Identification and expression of a novel class of glutathione-S-transferase from amphioxus Branchiostoma belcheri with implications to the origin of vertebrate liver

Fan, Chunxin; Zhang, Shicui; Liu, Zhenhui; Li, Lei; Luan, Jing; Saren, Gaowa

doi:10.1016/j.biocel.2006.09.013

Cited by 64 publications

(28 citation statements)

References 32 publications

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“…The culture was diluted 1:1000 with LB broth and subjected to further incubation at 37°C for 2 h. The expression of BbIGF was induced by the addition of isopropyl β-D-thiogalactoside (IPTG) to the culture at a final concentration of 0.1 mM and the recominant protein was purified as previuously described (Fan et al 2007). The purity of the eluted samples was analysed by 12% SDS-polyacrylamide gel electrophoresis (PAGE); proteins were stained with Coomassie brilliant blue R-250.…”

Section: Expression and Purification Of Recombinant Proteinmentioning

confidence: 99%

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Expression, mitogenic activity and regulation by growth hormone of growth hormone/insulin-like growth factor in Branchiostoma belcheri

et al. 2009

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The growth hormone (GH)/insulin-like growth factor (IGF) axis is unique to all the vertebrate species but its evolutionary origin is ill-defined. We therefore cloned a cDNA encoding Branchiostoma belcheri IGF (BbIGF). BbIGF was expressed in a tissue-specific manner, with the most abundant expression in the hepatic caecum, the putative liver precursor. The recombinant BbIGF expressed in vitro showed mitogenic activity capable of stimulating cell proliferation in the flounder gill, a characteristic of vertebrate IGF. Quantitative real-time polymerase chain reaction demonstrated that the recombinant rat GH was able to induce a significant up-regulation of BbIGF expression in the hepatic caecum. Moreover, Western blotting revealed the presence of a molecule similar to rat GH receptor in the hepatic caecum. These results suggest that BbIGF expression is inducible by exogenous mammalian GH, suggesting the presence of a GH/IGF axis in B. belcheri. The relationship between BbIFG expression and the origin of the vertebrate liver is discussed.

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Section: Expression and Purification Of Recombinant Proteinmentioning

confidence: 99%

“…An aliquot of 5 μg RNAs was electrophoresed, blotted onto Nylon membrane (Roche) and hybridized as described by Fan et al (2007).…”

Section: Northern Blottingmentioning

confidence: 99%

Expression, mitogenic activity and regulation by growth hormone of growth hormone/insulin-like growth factor in Branchiostoma belcheri

et al. 2009

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“…Some classes of GSTs were found in several fish species (Leaver et al, 1997;Melgar Riol et al, 2001;Doi et al, 2004;Lee et al, 2005;Konishi et al, 2005b;Fan et al, 2007;Trute et al, 2007). A distinct GST isoform, which has no homologues in mammals, was found in fish and named as rho class in red sea bream (Pagrus major) (Konishi et al, 2005a).…”

Section: Introductionmentioning

confidence: 99%

The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)

Liang

Xie

et al. 2008

Aquatic Toxicology

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“…Using phylogenetic relationships inferred from Bayesian analyses, Lee et al [58] found that theta class GSTs formed two distinct, wellsupported clades, one ("theta A") containing only fish representatives and the other ("theta B") including theta-class GSTs from mammals, fish, birds, and invertebrates. Recent evidence has confirmed the deeply rooted nature of the "theta A' clade by the identification of a rho-GSTonce thought to be a fish-specific class [58]-in a primitive cephalochordate [59]. The Cyphoma GST theta-like peptides matched both rho-and theta-class fish sequences in the "theta A" clade, providing the first evidence for invertebrate members of this group.…”

Section: Discussionmentioning

confidence: 82%

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Whalen¹,

Morin²,

Lin³

et al. 2008

Archives of Biochemistry and Biophysics

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Short title: Characterization of glutathione S-transferases from Cyphoma gibbosumFootnote: The nucleotide and translated amino acid sequences for C. gibbosum GSTs have been deposited in GenBank with the following accession nos. EU008563 (CgGSTM1) and EU008562 (CgGSTM2).Abbreviations footnote: CDNB, 1-chloro-2,4-dinitrobenzene; GST, glutathione S-transferase; nrDB, non-redundant database; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase polymerase chain reaction 2 ABSTRACT Glutathione S-transferases (GST) were characterized from the digestive gland of Cyphoma gibbosum (Mollusca; Gastropoda), to investigate the possible role of these detoxification enzymes in conferring resistance to allelochemicals present in its gorgonian coral diet. We identified the collection of expressed cytosolic Cyphoma GST classes using a proteomic approach involving affinity chromatography, HPLC and nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two major GST subunits were identified as putative mu-class GSTs; while one minor GST subunit was identified as a putative theta-class GST, apparently the first theta-class GST identified from a mollusc. Two Cyphoma GST cDNAs (CgGSTM1 and CgGSTM2) were isolated by RT-PCR using primers derived from peptide sequences. Phylogenetic analyses established both cDNAs as mu-class GSTs and revealed a mollusc-specific subclass of the GST-mu clade. These results provide new insights into metazoan GST diversity and the biochemical mechanisms used by marine organisms to cope with their chemically defended prey.

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Identification and expression of a novel class of glutathione-S-transferase from amphioxus Branchiostoma belcheri with implications to the origin of vertebrate liver

Cited by 64 publications

References 32 publications

Expression, mitogenic activity and regulation by growth hormone of growth hormone/insulin-like growth factor in Branchiostoma belcheri

Expression, mitogenic activity and regulation by growth hormone of growth hormone/insulin-like growth factor in Branchiostoma belcheri

The effect of cyanobacterial crude extract on the transcription of GST mu, GST kappa and GST rho in different organs of goldfish (Carassius auratus)

Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

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