Detection of 16S Mitochondrial Gene Polymorphism on Barb Fish (Barbodes binotatus Valenciennes, 1842) from Lake Lebo Taliwang, West Nusa Tenggara

Abstract

The spotted barb fish (Barbodes binotatus), a tropical cyprinid fish, is one of the abundant fishes in Lake Lebo Taliwang commonly consumed due to high nutrition and utilized as an aquarium trade due to their exotic colour. However, no genetic information related to the intra-population genetic variation of the fish species in Lake Lebo Taliwang. Genetic information of the spotted barb fish can play an important role for the fish conservation. Therefore, the objective of this study was to detect 16S mitochondrial gene polymorphism of the spotted barb fish which can be used to detect their genetic variation. The method used in this study was a PCR method with primers 16Sar and 16Sbr. The data was then analysed using MESQUITE, MEGA, DnaSP and PopART to identify 16S mitochondrial gene polymorphism. This study obtained 610 base pairs after alignment of 16S sequences of all samples. Five haplotypes were detected with 9 variable sites and 2 parsimony informative sites. Haplotype diversity and nucleotide diversity of the spotted barb fish were 1.00 and 0.0066 respectively. In addition, genetic distance among the samples was 0.66. This finding exhibited polymorphism on 16S gene sequence among the fish samples and this data indicated genetic variation of the spotted barb from Lake Lebo Taliwang.

Ikan Nyalian (Barbodes binotatus Valenciennes, 1842) di Danau Tamblingan, Buleleng, Bali Dilihat dari Aspek Makanannya

“…Ikan nyalian (Barbodes binotatus) sebagai salah satu anggota famili Cyprinidae merupakan ikan air tawar dengan warna bervariasi dari abu-abu keperakan sampai abu-abu kehijauan, dimana bagian punggung lebih gelap dan bagian perut lebih pucat (Arisuryanti et al, 2020). Dalam literatur ilmiah, ikan nyalian memiliki nama sinonim, diantaranya Puntius binotatus, Capoeta binotata, Systomus binotatus, Barbus maculatus (Kottelat et al, 2013).…”

HUBUNGAN PANJANG-PANJANG, POLA PERTUMBUHAN DAN KONDISI IKAN NYALIAN, Barbodes binotatus (VALENCIENNES, 1842) DI EMPAT DANAU DI PULAU BALI

“…B. binotatus tersebar luas di perairan tawar negara-negara asia tenggara, termasuk Indonesia (Kottelat, 2013). Spesies ini tersebar hampir di seluruh perairan tawar Indonesia seperti, Sumatera (Situmorang et al, 2013;Karlina et al, 2016), Jawa Nuryanto et al, 2016;Trijoko et al, 2016;Haryono dan Wahyudewantoro 2020), Kalimantan (Pratama et al, 2018), Sulawesi (Parenti et al, 2014), Nusa Tenggara Barat (Arisuryanti et al, 2020) dan Bali (Dahruddin et al, 2016). B. binotatus dapat mendiami berbagai habitat perairan tawar seperti sungai, danau, air terjun, dan aliran gunung (Nuryanto et al, 2016).…”

“…Penelitian yang mengungkapkan informasi biologis B. binotatus sudah cukup banyak dipublikasikan, beberapa diantaranya terkait morfologi (Vitri et al, 2012), morfometrik tradisional dan geometrik (Fahmi-Ahmad et al, 2020), hubungan panjang-bobot dan faktor kondisi (Jusmaldi & Hariani 2018), laju pertumbuhan pada kepadatan stok berbeda (Prasetyo et al, 2019), komparasi jenis makanan (Situmorang et al, 2013), uji kandungan pakan terhadap laju pertumbuhan (Sutarjo et al, 2020), pengaruh ethinylestradiol terhadap sperma B. binotatus , dinamika populasi (Batubara et al, 2019), dan genetik (Dahruddin et al, 2016;Arisuryanti et al, 2020). Informasi tekait B. binotatus di perairan danau di Bali sudah dipublikasikan beberapa penelitian sebelumnya.…”