Identification and differential distribution of collagen types in the central and peripheral nervous systems

Shellswell, G. B.; Restall, D. J.; Duance, Victor Colin; Bailey, Allen J.

doi:10.1016/0014-5793(79)80520-7

Cited by 93 publications

(29 citation statements)

References 12 publications

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“…The cartilagenous elements are just beginning to form, and the extracellular matrix is still poorly developed with only small amounts of type I collagen present (27,28). A total of 40 embryos were injected with -1 pul of fluorescent collagen.…”

Section: Resultsmentioning

confidence: 99%

Morphogenetic rearrangement of injected collagen in developing chicken limb buds.

Stopak¹,

Wessells²,

Harris³

1985

Proc. Natl. Acad. Sci. U.S.A.

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A fundamental question concerning the development of the extracellular matrix is what factors control the arrangement of collagen fibrils within a tissue and at the same time allow for the great diversity of geometric forms exhibited by collagen. In this report, we test the possibility that physical forces within the embryo serve to organize collagen fibers into regular patterns. In particular, we test the prediction that patterns of stress having this morphogenetic function are generated by cell traction, the contractile force exerted by cells to propel themselves. To study the effects of these mechanical forces on the extracellular matrix, type I collagen was fluorescently labeled and injected into developing chicken wing buds. When the injected limbs were allowed to develop and then examined histologically, the exogenous collagen was found incorporated within normal connective tissues of the wing. The labeled collagen became arranged according to its site of injection, forming parts of tendons, perichondria, cartilages, perineuria, and blood vessels. Since the injected collagen formed a gel within minutes of its injection, the subsequent incorporation of this preformed collagen within organized structures cannot be explained in terms of molecular self-assembly or other mechanisms occurring during collagen deposition. These results demonstrate that, within developing tissues, patterns of forces exist that are capable of physically rearranging collagen and determining its long-range order.During embryonic development, collagen somehow becomes organized into a wide variety of different spatial patterns whose mechanical properties lend structural support and give form to vertebrate tissues. Although much is known about the self-assembly of collagen molecules into fibrils (1, 2), comparatively little is known about how collagen fibrils become associated together into the diverse supramolecular arrangements found in the body. We In collagen matrices, the compressive and tensile strains generated by cell traction bring about a rearrangement of collagen fibers. This realignment, in turn, affects cell behavior so that positive feedback cycles arise that spontaneously generate regular arrangements of cells and matrix (5-7). By controlling such simple factors as the initial cell distribution or the resistance of the collagen gel to distortion, a number of different connective tissue structures can be created in culture, including the pattern of dermal condensations, which make up the feather tracts of birds and pelage hair of mammals (6), the tendon and ligament attachments of muscles to long bones (5), and the arrangement of collagen into capsules or sheets, which cover many organs (5). Insofar as they have been tested in situ, the existence of these morphogenetic effects has been confirmed. During wound healing (18), in the development of the cardiac cushion of the heart (19), and during amphibian gastrulation (20), rearrangement of extracellular fibers has been observed to follow the migration of cells into...

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Section: Resultsmentioning

confidence: 99%

Morphogenetic rearrangement of injected collagen in developing chicken limb buds.

Stopak¹,

Wessells²,

Harris³

1985

Proc. Natl. Acad. Sci. U.S.A.

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“…For optical microscopy, cryostat sections are submitted to an indirect immunofluorescent staining and studied under a fluorescence microscope (SHELLSWELL et al, 1979).…”

Section: Immunohistologymentioning

confidence: 99%

Biology of collagen-proteoglycan interaction.

Junqueira

Montes

1983

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Although the major constituent of basal laminae is collagen IV (together with laminin), the presence of fibrillar collagens of type I, III, and V has also been demonstrated (Shellswell et al, 1979;Azzi et al, 1989). These types are grouped as fibrillar collagens, since they tend to form supramolecular linear fibrils, in contrast, for example, to the thin, sheet-like structures formed by collagen IV (Vuorio and de Crombrugghe, 1990).…”

Section: Discussionmentioning

confidence: 99%

Astrocytes in culture express fibrillar collagen

Heck

Garwood

Schütte

et al. 2003

Glia

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The use of monoclonal antibodies has led to much progress in the characterization of extracellular matrix components of the CNS. F1C3 is a monoclonal antibody raised against the astrocytic cell line, Neu7. Analysis by immunoprecipitation and Western blots of the F1C3 antigen in Neu7 cell lysates and conditioned medium reveals a recognition of several protein bands around 140 -230 kD. Internal peptide sequence data from these bands indicate that they are highly homologous to fibrillar collagens, and the F1C3 antigen is specifically digested by the collagenase I protease. Other glial cell lines show F1C3 antigen expression including A7, C6, and U373. Cultures of neonatal primary astrocytes also express F1C3 antigen, and Western blot analysis of rat brain extracts from different ages and parts of the brain confirm an in vivo expression of F1C3 protein. The significance of the expression of fibrillar collagenlike proteins by astrocytes is discussed together with its possible implication during developmental processes and in the context of CNS lesions and regeneration. GLIA 41: 382-392, 2003.

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Identification and differential distribution of collagen types in the central and peripheral nervous systems

Cited by 93 publications

References 12 publications

Morphogenetic rearrangement of injected collagen in developing chicken limb buds.

Morphogenetic rearrangement of injected collagen in developing chicken limb buds.

Biology of collagen-proteoglycan interaction.

Astrocytes in culture express fibrillar collagen

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