OsASR2 regulates the expression of a defence‐related gene, Os2H16, by targeting the GT‐1 cis‐element

Abstract

SummaryThe GT‐1 cis‐element widely exists in many plant gene promoters. However, the molecular mechanism that underlies the response of the GT‐1 cis‐element to abiotic and biotic stresses remains elusive in rice. We previously isolated a rice short‐chain peptide‐encoding gene, Os2H16, and demonstrated that it plays important roles in both disease resistance and drought tolerance. Here, we conducted a promoter assay of Os2H16 and identified GT‐1 as an important cis‐element that mediates Os2H16 expression in response to pathogen attack and osmotic stress. Using the repeated GT‐1 as bait, we characterized an abscisic acid, stress and ripening 2 (ASR2) protein from yeast‐one hybridization screening. Sequence alignments showed that the carboxy‐terminal domain of OsASR2 containing residues 80–138 was the DNA‐binding domain. Furthermore, we identified that OsASR2 was specifically bound to GT‐1 and activated the expression of the target gene Os2H16, as well as GFP driven by the chimeric promoter of 2 × GT‐1‐35S mini construct. Additionally, the expression of OsASR2 was elevated by pathogens and osmotic stress challenges. Overexpression of OsASR2 enhanced the resistance against Xanthomonas oryzae pv. oryzae and Rhizoctonia solani, and tolerance to drought in rice. These results suggest that the interaction between OsASR2 and GT‐1 plays an important role in the crosstalk of the response of rice to biotic and abiotic stresses.

Copper ions suppress abscisic acid biosynthesis to enhance defence against Phytophthora infestans in potato

“…Water loss assay was performed with 10 potato plants for each treatment as described previously (Li et al , ). About 30 leaves of potato plants treated with CuSO 4 or MgSO 4 were harvested.…”

“…The cross‐linking reaction was stopped by adding glycine to a final concentration of 0.125 M and applying a vacuum for an additional 5 min. Subsequently, the chromatin was isolated and sonicated as described previously (Li et al , ). Anti‐FLAG monoclonal antibody (Abways) and Co‐immunoprecipitation Kit (Thermo) were used for immunoprecipitation.…”

“…The EMSA was performed as described previously (Li et al , ) with Chemiluminescent EMSA Kit (Beyotime). To express StEIN3 protein in E. coli BL21, the DNA fragment was amplified by PCR and inserted into the pMal‐c2X‐ Bam HI‐ Sal I‐cut vector.…”

Genome-wide characterization of the abscisic acid-, stress- and ripening-induced (ASR) gene family in wheat (Triticum aestivum L.)

“…Increasing evidences showed that ASR proteins play important roles in plant growth and fruit ripening [1,[25][26][27], as well as in regulation of floral development and flowering time [15,28,29]. The ASR proteins have been well documented for their responses to multiple abiotic stresses, including drought, salt, heat, cold, and exposure to cadmium (Cd) and aluminum (Al) [9,24,[30][31][32][33][34][35][36][37][38].…”

Transcriptome analysis of rice leaves in response to Rhizoctonia solani infection

“…Several previously reported genes that contribute resistance to ShB, such as OsACS2 (11), 14-3-3GF14f (35), OsPAL4 (36), OsPR4b (37), OsMYB4 (38), and OsASR2 (39), are present in our DEGs dataset. These results confirmed the effectiveness of our RNA-Seq experiment.…”