Identification and characterization of three PeSHRs and one PeSCR involved in adventitious root development of Populus

Liu, Xuan; Xu, Meng; Chen, Caihui; Yang, Chunxia; Xu, Liang; Huang, Minren

doi:10.1007/s11240-014-0437-0

Cited by 20 publications

(15 citation statements)

References 31 publications

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“…The gene structure analysis indicated that these three genes were all intron-free. Similar results were obtained in Populus [ 31 ]. The Prosite reports have suggested that the three proteins had a typical GRAS domain, thereby confirming that the three proteins belong to the plant-specific GRAS family of TFs [ 35 ].…”

Section: Discussionsupporting

confidence: 89%

“…Expression patterns of these three genes showed that expression of the genes ThSHR2 and ThSCR was highest at the period of S2, then began to decline, while that of gene ThSHR1 exhibited steady increases in expression throughout the process of adventitious root formation and development. The expression pattern of ThSHR1 is similar to the previous reports, while ThSHR2 and ThSCR are not exactly consistent with previous studies [ 31 ]. The possible reasons are differences in design and differences in species.…”

Section: Discussionsupporting

confidence: 77%

“…On the whole, the results of this study are consistent with previous reports [ 32 ]. Consistent with their role as transcriptional regulators, the ThSHR1-GFP, ThSHR2-GFP and ThSCR-GFP fusion proteins were located in the nucleus, similar to the situation in Populus cells [ 31 ]. Combined together, these features provide strong evidence that the three genes isolated here encode Taxodium GRAS family transcription factors.…”

Section: Discussionmentioning

confidence: 68%

“…The protein sequences of ThSHR1 , ThSHR2 and ThSCR were compared with Arabidopsis protein sequences in TAIR (AtSHR, At4g37650.1; AtSCR, At3g54220.1) and Populus protein sequences based on previous studies [ 28 , 29 , 30 , 31 ]. The result indicated that the two SHR proteins and one SCR protein belonged to the plant-specific GRAS family of transcription factors and exhibited considerable sequence similarity to one other throughout their carboxyl termini, including leucine heptad repeat I (LHRI), the VHIID motif, leucine heptad repeat II (LHRII), the PFYRE motif and the SAW motif ( Figure 2 and Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

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Cloning and Characterization of ThSHRs and ThSCR Transcription Factors in Taxodium Hybrid ‘Zhongshanshan 406’

Wang

Yin

Hua

et al. 2017

Genes

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Among the GRAS family of transcription factors, SHORT ROOT (SHR) and SCARECROW (SCR) are key regulators of the formation of root tissues. In this study, we isolated and characterized two genes encoding SHR proteins and one gene encoding an SCR protein: ThSHR1 (Accession Number MF045148), ThSHR2 (Accession Number MF045149) and ThSCR (Accession Number MF045152) in the adventitious roots of Taxodium hybrid ‘Zhongshanshan’. Gene structure analysis indicated that ThSHR1, ThSHR2 and ThSCR are all intron free. Multiple protein sequence alignments showed that each of the corresponding proteins, ThSHR1, ThSHR2 and ThSCR, contained five well-conserved domains: leucine heptad repeat I (LHRI), the VHIID motif, leucine heptad repeat II (LHR II), the PFYRE motif, and the SAW motif. The phylogenetic analysis indicated that ThSCR was positioned in the SCR clade with the SCR proteins from eight other species, while ThSHR1 and ThSHR2 were positioned in the SHR clade with the SHR proteins from six other species. Temporal expression patterns of these genes were profiled during the process of adventitious root development on stem cuttings. Whereas expression of both ThSHR2 and ThSCR increased up to primary root formation before declining, that of ThSHR1 increased steadily throughout adventitious root formation. Subcellular localization studies in transgenic poplar protoplasts revealed that ThSHR1, ThSHR2 and ThSCR were localized in the nucleus. Collectively, these results suggest that the three genes encode Taxodium GRAS family transcription factors, and the findings contribute to improving our understanding of the expression and function of SHR and SCR during adventitious root production, which may then be manipulated to achieve high rates of asexual propagation of valuable tree species.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Cloning and Characterization of ThSHRs and ThSCR Transcription Factors in Taxodium Hybrid ‘Zhongshanshan 406’

Wang

Yin

Hua

et al. 2017

Genes

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“…Broad genetic variation exists for adventitious rooting in Populus (Zalesny et al ), which provides the potential for realizing substantial selection gains. In recent years, some genes and signaling pathways associated with adventitious rooting have been identified in Populus (Ramírez‐Carvajal et al , Rigal et al , Xu et al , Xuan et al , Yang et al ).…”

Section: Introductionmentioning

confidence: 99%

Two WUSCHEL‐related HOMEOBOX genes, PeWOX11a and PeWOX11b, are involved in adventitious root formation of poplar

Xie

Huang

2015

Physiologia Plantarum

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The plant-specific WUSCHEL-related HOMEOBOX (WOX) transcription factors play important roles in key developmental processes, but knowledge regarding functional characterization of WOX genes in poplar remains limited. To reveal genes and signaling pathways associated with adventitious rooting in poplar, here we isolated and characterized two WOX genes through the rapid amplification of cDNA ends (RACE), sequence aligning, expression profiling, protoplast transfection and poplar transformation. Detailed information about the sequence similarity, structural features, evolutionary relationships, expression patterns and subcellular localization of the two genes were revealed. Overexpression of either PeWOX11a or PeWOX11b not only increased the number of adventitious roots on the cuttings but also induced ectopic roots in the aerial parts of transgenic poplars. Meanwhile, their overexpression in transgenic poplars affected axillary bud and leaf development. These results suggest that PeWOX11a and PeWOX11b were involved in multiple developmental processes of poplar, especially in adventitious root formation. Our results provide new insights into the molecular mechanisms underlying adventitious root formation of poplar.

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Isolation and characterization of a poplar d-type cyclin gene associated with the SHORT-ROOT/SCARECROW network

Liu

et al. 2015

Trees

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Identification and characterization of three PeSHRs and one PeSCR involved in adventitious root development of Populus

Cited by 20 publications

References 31 publications

Cloning and Characterization of ThSHRs and ThSCR Transcription Factors in Taxodium Hybrid ‘Zhongshanshan 406’

Cloning and Characterization of ThSHRs and ThSCR Transcription Factors in Taxodium Hybrid ‘Zhongshanshan 406’

Two WUSCHEL‐related HOMEOBOX genes, PeWOX11a and PeWOX11b, are involved in adventitious root formation of poplar

Isolation and characterization of a poplar d-type cyclin gene associated with the SHORT-ROOT/SCARECROW network

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