Selais fish is difficult to discriminate with other Silurid fish species based on morphological characters. As a result, the valid species of selais fish is uncertain. Therefore, a molecular phylogenetic study was needed to clarify species boundaries and to addresses genetic relationships of the selais fish. In this study, 16S mitochondrial gene of ten selais samples collected from Arut River (Central Kalimantan) were sequenced, from which a Bayesian trees was generated. Result revealed monophyletic of selais fish which is revealed as a single species. The Bayesian inference showed that the selais fish clade is distinguished with two other genus, Kryptopterus and Ompok, by its sequence differences. This finding can address species boundaries of selais fish using Bayesian approach, but the name of the selais species has not been clarified.
Rimbo Panti Nature Reserve is a conservation area located in West Sumatra which consisted of two main habitat types, dry hilly and swampy forest habitat types. These forest types potentially board many species of mammals. This research was conducted from December 2015 to March 2016 in The Nature Reserve to determine the diversity of mammals occupied each type of habitats. Twenty two camera traps were installed in pairs, six pairs in the dry hilly habitat (574-871 m asl) and five pairs in the swampy habitat (216-261 m asl). From a total 109 photos, 17 species were identified, consisted of 11 species from hilly and 10 species from swampy forest habitat. There were four species found in both habitat types. Shannon-Wiener diversity index showed that mammalian diversity of Rimbo Panti Nature Reserve is moderate (H’=1.95) while mammalian diversity in swampy forest (H’= 2.06) was higher than hilly habitat forest (H’=1.54).
Selais fish belongs to famili Siluridae consisting of 12 genera with 104 properly validated species. The human need for these fish has sharply increased due to the benefits provided especially for consumption. However, morphologically the selais fish are slightly difficult to differentiate among other silurid fish for non-specialist experts. Thus, a DNA barcoding approach using the mitochondrial COI gene as a molecular marker in this study was applied to clarify a taxonomic status and classification species of selais fish from Arut River (Central Kalimantan, Indonesia) and was also to assembly COI database library of the fish from Indonesia. The method used in this study was a PCR method with a pair of universal primers FishF2 and FishR2. Based on COI-based DNA barcoding, the whole samples showed there were no sequence differences (only 1 haplotype) within the population and this confirmed that these fish only consisted of one identical species. Furthermore, phylogenetic analysis (NJ / ML / BI) revealed that selais fish in this study had a close genetic relationship with Ompok hypophthalmus compared to other Ompok groups. This relationship was supported by the genetic distance value not exceeding 3.6% and this evaluated the undetermined naming of the selais fish from Arut River which was previously still unclassifiable.
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