alfredii. SaHsfA4c was able to rescue the Cd sensitivity of the Arabidopsis athsfa4c mutant. SaHsfA4c reduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes and Hsps in transgenic lines. The present results suggest that SaHsfA4c increases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression of Hsps.

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Section: Discussionsupporting

confidence: 77%

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Chen

et al. 2020

Front. Plant Sci.

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“…Plant adaptation to high-temperature environments requires transcriptional reprogramming of a series of genes involved in heat stress protection (Xue et al, 2015). HSPs are a family of target genes of HSF, some of which are known to act as chaperones preventing misfolding and aggregation of other proteins during heat stress (von .…”

Section: Discussionmentioning

confidence: 99%

Molecular regulation and physiological functions of a novel FaHsfA2c cloned from tall fescue conferring plant tolerance to heat stress

Wang

Huang

et al. 2016

Plant Biotechnology Journal

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SummaryHeat stress transcription factors (HSFs) compose a large gene family, and different members play differential roles in regulating plant responses to abiotic stress. The objectives of this study were to identify and characterize an A2‐type HSF, FaHsfA2c, in a cool‐season perennial grass tall fescue (Festuca arundinacea Schreb.) for its association with heat tolerance and to determine the underlying physiological functions and regulatory mechanisms of FaHsfA2c imparting plant tolerance to heat stress. FaHsfA2c was localized in nucleus and exhibited a rapid transcriptional increase in leaves and roots during early phase of heat stress. Ectopic expression of FaHsfA2c improved basal and acquired thermotolerance in wild‐type Arabidopsis and also restored heat‐sensitive deficiency of hsfa2 mutant. Overexpression of FaHsfA2c in tall fescue enhanced plant tolerance to heat by triggering transcriptional regulation of heat‐protective gene expression, improving photosynthetic capacity and maintaining plant growth under heat stress. Our results indicated that FaHsfA2c acted as a positive regulator conferring thermotolerance improvement in Arabidopsis and tall fescue, and it could be potentially used as a candidate gene for genetic modification and molecular breeding to develop heat‐tolerant cool‐season grass species.

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“…A similar modulation of HSFs and HSPs was found in tomato in response to heat, drought and salinity (Fragkostefanakis et al ., ). In wheat, overexpression of Triticum aestivum HSFA6f was demonstrated to direct the expression of several HSPs , leading to thermotolerance (Xue et al ., ). Most strikingly, the expression of a maize HSF , ZmHSF06 ( Zea mays HSF06 ), is sufficient to confer heat and drought stress resistance to Arabidopsis (Li et al ., ).…”

Section: Introductionmentioning

confidence: 97%

The heat‐shock protein/chaperone network and multiple stress resistance

Jacob

Hirt

Bendahmane

2017

Plant Biotechnology Journal

529

342

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SummaryCrop yield has been greatly enhanced during the last century. However, most elite cultivars are adapted to temperate climates and are not well suited to more stressful conditions. In the context of climate change, stress resistance is a major concern. To overcome these difficulties, scientists may help breeders by providing genetic markers associated with stress resistance. However, multistress resistance cannot be obtained from the simple addition of single stress resistance traits. In the field, stresses are unpredictable and several may occur at once. Consequently, the use of single stress resistance traits is often inadequate. Although it has been historically linked with the heat stress response, the heat‐shock protein (HSP)/chaperone network is a major component of multiple stress responses. Among the HSP/chaperone ‘client proteins’, many are primary metabolism enzymes and signal transduction components with essential roles for the proper functioning of a cell. HSPs/chaperones are controlled by the action of diverse heat‐shock factors, which are recruited under stress conditions. In this review, we give an overview of the regulation of the HSP/chaperone network with a focus on Arabidopsis thaliana. We illustrate the role of HSPs/chaperones in regulating diverse signalling pathways and discuss several basic principles that should be considered for engineering multiple stress resistance in crops through the HSP/chaperone network.

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TaHsfA6f is a transcriptional activator that regulates a suite of heat stress protection genes in wheat (Triticum aestivum L.) including previously unknown Hsf targets

Abstract: SummaryA wheat HsfA6 member acts as a transcriptional activator for up-regulation of a suite of heat stress protection genes including previously unknown Hsf targets such as Golgi anti-apoptotic protein.

Cited by 150 publications

References 85 publications

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Molecular regulation and physiological functions of a novel FaHsfA2c cloned from tall fescue conferring plant tolerance to heat stress

The heat‐shock protein/chaperone network and multiple stress resistance

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