Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples

Chen, An-Jing; Pi, Jin-Kui; Hu, Jun-Gen; Huang, Yizhou; Gao, Hong‐Wei; Li, Shengfu; Li‐Ling, Jesse; Xie, Huiqi

doi:10.1007/s11427-018-9543-1

Cited by 32 publications

(45 citation statements)

References 43 publications

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“…Our study demonstrated that the isolated cell from canine urine was positive for CD29, CD44, CD90, and CD73, while lacking expression of CD45, CD11b, and CD34, which corresponds to the MSC surface markers specified by the International Society for Cellular Therapy (ISCT) [ 34 ]. The expressions of these surface markers were consistent with USCs from humans [ 39 , 42 ].…”

Section: Discussionsupporting

confidence: 61%

“…Our study showed that the isolated cUSCs adhered to plastic and displayed a spindle-shaped morphology, similar to those reported from humans[ 15 , 39 ]. Previous studies reported that USCs derived from human urine (hUSCs) proliferated to 70-80% confluence no more than 3 days [ 16 , 40 ], while the other two studies indicated that USCs derived from humans proliferated to 70-80% confluence no more than 5 days [ 41 , 42 ]. Similarly, our study showed that after 5 days, the cUSCs proliferated to 70-80% confluence.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells

Zhang

Chen

et al. 2020

Stem Cells International

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Current cell-based therapies on musculoskeletal tissue regeneration were mostly determined in rodent models. However, a direct translation of those promising cell-based therapies to humans exists a significant hurdle. For solving this problem, canine has been developed as a new large animal model to bridge the gap from rodents to humans. In this study, we reported the isolation and characterization of urine-derived stem cells (USCs) from mature healthy beagle dogs. The isolated cells showed fibroblast-like morphology and had good clonogenicity and proliferation. Meanwhile, these cells positively expressed multiple markers of MSCs (CD29, CD44, CD90, and CD73), but negatively expressed for hematopoietic antigens (CD11b, CD34, and CD45). Additionally, after induction culturing, the isolated cells can be differentiated into osteogenic, adipogenic, chondrogenic, and tenogenic lineages. The successful isolation and verification of USCs from canine were useful for studying cell-based therapies and developing new treatments for musculoskeletal injuries using the preclinical canine model.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells

Zhang

Chen

et al. 2020

Stem Cells International

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“…Human USCs expressed a variety of markers, and their proposed phenotypes vary according to studies, urine sample and likewise between single colonies appearing in a single urine sample[ 20 , 27 , 28 ].…”

Section: Isolation and Characterization Of Urine Stem Cellsmentioning

confidence: 99%

“…Regarding their origin, USCs express adhesion markers such as CD29 and/or CD166 but not CD31. They also express some renal specific transporters such as AQP1 and/or AQP2[ 20 ]. Importantly, some studies show that at least a subpopulation of USC doubly express CD24/CD133.…”

Section: Isolation and Characterization Of Urine Stem Cellsmentioning

confidence: 99%

Urine-derived stem/progenitor cells: A focus on their characterization and potential

Burdeyron

Giraud

Hauet

et al. 2020

WJSC

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Cell therapy, i.e ., the use of cells to repair an affected tissue or organ, is at the forefront of regenerative and personalized medicine. Among the multiple cell types that have been used for this purpose [including adult stem cells such as mesenchymal stem cells or pluripotent stem cells], urine-derived stem cells (USCs) have aroused interest in the past years. USCs display classical features of mesenchymal stem cells such as differentiation capacity and immunomodulation. Importantly, they have the main advantage of being isolable from one sample of voided urine with a cheap and unpainful procedure, which is broadly applicable, whereas most adult stem cell types require invasive procedure. Moreover, USCs can be differentiated into renal cell types. This is of high interest for renal cell therapy-based regenerative approaches. This review will firstly describe the isolation and characterization of USCs. We will specifically present USC phenotype, which is not an object of consensus in the literature, as well as detail their differentiation capacity. In the second part of this review, we will present and discuss the main applications of USCs. These include use as a substrate to generate human induced pluripotent stem cells, but we will deeply focus on the use of USCs for cell therapy approaches with a detailed analysis depending on the targeted organ or system. Importantly, we will also focus on the applications that rely on the use of USC-derived products such as microvesicles including exosomes, which is a strategy being increasingly employed. In the last section, we will discuss the remaining barriers and challenges in the field of USC-based regenerative medicine.

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“…MI is a major cause of mortality and morbidity worldwide (Paiva et al, 2015). Effective treatment strategies for MI include limiting adverse ventricular remodeling, attenuating myocardial scar expansion, and enhancing cardiac functions and myocardial regeneration (Chen et al, 2020;Perea-Gil et al, 2015;Dixit and Katare, 2015).…”

Section: Introductionmentioning

confidence: 99%

Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells

Wang

et al. 2020

Sci. China Life Sci.

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Stem cell therapy is an attractive approach for recovery from myocardial infarction (MI) but faces the challenges of rapid diffusion and poor survival after transplantation. Here we developed an injectable collagen scaffold to promote the long-term retention of transplanted cells in chronic MI. Forty-five minipigs underwent left anterior descending artery (LAD) ligation and were equally divided into three groups 2 months later (collagen scaffold loading with human umbilical mesenchymal stem cell (hUMSC) group, hUMSC group, and placebo group (only phosphate-buffered saline (PBS) injection)). Immunofluorescence staining indicated that the retention of transplanted cells was promoted by the collagen scaffold. Echocardiography and cardiac magnetic resonance imaging (CMR) showed much higher left ventricular ejection fraction (LVEF) and lower infarct size percentage in the collagen/hUMSC group than in the hUMSC and placebo groups at 12 months after treatment. There were also higher densities of vWf-, α-sma-, and cTnT-positive cells in the infarct border zone in the collagen/cell group, as revealed by immunohistochemical analysis, suggesting better angiogenesis and more cardiomyocyte survival after MI. Thus, the injectable collagen scaffold was safe and effective on a large animal myocardial model, which is beneficial for constructing a favorable microenvironment for applying stem cells in clinical MI. injectable collagen scaffold, mesenchymal stem cell, MI

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Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples

Cited by 32 publications

References 43 publications

Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells

Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells

Urine-derived stem/progenitor cells: A focus on their characterization and potential

Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells

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