Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Whalen, Kristen E.; Morin, Dexter; Lin, Ching‐Yu; Tjeerdema, Ronald S.; Goldstone, Jared V.; Hahn, Mark E.

doi:10.1016/j.abb.2008.07.007

Cited by 24 publications

(13 citation statements)

References 71 publications

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“…The biochemical adaptations that involve a gene response against xenobiotics, as the GST activity, could partly explain the consumers' reluctance to food chemical threats and provide information about enzymatic mechanisms underlying foraging decisions [45], [54], [55]. GST induction with an allelochemical diet can serve as an additional adjustment mechanism of protection against toxicity [54], [55]; this phenomenon may be present in our study system (oyster-toxic dinoflagellate).…”

Section: Discussionmentioning

confidence: 93%

Genomics Study of the Exposure Effect of Gymnodinium catenatum, a Paralyzing Toxin Producer, on Crassostrea gigas' Defense System and Detoxification Genes

García-Lagunas¹,

Romero-Geraldo²,

Hernández-Saavedra³

2013

PLoS ONE

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Background Crassostrea gigas accumulates paralytic shellfish toxins (PST) associated with red tide species as Gymnodinium catenatum. Previous studies demonstrated bivalves show variable feeding responses to toxic algae at physiological level; recently, only one study has reported biochemical changes in the transcript level of the genes involved in C. gigas stress response.Principal FindingsWe found that 24 h feeding on toxic dinoflagellate cells (acute exposure) induced a significant decrease in clearance rate and expression level changes of the genes involved in antioxidant defense (copper/zinc superoxide dismutase, Cu/Zn-SOD), cell detoxification (glutathione S-transferase, GST and cytochrome P450, CPY450), intermediate immune response activation (lipopolysaccharide and beta glucan binding protein, LGBP), and stress responses (glutamine synthetase, GS) in Pacific oysters compared to the effects with the non-toxic microalga Isochrysis galbana. A sub-chronic exposure feeding on toxic dinoflagellate cells for seven and fourteen days (30×103 cells mL−1) showed higher gene expression levels. A significant increase was observed in Cu/Zn-SOD, GST, and LGBP at day 7 and a major increase in GS and CPY450 at day 14. We also observed that oysters fed only with G. catenatum (3×103 cells mL−1) produced a significant increase on the transcription level than in a mixed diet (3×103 cells mL−1 of G. catenatum+0.75×106 cells mL−1 I. galbana) in all the analyzed genes.ConclusionsOur results provide gene expression data of PST producer dinoflagellate G. catenatum toxic effects on C. gigas, a commercially important bivalve. Over expressed genes indicate the activation of a potent protective mechanism, whose response depends on both cell concentration and exposure time against these toxic microalgae. Given the importance of dinoflagellate blooms in coastal environments, these results provide a more comprehensive overview of how oysters respond to stress generated by toxic dinoflagellate exposure.

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Section: Discussionmentioning

confidence: 93%

Genomics Study of the Exposure Effect of Gymnodinium catenatum, a Paralyzing Toxin Producer, on Crassostrea gigas' Defense System and Detoxification Genes

García-Lagunas¹,

Romero-Geraldo²,

Hernández-Saavedra³

2013

PLoS ONE

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“…This same gene GST showed higher transcript levels 2.6 and 3.9-fold in DG of oysters exposed 29 h and 48 h to non-toxic A. tamarense , respectively (Figure 1). Glutathione S-transferase belongs to a large super-family of multi-functional enzymes primary involved in cellular detoxification by catalyzing the nucleophilic attack by glutathione (GSH) on electrophilic compounds with a wide range of endogenous and xenobiotic agents, including environmental toxins and oxidative stress products [22,23,24,25,26,27]. GST transcript abundance was previously demonstrated to be over expressed in oysters exposed to toxic and non toxic cells of the dinoflagellate Gymnodinium catenatum [17].…”

Section: Resultsmentioning

confidence: 99%

A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella

et al. 2014

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To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in caspase-3/7 activity and normal percentage of apoptotic cells in this tissue. Taken together, these results suggest a feedback mechanism, which may occur in the digestive gland where BI.1 could play a key role in preventing the induction of apoptosis by PSTs. Moreover, the expression of CYP1A, Bax and BI.1 were found to be significantly correlated to the occurrence of natural toxic events, suggesting that the expression of these genes together could be used as biomarker to assess the biological responses of oysters to stress caused by PSTs.

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“…Similarly, the theta class was responsive to organic compounds in the flounder Platichthys flesus, and has been identified as the most responsive cytosolic GST in fish challenged with organic compounds (Williams et al, 2008). This family was well represented in this transcriptome data although very little is known of this class in molluscs (Whalen et al, 2008). With regards to microsomal GSTs, six contigs were likely to be assigned to MAPEG family, with subgroups 1 (MGST1) and 3 (MGST3) most represented (Table 5).…”

Section: Transcripts Encoding Putative Glutathione S-transferases (Gsmentioning

confidence: 87%

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

et al. 2015

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Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana.Aquatic Toxicology http://dx.doi.org/10. 1016/j.aquatox.2015.06.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights-Thetranscriptome of the mangrove oyster Crassostrea brasilianawas sequenced.-The sequencing effort dramatically expanded the existing cDNA sequences available for the species.-Global analysis for transcription in the oyster treated with phenanthrene, diesel and domestic sewage was performed.-The pollutants altered mRNAs for genes in biotransformation, antioxidant and stress response pathways. Abstract 2The Brazilian oyster Crassostrea brasiliana was challenged to three common environmental contaminants: phenanthrene, diesel fuel water-accommodated fraction (WAF) and domestic sewage. Total RNA was extracted from the gill and digestive gland, and cDNA libraries were sequenced using the 454 FLX pla orm. The assembled transcriptome resulted in ˜20,000contigs, which were annotated to produce the first de novo transcriptome for C. brasiliana.Sequences were screened to identify genes potentially involved in the biotransformation of xenobiotics and associated antioxidant defence mechanisms. These gene families included those of the cytochrome P450 (CYP450), 70kDa heat shock, antioxidants, such as glutathione Stransferase, superoxide dismutase, catalase and also multi-drug resistance proteins. Analysis showed that the massive expansion of the CYP450 and HSP70 family due to gene duplication identified in the Crassostrea gigas genome also occurred in C. brasiliana, suggesting these processes form the base of the Crassostrea lineage. Preliminary expression analyses revealed several candidates biomarker genes that were up-regulated during each of the three treatments, suggesting the potential for environmental monitoring.

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Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

Cited by 24 publications

References 71 publications

Genomics Study of the Exposure Effect of Gymnodinium catenatum, a Paralyzing Toxin Producer, on Crassostrea gigas' Defense System and Detoxification Genes

Genomics Study of the Exposure Effect of Gymnodinium catenatum, a Paralyzing Toxin Producer, on Crassostrea gigas' Defense System and Detoxification Genes

A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

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