Identification and comparison of three carp fishes based on mitochondrial 16S rRNA gene

Jahan, Hawa; Akter, Maria; Begum, Rowshan Ara; Shahjahan, Reza Md

doi:10.3329/dujbs.v26i2.46396

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…In molecular identification, mitochondrial COI, 16SrRNA, 12SrRNA and cyt-b are used frequently. Jahan et al (2017) have studied 16SrRNA gene sequence of three Labeo species viz., L. rohita, L. bata and L. gonius and found 23 polymorphic sites (5.8%) in 396 nucleotide long sequence. The polymorphic site variations (23.5%) are more frequent than that reported by Jahan et al (2017) because of larger size sequences, higher number of species (eight) and use of different region of mitochondrial genome (part of cyt-b) in the present study.…”

Section: Resultsmentioning

confidence: 99%

Partial sequence analysis of mitochondrial cytochrome B gene of Labeo calbasu of Bangladesh

Begum

Alam

Jahan

et al. 2019

J. Biodivers. Conserv. Bioresour. Manag.

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Labeo calbasu (Family Cyprinidae) was studied at DNA level to know genetic diversity within and between species. The mitochondrial cytochrome b (cyt-b) gene of L. calbasu was sequenced and compared to the corresponding sequences of other Labeo species. DNA was isolated from the tissue sample of L. calbasu using phenol: chloroform extraction method. Forward and reverse primers were designed to amplify the target region of cytochrome b gene. A standard PCR protocol was used for the amplification of the desired region. Then, the forward and reverse sequences obtained were aligned and edited to finalize a length of 510 nucleotides which was submitted to NCBI genbank database. Nucleotide BLAST of this sequence at NCBI resulted 100% sequence similarity with L. calbasu sequence of the same region of cyt-b gene. Multiple sequence alignment of the sequence with seven more Labeo species sequences revealed 120 polymorphic sites, which have been mark of diversity among the species and might be used in molecular identification of the Labeo species. A constructed phylogenetic tree has shown relationship among the Labeo species. This research demonstrated the usefulness of mitochondrial DNA-based approach in species identification. Further, the data will provide appropriate background for studying genetic diversity within-species of the Labeo species in general and of L. calbasu in particular. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(1): 25-30

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Section: Resultsmentioning

confidence: 99%

Partial sequence analysis of mitochondrial cytochrome B gene of Labeo calbasu of Bangladesh

Begum

Alam

Jahan

et al. 2019

J. Biodivers. Conserv. Bioresour. Manag.

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show abstract

“…This is due to the use of 16S mitochondrial DNA as a molecular marker has several advantages such as maternal mode of inheritance, limited recombination, and high conserve which can be used to study species identification and interspecies relationship [8]. The use of 16S mitochondrial marker had been successfully applied to identify species identification [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Determination of species boundaries of Selais fish from Arut River, Central Kalimantan based on 16S mitochondrial gene using Bayesian approach

et al. 2020

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Selais fish is difficult to discriminate with other Silurid fish species based on morphological characters. As a result, the valid species of selais fish is uncertain. Therefore, a molecular phylogenetic study was needed to clarify species boundaries and to addresses genetic relationships of the selais fish. In this study, 16S mitochondrial gene of ten selais samples collected from Arut River (Central Kalimantan) were sequenced, from which a Bayesian trees was generated. Result revealed monophyletic of selais fish which is revealed as a single species. The Bayesian inference showed that the selais fish clade is distinguished with two other genus, Kryptopterus and Ompok, by its sequence differences. This finding can address species boundaries of selais fish using Bayesian approach, but the name of the selais species has not been clarified.

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“…Therefore, in this study we use partial 16S mitochondrial gene as a molecular marker to detect polymorphism in the spotted barb fish. The 16S mitochondrial gene has been widely used to identify and detect genetic variation of freshwater fish such as carp fishes (Jahan et al, 2017) and kissing gourami (Arisuryanti et al, 2019 a ), Serranidae fish (Saad, 2019), and red sea parrotfish (Saad et al, 2019). The finding data can be also utilised to arrange 16S mt-DNA library sequences of the spotted barb fish from Indonesia.…”

Section: Introductionmentioning

confidence: 99%

Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara

Arisuryanti

Alfianti

Firdaus

et al. 2020

JFS

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The spotted barb fish (Barbodes binotatus), a tropical cyprinid fish, is one of the abundant fishes in Lake Lebo Taliwang commonly consumed due to high nutrition and utilized as an aquarium trade due to their exotic colour. However, no genetic information related to the intra-population genetic variation of the fish species in Lake Lebo Taliwang. Genetic information of the spotted barb fish can play an important role for the fish conservation. Therefore, the objective of this study was to detect 16S mitochondrial gene polymorphism of the spotted barb fish which can be used to detect their genetic variation. The method used in this study was a PCR method with primers 16Sar and 16Sbr. The data was then analysed using MESQUITE, MEGA, DnaSP and PopART to identify 16S mitochondrial gene polymorphism. This study obtained 610 base pairs after alignment of 16S sequences of all samples. Five haplotypes were detected with 9 variable sites and 2 parsimony informative sites. Haplotype diversity and nucleotide diversity of the spotted barb fish were 1.00 and 0.0066 respectively. In addition, genetic distance among the samples was 0.66. This finding exhibited polymorphism on 16S gene sequence among the fish samples and this data indicated genetic variation of the spotted barb from Lake Lebo Taliwang.

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Identification and comparison of three carp fishes based on mitochondrial 16S rRNA gene

Cited by 7 publications

References 16 publications

Partial sequence analysis of mitochondrial cytochrome B gene of Labeo calbasu of Bangladesh

Partial sequence analysis of mitochondrial cytochrome B gene of Labeo calbasu of Bangladesh

Determination of species boundaries of Selais fish from Arut River, Central Kalimantan based on 16S mitochondrial gene using Bayesian approach

Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara

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