Biology of collagen-proteoglycan interaction.

Previous studies have suggested that sympathetic sprouting in the periphery may contribute to the development and persistence of sympathetically maintained pain in animal models of neuropathic pain. In the present study, we examined changes in the cutaneous innervation in rats with a chronic constriction injury to the sciatic nerve. At several periods postinjury, hind paw skin was harvested and processed by using a monoclonal antibody against dopamine‐β‐hydroxylase to detect sympathetic fibers and a polyclonal antibody against calcitonin gene‐related peptide to identify peptidergic sensory fibers. We observed migration and branching of sympathetic fibers into the upper dermis of the hind paw skin, where they were normally absent. This migration was first detected at 2 weeks, peaked at 4–6 weeks, and lasted for at least 20 weeks postlesion. At 8 weeks postlesion, there was a dramatic increase in the density of peptidergic fibers in the upper dermis. Quantification revealed that densities of peptidergic fibers 8 weeks postlesion were significantly above levels in sham animals. The ectopic sympathetic fibers did not innervate blood vessels but formed a novel association and wrapped around sprouted peptidergic nociceptive fibers. Our data show a long‐term sympathetic and sensory innervation change in the rat hind paw skin after the chronic constriction injury. This novel fiber arrangement after nerve lesion may play an important role in the development and persistence of sympathetically maintained neuropathic pain after partial nerve lesions. J. Comp. Neurol. 495:679–690, 2006. © 2006 Wiley‐Liss, Inc.

show abstract

“…This layer is the most basal layer of the epidermis and consists of a single layer of (Junqueira and Carniero, 1992;Gartner and Hiatt, 2001). …”

Section: Stratum Basale (Stratum Germinativum)mentioning

confidence: 99%

Sympathetic sprouting and changes in nociceptive sensory innervation in the glabrous skin of the rat hind paw following partial peripheral nerve injury

Yen

Bennett

Ribeiro‐da‐Silva

2006

J of Comparative Neurology

103

102

View full text Add to dashboard Cite

show abstract

“…These molecules have been proposed as functional non-covalent linkages within the ECM [23][24][25][26]. Aggrecan, the major proteoglycan in cartilage, while distinct from the CN, may also form interactions with the CN through hyaluronic acid, or its keratan sulfate-and chondroitin sulfate-rich regions [27][28][29][30]. Together, extrafibrillar matrix components may mediate the adherence and sliding between collagen fibrils to modulate cartilage tissue shape [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Articular cartilage tensile integrity: Modulation by matrix depletion is maturation-dependent

Asanbaeva

Tam

Schumacher

et al. 2008

Archives of Biochemistry and Biophysics

View full text Add to dashboard Cite

Articular cartilage function depends on the molecular composition and structure of its extracellular matrix (ECM). The collagen network (CN) provides cartilage with tensile integrity, but must also remodel during growth. Such remodeling may depend on matrix molecules interacting with the CN to modulate the tensile behavior of cartilage. The objective of this study was to determine the effects of increasingly selective matrix depletion on tensile properties of immature and mature articular cartilage, and thereby establish a framework for identifying molecules involved in CN remodeling. Depletion of immature cartilage with guanidine, chondroitinase ABC, chondroitinase AC, and Streptomyces hyaluronidase markedly increased tensile integrity, while the integrity of mature cartilage remained unaltered after depletion with guanidine. The enhanced tensile integrity after matrix depletion suggests that certain ECM components of immature matrix serve to inhibit CN interactions and may act as modulators of physiological alterations of cartilage geometry and tensile properties during growth/maturation.

show abstract

“…Although the regeneration of teleost fins has been the subject of several papers (NABRIT,1929;PRENANT, 1938;BLANC,1949;GOSS and STAGG,1957;KEMP and PARK, 1970;WEIS and WETS, 1980), these reports do not provide comprehensive ultrastructural data and detailed histochemical information concerning the regenerating fin skeleton. Since in the last two decades remarkable advances in connective tissue research methodology have been made (for reviews see, JUNQUEIRA and MONTES, 1983;MONTES et al, 1984;JUNQUEIRA, 1988, 1991), it appeared expedient to undertake a systematic study of the regeneration of fin rays in light of current concepts about extracellular matrix components.…”

mentioning

confidence: 99%

Regeneration of Fin Rays in Teleosts: A Histochemical, Radioautographic, and Ultrastructural Study.

Becerra

Junqueira

Bechara

et al. 1996

Archives of Histology and Cytology

Self Cite

View full text Add to dashboard Cite

Biology of collagen-proteoglycan interaction.

Cited by 86 publications

References 51 publications

Sympathetic sprouting and changes in nociceptive sensory innervation in the glabrous skin of the rat hind paw following partial peripheral nerve injury

Sympathetic sprouting and changes in nociceptive sensory innervation in the glabrous skin of the rat hind paw following partial peripheral nerve injury

Articular cartilage tensile integrity: Modulation by matrix depletion is maturation-dependent

Regeneration of Fin Rays in Teleosts: A Histochemical, Radioautographic, and Ultrastructural Study.

Contact Info

Product

Resources

About